Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner)

Chakrabarti, S. K.; Mandaokar, Ajin; Shukla, Alok; Pattanayak, Debasis; Naik, Prakash S.; Sharma, Rameshwar; Kumar, Pankaj

doi:10.1007/bf02357955

Cited by 20 publications

(5 citation statements)

References 21 publications

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“…However, several other crops have successfully been transformed to express different cry genes for protection against this pest. These include tobacco (Selvapandiyan et al, 1998), brinjal (Kumar et al, 1998), potato (Chakrabarti et al, 2000) and tomato (Mandaokar et al, 2000;Kumar and Kumar, 2004). Two chickpea cultivars (ICCV 1 and ICCV 6) have been transformed to express the cry1Ac gene under a constitutive promotor (CaMV35S) through microprojectile bombardment using the nptII gene as the selection marker (Kar et al, 1997).…”

Section: Helicoverpa Armigeramentioning

confidence: 99%

The potential of transgenic chickpeas for pest control and possible effects on non-target arthropods

Romeis

Sharma

et al. 2004

Crop Protection

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Chickpea, Cicer arietinum, is the third most important grain legume crop in the world, with India being the largest producer. Insect pests are a major constraint to chickpea production. In India, the legume pod borer Helicoverpa armigera is the major insect pest of chickpeas. However, sap-sucking insects that act as vectors for viral diseases and bruchid beetles in storage are also considered important pests. Here we give an overview over the different management options to control these pests. There is a growing interest in the genetic modification of crops to enhance their resistance against insect pests. Here we present the state-of-theart of chickpea transformation and give an overview on the available insecticidal genes that could be deployed to increase insect resistance in chickpea. Prior to commercialization, transgenic crops have to be assessed for their effects on the environment including the possible impact on non-target arthropods, many of which are important for biological pest control. Therefore, the arthropod-food web in the Indian chickpea system is described. Possible routes through which entomophagous insects could be exposed to insecticidal proteins expressed by genetically modified chickpeas are discussed, and species that could be selected for prerelease risk assessment are recommended.

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Section: Helicoverpa Armigeramentioning

confidence: 99%

The potential of transgenic chickpeas for pest control and possible effects on non-target arthropods

Romeis

Sharma

et al. 2004

Crop Protection

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“…A number of polyphagous insects, among them H. zea, a common pest of many Solanaceous plants such as the potato (4), have adapted to the protease inhibitors of their various host plants. Their survival and larval development is not affected by the presence of such molecules in the diet, because of their ability to express specific inhibitor-insensitive digestive proteolytic enzymes depending on the specific inhibitor repertoire encountered in the host plant which they colonize (5,6).…”

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confidence: 99%

Structural basis of the resistance of an insect carboxypeptidase to plant protease inhibitors

Bayés

Comellas-Bigler

Vega

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Corn earworm (Helicoverpa zea), also called tomato fruitworm, is a common pest of many Solanaceous plants. This insect is known to adapt to the ingestion of plant serine protease inhibitors by using digestive proteases that are insensitive to inhibition. We have now identified a B-type carboxypeptidase of H. zea (CPBHz) insensitive to potato carboxypeptidase inhibitor (PCI) in corn earworm. To elucidate the structural features leading to the adaptation of the insect enzyme, the crystal structure of the recombinant CPBHz protein was determined by x-ray diffraction. CPBHz is a member of the A͞B subfamily of metallocarboxypeptidases, which displays the characteristic metallocarboxypeptidase ␣͞␤-hydrolase fold, and does not differ essentially from the previously described Helicoverpa armigera CPA, which is very sensitive to PCI. The data provide structural insight into several functional properties of CPBHz. The high selectivity shown by CPBHz for C-terminal lysine residues is due to residue changes in the S1 substrate specificity pocket that render it unable to accommodate the side chain of an arginine. The insensitivity of CPBHz to plant inhibitors is explained by the exceptional positioning of two of the main regions that stabilize other carboxypeptidase-PCI complexes, the ␤8-␣9 loop, and ␣7 together with the ␣7-␣8 loop. The rearrangement of these two regions leads to a displacement of the active-site entrance that impairs the proper interaction with PCI. This report explains a crystal structure of an insect protease and its adaptation to defensive plant protease inhibitors.crystal structure ͉ inhibitor resistance ͉ Helicoverpa zea ͉ metallocarboxypeptidase T he larvae of plant-eating insects have a high need for free amino acids and nitrogen during their development. Normally, they meet these requirements by degrading dietary proteins. The digestive enzymes present in the larvae of the lepidopteran insects such as Helicoverpa zea are closely related to those secreted from the mammalian pancreas, comprising serine endopeptidases like trypsins and chymotrypsins and exopeptidases such as metallocarboxypeptidases and aminopeptidases (1, 2).Plants have evolved a defense that interferes with the insects' digestive system by expressing a number of different protease inhibitors (PIs), which are either constitutively present or strongly up-regulated in response to wound damage (3). Most PIs are small proteins that have been found in all plant species investigated thus far and occur in both reproductive and vegetative tissues. In herbivorous insects, they act by inhibiting protein digestive enzymes in the guts of insect larvae or adults, resulting in amino acid deficiencies that lead to serious developmental delay, mortality, or reduced fecundity.A number of polyphagous insects, among them H. zea, a common pest of many Solanaceous plants such as the potato (4), have adapted to the protease inhibitors of their various host plants. Their survival and larval development is not affected by the presence of such molecules in th...

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“…Various Bt transgenic potato lines have been developed that express cry genes and produce a considerable amount of Cry proteins that are effective enough for insect pest management under field conditions (Chakrabarti et al 2000, Ghasimi hag et al 2009, Mehrotra et al 2011. Laboratories in several countries have developed insect-resistant transformed potatoes; however, there is currently no commercial use of transgenic potatoes.…”

Section: H Salehian Et Almentioning

confidence: 99%

Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva

Salehian

Rahnama

Dezhsetan

et al. 2021

Crop Breed. Appl. Biotechnol.

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The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control.

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Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner)

Cited by 20 publications

References 21 publications

The potential of transgenic chickpeas for pest control and possible effects on non-target arthropods

The potential of transgenic chickpeas for pest control and possible effects on non-target arthropods

Structural basis of the resistance of an insect carboxypeptidase to plant protease inhibitors

Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva

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