Back-Flush Column-Switching Technique for on-Line Sample Cleanup and Enrichment to Determine Guaiphenesin in Human Serum

Emara, Samy; Kamal, Maha; Hadad, Ghada M.; Zaazaa, Hala E.; Kawi, Mohamed Abdel

doi:10.1080/10826076.2011.593226

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…The validated method was applied to the measurement of enantiomers of both GUA and KET in human plasma samples after being fortified with different concentrations of GUA and KET (Table ). These concentrations were selected to cover the expected levels in clinical samples . Since both GUA and KET are found in plasma as the intact drugs and their metabolites are only excreted in urine, this gives a great advantage to the direct application of our proposed method to the monitoring of the drug levels in human plasma following actual administration of the studied combination (GUA and KET) to human volunteers.…”

Section: Resultsmentioning

confidence: 99%

“…Since no change in response along with the absence of any degradation peaks was observed, the stability of GUA and KET prepared plasma samples for up to 24 h was proved. No long‐term stability studies were conducted here, as previous works have shown that KET and GUA have good stability in frozen samples.…”

Section: Resultsmentioning

confidence: 99%

“…) has expectorant properties and is widely used in cough‐remedy formulations. It is used for short‐term relief of dry nonproductive cough and mucus in the breathing passages . GUA is thought to act as an expectorant by increasing the volume and reducing the viscosity of secretions in the trachea and bronchi.…”

Section: Introductionmentioning

confidence: 99%

“…It is used for short-term relief of dry nonproductive cough and mucus in the breathing passages. 10,16,17 GUA is thought to act as an expectorant by increasing the volume and reducing the viscosity of secretions in the trachea and bronchi. Thus, it may increase the efficiency of the cough reflex and facilitate the removal of the secretions.…”

Section: Introductionmentioning

confidence: 99%

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Novel Stereoselective High‐Performance Liquid Chromatographic Method for Simultaneous Determination of Guaifenesin and Ketorolac Enantiomers in Human Plasma

et al. 2014

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A novel method was developed for the simultaneous determination of guaifenesin (GUA) and ketorolac tromethamine (KET) enantiomers in plasma samples. Since GUA probably increases the absorption of coadministered drugs (e.g., KET), it would be extremely important to monitor KET plasma levels for the purpose of dose adjustment with a subsequent decrease in the side effects. Enantiomeric resolution was achieved on a polysaccharide-based chiral stationary phase, amylose-2, as a chiral selector under the normal phase (NP) mode and using ornidazole (ORN) as internal standard. This innovative method has the advantage of the ease and reliability of sample preparation for plasma samples. Sample clean-up was based on simply using methanol for protein precipitation followed by direct extraction of drug residues using ethanol. Both GUA and KET enantiomers were separated using an isocratic mobile phase composed of hexane/isopropanol/trifluoroacetic acid, 85:15:0.05 v/v/v. Peak area ratios were linear over the range 0.05-20 µg/mL for the four enantiomers S (+) GUA, R (-) GUA, R (+) KET, and S (-) KET. The method was fully validated according to the International Conference on Harmonization (ICH) guidelines in terms of system suitability, specificity, accuracy, precision, robustness, and solution stability. Finally, this procedure was innovative to apply the rationale of developing a chiral high-performance liquid chromatography (HPLC) procedure for the simultaneous quantitative analysis of drug isomers in clinical samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Stereoselective High‐Performance Liquid Chromatographic Method for Simultaneous Determination of Guaifenesin and Ketorolac Enantiomers in Human Plasma

et al. 2014

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“…The technique of the column switching technique combined with LC–MS makes biopharmaceutical analysis automatic, rapid, and efficient, and it has been widely used in complex biological matrix analysis with serum 30 , blood 31 , plasma 32 , urine 33 , and other matrices.…”

Section: New Technology Of On-line Biological Sample Processingmentioning

confidence: 99%

New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

Peng

Tang

Zhou

et al. 2016

Acta Pharmaceutica Sinica B

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Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extraction, solid phase micro-extraction, column switching, limited intake filler, molecularly imprinted solid phase extraction, tubular column, and micro-dialysis. We describe new developments, principles, and characteristics of these techniques, and the application of liquid chromatography–mass spectrometry (LC–MS) in biopharmaceutical analysis with these new techniques in on-line biological sample processing.

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Recent advances in column switching high-performance liquid chromatography for bioanalysis

Cruz

Souza

Grecco

et al. 2021

Sustainable Chemistry and Pharmacy

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Back-Flush Column-Switching Technique for on-Line Sample Cleanup and Enrichment to Determine Guaiphenesin in Human Serum

Cited by 8 publications

References 11 publications

Novel Stereoselective High‐Performance Liquid Chromatographic Method for Simultaneous Determination of Guaifenesin and Ketorolac Enantiomers in Human Plasma

Novel Stereoselective High‐Performance Liquid Chromatographic Method for Simultaneous Determination of Guaifenesin and Ketorolac Enantiomers in Human Plasma

New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

Recent advances in column switching high-performance liquid chromatography for bioanalysis

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