Bacillus subtilis as a bioindicator for estimating pentachlorophenol toxicity and concentration

Ayude, María Alejandra; Okada, Elena; Gonzalez, Jorge Froilán; Haure, Patricia M.; Murialdo, Silvia Elena

doi:10.1007/s10295-009-0550-y

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…The reported isolated strain was also capable of metabolizing chlorophenols (Wolski et al, 2006;Durruty et al, 2011aDurruty et al, , 2011b without the production of toxic byproducts (Ayude et al, 2009). In preliminary assays, we have also observed the growth of this strain in liquid mediums with cumene, hexanes, unleaded gasoline, and premium grade diesel fuel as the only carbon and energy source.…”

Section: Discussionmentioning

confidence: 59%

Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

Nisenbaum

Sendra

Gilbert

et al. 2013

Journal of Environmental Sciences

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We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 10(5) colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.

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Section: Discussionmentioning

confidence: 59%

Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

Nisenbaum

Sendra

Gilbert

et al. 2013

Journal of Environmental Sciences

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“…In a previous work, Murialdo et al [6] demonstrated that these strains were able to dechlorinate PCP completely. Subsequent work also demonstrated the absence of toxicity of the supernatant after degradation, towards a sensitive bacteria [18]. In this research, we studied the individual degradation of PCP, 2,4,6 TCP, and 2,3,5,6 TeCP by mixed culture in a batch reactor to evaluate the kinetic parameters for each substrate.…”

Section: Introductionmentioning

confidence: 99%

Multisubstrate monod kinetic model for simultaneous degradation of chlorophenol mixtures

Durruty

Okada

Gonzalez

et al. 2011

Biotechnol Bioproc E

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Chlorophenols (CPs) are persistent and highly toxic compounds rated as priority pollutants by the Environmental Protection Agency (EPA). Frequently, these compounds are present as mixtures of CPs in industrial wastewaters. Therefore the study of biodegradation on mixed pollutants is an important aspect of biodegradation and wastewater treatment. In this work, we studied the multisubstrate degradation of CPs by a mixed culture of Pseudomonas aeruginosa and a novel Acromobacter sp. capable of using pentachlorophenol (PCP), 2,4,6 trichlorophenol (2,4,6 TCP) and 2,3,5,6 tetrachlorophenol (2,3,5,6 TeCP) as the sole sources of carbon and energy. The main objective of this work was to evaluate the effect of substrate mixtures on the degradation kinetics of PCP. Batch experiments were conducted with each CP separately and in mixtures of PCP + 2,4,6 TCP, PCP + 2,3,5,6 TeCP, and PCP + 2,4,6 TCP + 2,3,5,6 TeCP. Based upon our results we have concluded that the simultaneous degradation of CPs is a key factor contributing to the improvement of PCP degradation. The kinetic parameters for PCP and 2,4,6 TCP were obtained by fitting the data to a Monod kinetics model. Using such parameters, the model was able to predict simultaneous multisubstrate degradation of PCP with others CPs.

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“…Although GC and HPLC provide reliable results at trace levels, these methods also have some disadvantages. require high-priced and sophisticated equipment (Ayude et al, 2009). Sample extraction and cleanup is required to achieve correct separation and quantitation.…”

Section: Existing Methods For Detecting Chlorinated Phenolsmentioning

confidence: 99%

Synthesis, sorption and sensing capability of carbon nanotube based materials for gas-phase chlorinated phenols

Kanchanatip

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The adsorption equilibrium isotherms as well as gas sensing of three chlorinated phenolic compounds (CPCs) including phenol, 2-chlorophenol, and 2,4-dichlorophenol by acid-functionalized carbon nanotubes (CNT) were studied. CNTs were synthesized via floating catalyst chemical vapor deposition method at 800oC and then functionalized with a mixture of HNO3 and H2SO4. The functionalized CNTs were vacuum filtered to form CNT buckypaper. The adsorption isotherm followed the Langmuir adsorption model better than the Freundlich and Redlich-Peterson models. The maximum adsorption capacities were found to be 61.35, 93.46 and 104.17 mg/g for phenol, 2-chlorophenol and 2,4-dichlorophenol, respectively. The desorption activation energy determined via thermogravimetric analysis indicates that adsorption of all three CPCs onto the CNTs belonged to chemisorption type. For the gas sensing test, the CNT buckypaper showed fast response to the tested CPCs with good stability and repeatability. The sensitivity values obtained were 13.778 × 10-4, 8.835 × 10-4 and 7.122 × 10-4 for 2,4-dichlorophenol, 2-chlorophenol and phenol, respectively. It is noteworthy that the adsorption capacity and sensitivity of the CNT based materials to the target gases increased with the increase in the number of chlorine atoms in the phenolic compounds.

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Bacillus subtilis as a bioindicator for estimating pentachlorophenol toxicity and concentration

Cited by 10 publications

References 9 publications

Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

Multisubstrate monod kinetic model for simultaneous degradation of chlorophenol mixtures

Synthesis, sorption and sensing capability of carbon nanotube based materials for gas-phase chlorinated phenols

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