Back from the Past: DNA Barcodes and Morphology Support Ablabesmyia americana Fittkau as a Valid Species (Diptera: Chironomidae)

Stur, Elisabeth; Silva, Fabio Laurindo da; Ekrem, Torbjørn

doi:10.3390/d11090173

Cited by 12 publications

(8 citation statements)

References 51 publications

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“…DNA barcodes have proven useful in differentiating chironomid species and providing novel insight into their taxonomy (Giłka et al., 2018; Lin et al., 2015; Lin et al., 2018a; Song et al, 2018; Stur et al., 2019). We show that DNA barcoding is an efficient tool for species delimitation and recognition of cryptic species in Tanytarsus .…”

Section: Discussionmentioning

confidence: 99%

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The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

et al. 2020

Diversity and Distributions

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Aim Reliable DNA barcode reference libraries are critical for biodiversity monitoring based on Environmental DNA (eDNA). DNA barcoding has proven successful in species delimitation and association of life stages in many groups of animals, such as non‐biting midges (Chironomidae). We use the genus Tanytarsus van der Wulp, 1874, second largest genus of the family Chironomidae, to test whether DNA barcodes can be used to identify unknown and cryptic species. A comprehensive DNA barcode library will be built to facilitate further eDNA studies for biodiversity monitoring and conservation in freshwater ecosystems. Location Mainland China. Methods In this study, the cytochrome c oxidase subunit 1 (COI) DNA barcodes from 298 individuals, representing 56 morphospecies from China, were analysed, including 136 sequences from previous studies and 162 newly generated DNA barcodes. 298 COI barcodes were applied into the Automatic Barcode Gap Discovery (ABGD) to compare the operational taxonomic units (OTU) number resulting from the Barcode Index Number (BIN) based “Barcode Gap Analysis” and neighbour joining (NJ) tree. Results Total 298 barcode sequences were applied to 66 BINs, including 20 singleton BINs and 46 concordant BINs. ABGD yielded 55 OTUs with a prior intraspecific divergence of Pmax = 3.59%. By Integrating DNA barcodes with morphology, we revealed 21 putative species new to science and two unidentified species from the 56 well‐separated morphospecies. Besides, several cryptic species are uncovered using the NJ tree and TCS haplotype network based on COI DNA barcodes. Main conclusions Our results demonstrate that DNA barcodes enable the discovery and identification of Tanytarsus non‐biting midges. A comprehensive DNA barcode reference library could speed up accurate species delimitation and discovery by providing a reliable resource for taxonomic studies, and improve the application of eDNA metabarcoding in biomonitoring of freshwater ecosystems.

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Section: Discussionmentioning

confidence: 99%

“…DNA barcodes have proven useful in differentiating chironomid species and providing novel insight into their taxonomy (Giłka et al, 2018;Lin et al, 2015;Lin et al, 2018a;Song et al, 2018;Stur et al, 2019).…”

Section: Taxonomymentioning

confidence: 99%

The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

et al. 2020

Diversity and Distributions

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“…Some sequences in the Lebertia diet that were previously identified only to family (Chironomidae) were similar enough to Ablabesmyia (approximately 9% pairwise distance) to be identified with that genus. Ablabesmyia is a genus found worldwide, with over 90 identified species [41]. In the Americas, they are primarily found in the Nearctic region, including the Laurentian Great Lakes.…”

Section: Improved Water Mite Diet Identificationsmentioning

confidence: 99%

“…Ablabesmyia annulata has the largest head capsule among 30 species of North American chironomids in which third instar larvae were compared [45]. Ablabesmyia is known to have a symbiotic relationship with freshwater mussels [46] and are predators of Tubifex tubifex, their own early instars, and other benthic macroinvertebrates [29,41,47,48]. Ablabesmyia mallochi is known to be more difficult to morphologically identify, so having an available barcode may assist in its identification in the future.…”

Section: Improved Water Mite Diet Identificationsmentioning

confidence: 99%

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

et al. 2022

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Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid species are present in the diets of diverse predators. Larval and adult chironomids from diverse habitats, including lakes, rivers, inland gardens, coastal vegetation, and nearshore habitats of the Great Lakes, were collected from 2012 to 2019. After morphological identification of chironomids, DNA was extracted and cytochrome oxidase I (COI) barcodes were PCR amplified and sequenced. Here we describe an analysis of biodiverse adult and larval chironomids in the Great Lakes region of North America based on new collections to improve chironomid identification by curating a chironomid DNA barcode database, thereby expanding the diversity and taxonomic specificity of DNA reference libraries for the Chironomidae family. In addition to reporting many novel chironomid DNA barcodes, we demonstrate here the use of this chironomid COI barcode database to improve the identification of DNA barcodes of prey in the liquefied diets of water mites. The species identifications of the COI barcodes of chironomids ingested by Lebertia davidcooki and L. quinquemaculosa are more diverse for L. davidcooki and include Parachironomus abortivus, Cryptochironomus ponderosus. Parachironomus tenuicaudatus, Glyptotendipes senilis, Dicrotendipes modestus, Chironomus riparius, Chironomus entis/plumosus, Chironomus maturus, Chironomus crassicaudatus, Endochironomus subtendens, Cricotopus sylvestris, Cricotopus festivellus, Orthocladius obumbratus, Tanypus punctipennis, Rheotanytarsus exiguus gr., and Paratanytarsus nr. bituberculatus.

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“…High success rates have been reported in some marine molluscs (Layton et al, 2014;van der Bank et al, 2013;Weigand et al, 2011) and other terrestrial molluscs but were not sufficient for some species (Chiba & Davison, 2008;Davison et al, 2009;Feng et al, 2011;Perez et al, 2014;van der Bank et al, 2013;Weigand et al, 2011). The absence of a DNA barcoding gap has been attributed to one or a combination of the following factors: incomplete sampling, misidentification of specimens, poor understanding of taxonomy, and the presence of cryptic diversity within taxa (Meyer & Paulay, 2005;Stur et al, 2019). These factors were evident in this study.…”

Section: Dna Barcoding Analysismentioning

confidence: 99%

Barcoding of South African forest‐dwelling snails (Mollusca: Gastropoda) reveals widespread cryptic diversity

Raphalo

Cole

Daniels

2021

Invertebrate Biology

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South Africa harbors a rich and diverse terrestrial snail fauna. Despite their importance in ecosystem functioning and structure, their alpha taxonomic diversity is poorly understood, and most groups have not been the subject of molecular systematic scrutiny. During the present study, we used the mitochondrial cytochrome c oxidase subunit I (COI) locus to start a DNA barcode reference library, to examine intra-and interspecific genetic divergence, and to explore the presence of cryptic diversity among terrestrial gastropods from forested regions in the country. We generated 399 COI barcodes comprising 70 morphospecies in 26 genera and 15 families, representing nearly 10% of the described gastropod diversity in South Africa. Our results showed a substantial overlap between mean intraspecific and interspecific genetic variation and no barcoding gap. Three species delimitation methods-Poisson Tree Processes (PTP), Refined Single Linkage (RESL) algorithm in Barcode of Life Data Systems, and the Automatic Barcode Gap Discovery (ABGD)-applied to selected widespread species retrieved incongruent operational taxonomic units (OTUs). These results, coupled with high intraspecific variation, suggested cryptic diversity among several terrestrial gastropod genera in the country. We further demonstrated that DNA barcoding analysis holds the potential for highlighting cryptic lineages in snails and for revealing taxa in need of taxonomic revision. We recommend that future taxonomic studies of South African terrestrial snails incorporate multiple sources of information for species delimitation.

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Back from the Past: DNA Barcodes and Morphology Support Ablabesmyia americana Fittkau as a Valid Species (Diptera: Chironomidae)

Cited by 12 publications

References 51 publications

The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

Barcoding of South African forest‐dwelling snails (Mollusca: Gastropoda) reveals widespread cryptic diversity

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