Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein

Vetráková, A.; Chovanová, Romana Kalianková; Rechtorikova, Radoslava; Krajcikova, Daniela; Barák, Imrich

doi:10.1016/j.csbj.2023.02.007

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…Probiotics were proven to exert positive immunomodulation functions, such as enhancing secretory IgA (sIgA) and homeostasis of the immune system [ 47 ]. Amongst the studied probiotics utilized as host/vector systems include Lactobacillus casei , Lactobacillus lactis , the non-pathogenic strain of E. coli ; E. coli Nissle 1917, and B. subtilis [ 27 , 48 , 49 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

TonB-Dependent Receptor Protein Displayed on Spores of Bacillus subtilis Stimulates Protective Immune Responses against Acinetobacter baumannii

et al. 2023

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The emergence of antibiotic-resistant Acinetobacter baumannii strains with limited treatment options has become a significant global health concern. Efforts to develop vaccines against the bacteria have centred on several potential protein targets, including the TonB-dependent receptors (TBDRs). In the present study, TBDRs from A. baumannii were displayed on the surface of Bacillus subtilis spores. The immunogenicity of the recombinant spores was evaluated in orally vaccinated mice. None of the immunized mice demonstrated signs of illness and were observed to be healthy throughout the study. Sera and the intestinal secretions from the recombinant spores-treated mice demonstrated mucosal and humoral antibody responses to the vaccine antigen. In addition, bactericidal activities of the sera against A. baumannii clinical isolates were demonstrated. These observations suggest that the B. subtilis spore-displayed TBDRs should be further explored as much-needed potential oral vaccine candidates against A. baumannii.

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Section: Discussionmentioning

confidence: 99%

TonB-Dependent Receptor Protein Displayed on Spores of Bacillus subtilis Stimulates Protective Immune Responses against Acinetobacter baumannii

et al. 2023

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“…Hence, an oral dose may be a suitable alternative. The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein was fused to CotY or CotZ and was proposed to be used as an oral vaccine for the SARS-CoV-2 virus [ 16 ]. In another investigation, the spike protein of the ancestorial SARS-CoV-2 coronavirus was fused to CotC.…”

Section: Applicationsmentioning

confidence: 99%

Applications of Bacillus subtilis Protein Display for Medicine, Catalysis, Environmental Remediation, and Protein Engineering

Mahmoodi,

Farinas

2024

Microorganisms

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Bacillus subtilis spores offer several advantages that make them attractive for protein display. For example, protein folding issues associated with unfolded polypeptide chains crossing membranes are circumvented. In addition, they can withstand physical and chemical extremes such as heat, desiccation, radiation, ultraviolet light, and oxidizing agents. As a result, the sequence of the displayed protein can be easily obtained even under harsh screening conditions. Next, immobilized proteins have many economic and technological advantages. They can be easily separated from the reaction and the protein stability is increased in harsh environments. In traditional immobilization methods, proteins are expressed and purified and then they are attached to a matrix. In contrast, immobilization occurs naturally during the sporulation process. They can be easily separated from the reaction and the protein stability is increased in harsh environments. Spores are also amenable to high-throughput screening for protein engineering and optimization. Furthermore, they can be used in a wide array of biotechnological and industrial applications such as vaccines, bioabsorbants to remove toxic chemicals, whole-cell catalysts, bioremediation, and biosensors. Lastly, spores are easily produced in large quantities, have a good safety record, and can be used as additives in foods and drugs.

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“…Bacterial spores have been proposed for the oral delivery of the COVID-19 vaccine. In particular, Bacillus subtilis recombinant spores expressing the SARS-CoV-2 spike protein receptor-binding domain have been developed and shown to increase specific neutralizing antibodies when orally administrated to mice or healthy human volunteers [ 50 , 51 ]. The utilization of B. subtilis spores to display heterologous antigens has been extensively reported since its first successful application in 2001 by Isticato et al, and will be the focus of this review [ 52 ].…”

Section: Bacterial Spores As a Mucosal Vaccine Delivery Systemmentioning

confidence: 99%

“…Most antigens exposed on the spore surface have been anchored to the crust-associated component CotG [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ,…”

Section: Bacterial Spores As a Mucosal Vaccine Delivery Systemmentioning

confidence: 99%

Bacterial Spore-Based Delivery System: 20 Years of a Versatile Approach for Innovative Vaccines

Isticato

2023

Biomolecules

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Mucosal vaccines offer several advantages over injectable conventional vaccines, such as the induction of adaptive immunity, with secretory IgA production at the entry site of most pathogens, and needle-less vaccinations. Despite their potential, only a few mucosal vaccines are currently used. Developing new effective mucosal vaccines strongly relies on identifying innovative antigens, efficient adjuvants, and delivery systems. Several approaches based on phages, bacteria, or nanoparticles have been proposed to deliver antigens to mucosal surfaces. Bacterial spores have also been considered antigen vehicles, and various antigens have been successfully exposed on their surface. Due to their peculiar structure, spores conjugate the advantages of live microorganisms with synthetic nanoparticles. When mucosally administered, spores expressing antigens have been shown to induce antigen-specific, protective immune responses. This review accounts for recent progress in the formulation of spore-based mucosal vaccines, describing a spore’s structure, specifically the spore surface, and the diverse approaches developed to improve its efficiency as a vehicle for heterologous antigen presentation.

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Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein

Cited by 9 publications

References 52 publications

TonB-Dependent Receptor Protein Displayed on Spores of Bacillus subtilis Stimulates Protective Immune Responses against Acinetobacter baumannii

TonB-Dependent Receptor Protein Displayed on Spores of Bacillus subtilis Stimulates Protective Immune Responses against Acinetobacter baumannii

Applications of Bacillus subtilis Protein Display for Medicine, Catalysis, Environmental Remediation, and Protein Engineering

Bacterial Spore-Based Delivery System: 20 Years of a Versatile Approach for Innovative Vaccines

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