Background-free stimulated Raman scattering imaging by manipulating photons in the spectral domain

Zhu, Hanlin; Lee, Hyeon Jeong; Zhang, De‐Long

doi:10.1016/b978-0-323-85158-9.00002-6

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…not chemical specific, and spatially non-uniform in heterogeneous samples. Therefore, they don't provide any chemical information, while instead reducing the contrast, and creating artifacts in SRS imaging that could hinder interesting applications [41,57]. In the following, we describe in more detail the specific characteristics of these competing effects and how they lead to background signals in SRS microscopy.…”

Section: Background Signals In Im-srsmentioning

confidence: 99%

“…For this reason, SRS microscopy has been often referred to as a background-free CRS modality. However, there are other competing effects -in particular, cross-phase modulation (XPM), two-photon absorption (TPA), and thermal lensing (TL) -that generate ubiquitous and spatially uneven non-vibrationally resonant background signals in SRS measurements, reducing contrast and sensitivity [40,41]. Several applications of SRS microscopy would be hindered by the presence of those background signals, motivating the continuous development of novel strategies to minimize their contribution to the measured signal.…”

Section: Introductionmentioning

confidence: 99%

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Background signals in stimulated Raman scattering microscopy and current solutions to avoid them

Genchi

Laptenok

Liberale

2023

Advances in Physics: X

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Stimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant background that affects its analogous technique, coherent anti-Stokes Raman scattering. However, SRS is not a background-free technique. In fact, there are other optical processes -nonlinear transient scattering and nonlinear transient absorption -that can be detrimental to the contrast and sensitivity of SRS microscopy. In this review, we provide a description of these competing optical processes and present an up-to-date description of current solutions to minimize their effect on SRS measurements.

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Section: Background Signals In Im-srsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Background signals in stimulated Raman scattering microscopy and current solutions to avoid them

Genchi

Laptenok

Liberale

2023

Advances in Physics: X

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Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology

et al. 2022

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Spontaneous Raman microscopy reveals the chemical composition of a sample in a label-free and non-invasive fashion by directly measuring the vibrational spectra of molecules. However, its extremely low cross section prevents its application to fast imaging. Stimulated Raman scattering (SRS) amplifies the signal by several orders of magnitude thanks to the coherent nature of the nonlinear process, thus unlocking high-speed microscopy applications that provide analytical information to elucidate biochemical mechanisms with subcellular resolution. Nevertheless, in its standard implementation, narrowband SRS provides images at only one frequency at a time, which is not sufficient to distinguish constituents with overlapping Raman bands. Here, we report a broadband SRS microscope equipped with a home-built multichannel lock-in amplifier simultaneously measuring the SRS signal at 32 frequencies with integration time down to 44 µs, allowing for detailed, high spatial resolution mapping of spectrally congested samples. We demonstrate the capability of our microscope to differentiate the chemical constituents of heterogeneous samples by measuring the relative concentrations of different fatty acids in cultured hepatocytes at the single lipid droplet level and by differentiating tumor from peritumoral tissue in a preclinical mouse model of fibrosarcoma.

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Background-free stimulated Raman scattering imaging by manipulating photons in the spectral domain

Cited by 2 publications

References 38 publications

Background signals in stimulated Raman scattering microscopy and current solutions to avoid them

Background signals in stimulated Raman scattering microscopy and current solutions to avoid them

Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology

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