Bacopa monnieri extracts prevent hydrogen peroxide-induced oxidative damage in a cellular model of neuroblastoma IMR32 cells

Bhatia, Gaurav; Dhuna, Vikram; Dhuna, Kshitija; Kaur, Manpreet; Singh, Jatinder

doi:10.1016/s1875-5364(18)30017-7

Cited by 14 publications

(10 citation statements)

References 47 publications

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“…H 2 O 2 is the precursor of deleterious oxygen species which causes serious brain injury by producing ROS. Bhatia et al, [17] addressed the problem and performed an experiment, and examine the neuroprotective role of Bacopa monnieri extract (BME) against H 2 O 2 persuade oxidative stress using a cellular mode neuroblastoma IMR32 cell lines. Protective nature of methanolic ethanolic and water extract examined through MTT assay.…”

Section: Anti-oxidant Activitymentioning

confidence: 99%

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Pandey¹

2022

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Since the beginning of humankind, people are using numerous plants to cure diseases and abnormalities. Bacopa monnieri has been used by people for a long time. This plant has several medicinal and biochemical properties which would be beneficial to treat diseases. The current review article summarizes the medicinal, presence of the bioactive compound, mechanism of action, therapeutic importance, clinical trials, ethnobotanical usage, and distribution of the plant with respect to India and worldwide. Relevant information and literature on Bacopa monnieri were extracted from different sources and their finding were discussed in the manuscript. The number of therapeutic properties of the plant has been seen in the animal model experiment including anti-diarrheal, antioxidant, anti-inflammatory, anti-cancer, anti-fungal, anti-bacterial, anti-convulsant, hepatoprotective, antiulcer, anti-depressant, anti-hyperglycemic and anti-nociceptive activity, immunostimulatory activity, and wound healing activity. This article recapitulates ethnopharmacological uses of Bacopa monnieri to explore its therapeutic potentials thereby providing a basis for future research. Bacopa monnieri exhibits a comprehensive of pharmacological activities that could be observed by the presence of a vast range of chemical constituents. Manuscript also comprises tissue culture techniques and propagation of Bacopa monnieri. The study also provides a descriptive outlook on the uses of Bacopa monnieri in wastewater treatment. This review article highlights the endless possibilities present in the plant and also sheds light on research that has not yet been done on this plant and may prove useful if done in the future.

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Section: Anti-oxidant Activitymentioning

confidence: 99%

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Pandey¹

2022

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“…H 2 O 2 in medium mediates oxidative injury and inammation in vitro, thus simulating the tissue microenvironment aer nerve injury. [32][33][34] Our results showed that NSCs on the PLGA/GO nanobres had a better survival rate than PLGA alone, suggesting that GO was effective in preventing NSCs on the surface of the graphene-polymer hybrid scaffolds from reactive oxygen species (ROS)-mediated cell death. Previous research has demonstrated that GO protects mesenchymal stem cells (MSCs) and provides microenvironments suitable for long-term MSC survival in vivo and in vitro.…”

Section: Nsc Survival Ratementioning

confidence: 85%

Enhancement of neural stem cell survival, proliferation and differentiation by IGF-1 delivery in graphene oxide-incorporated PLGA electrospun nanofibrous mats

Guo

Zheng

et al. 2019

RSC Adv.

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“…Liver cells contains large amount of iron, which could transform H 2 O 2 reaction into hydroxyl radical (‐OH). Hydroxyl radical would attack DNA, protein and other macromolecules, further leads to DNA strand breakage, base exchange, chromosome variation, protein misfolding, and so on, and also attack the cell membrane (Bhatia, Dhuna, Dhuna, Kaur, & Singh, 2017; Nagao et al., 2018).…”

Section: Resultsmentioning

confidence: 99%

Garlic flavonoids alleviate H₂O₂ induced oxidative damage in L02 cells and induced apoptosis in HepG2 cells by Bcl‐2/Caspase pathway

Gao

Jia

Santhanam

et al. 2021

Journal of Food Science

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Liver damage is a common liver disorder, which could induce liver cancer. Oral antioxidant is one of the effective treatments to prevent and alleviate liver damage. In this study, three flavonoids namely myricetin, isoquercitrin, and isorhamnetin were isolated and identified from Laba garlic. The isolated compounds were investigated on the protective effects against H2O2‐induced oxidative damages in hepatic L02 cells and apoptosis inducing mechanism in hepatic cancer cells HepG2 by using MTT assay, flow cytometry and western blotting analysis. Myricetin, isoquercitrin, and isorhamnetin showed proliferation inhibition on HepG2 cells with IC50 value of 44.32 ± 0.213 µM, 49.68 ± 0.192 µM, and 54.32 ± 0.176 µM, respectively. While they showed low toxicity on normal cell lines L02. They could significantly alleviate the oxidative damage towards L02 cells (P < 0.05), via inhibiting the morphological changes in mitochondria and upholding the integrity of mitochondrial structure and function. The fluorescence intensity of L02 cells pre‐treated with myricetin, isoquercitrin, and isorhamnetin (100 µM) was 89.23 ± 1.26%, 89.35 ± 1.43% and 88.97 ± 0.79%, respectively. Moreover, the flavonoids could induce apoptosis in HepG2 cells via Bcl‐2/Caspase pathways, where it could up‐regulate the expression of Bax and down‐regulate the expression of Bcl‐2, Bcl‐xL, pro‐Caspase‐3, and pro‐Caspase‐9 proteins in a dose dependent manner. Overall, the results suggested that the flavonoids from Laba garlic might be a promising candidate for the treatment of various liver disorders.Practical ApplicationFlavonoids from Laba garlic showed selective toxicity towards HepG2 cells in comparison to L02 cells via regulating Bcl‐2/caspase pathway. Additionally, the isolated flavonoids expressively barred the oxidative damage induced by H2O2 in L02 cells. These results suggested that the flavonoids from laba garlic could be a promising agent towards the development of functional foods.

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Bacopa monnieri extracts prevent hydrogen peroxide-induced oxidative damage in a cellular model of neuroblastoma IMR32 cells

Cited by 14 publications

References 47 publications

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Enhancement of neural stem cell survival, proliferation and differentiation by IGF-1 delivery in graphene oxide-incorporated PLGA electrospun nanofibrous mats

Garlic flavonoids alleviate H₂O₂ induced oxidative damage in L02 cells and induced apoptosis in HepG2 cells by Bcl‐2/Caspase pathway

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Bacopa monnieri extracts prevent hydrogen peroxide-induced oxidative damage in a cellular model of neuroblastoma IMR32 cells

Cited by 14 publications

References 47 publications

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Bacopa monnieri (Linn.) Pennell - A Possible Plant for Impossible Diseases (A Review)

Enhancement of neural stem cell survival, proliferation and differentiation by IGF-1 delivery in graphene oxide-incorporated PLGA electrospun nanofibrous mats

Garlic flavonoids alleviate H2O2 induced oxidative damage in L02 cells and induced apoptosis in HepG2 cells by Bcl‐2/Caspase pathway

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Garlic flavonoids alleviate H₂O₂ induced oxidative damage in L02 cells and induced apoptosis in HepG2 cells by Bcl‐2/Caspase pathway