Bacteraemia and multiple liver abscesses due to<i>Fusobacterium nucleatum</i>in a patient with oropharyngeal malignancy

García-Carretero, Rafael

doi:10.1136/bcr-2018-228237

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Several studies have identified increased abundances of the oral microbe Fusobacterium in setting of colorectal cancer ( 29 , 33 – 35 ), liver cirrhosis ( 36 – 38 ), primary sclerosing cholangitis ( 39 – 41 ), gastroesophageal reflux disease ( 42 – 46 ), HIV infection ( 47 – 49 ), alcoholism ( 50 ), and IBD ( 6 , 10 – 19 ). Given the prevalence of F. nucleatum in mucosal specimens, we tested the hypothesis that this pathobiont could promote an epithelial proinflammatory response and potentially contribute to intestinal inflammation.…”

Section: Resultsmentioning

confidence: 99%

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Engevik

Danhof

Ruan

et al. 2021

mBio

153

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Multiple studies have implicated microbes in the development of inflammation, but the mechanisms remain unknown. Bacteria in the genus Fusobacterium have been identified in the intestinal mucosa of patients with digestive diseases; thus, we hypothesized that Fusobacterium nucleatum promotes intestinal inflammation. The addition of >50 kDa F. nucleatum conditioned media, which contain outer membrane vesicles (OMVs), to colonic epithelial cells stimulated secretion of the proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor (TNF). In addition, purified F. nucleatum OMVs, but not compounds <50 kDa, stimulated IL-8 and TNF production; which was decreased by pharmacological inhibition of Toll-like receptor 4 (TLR4). These effects were linked to downstream effectors p-ERK, p-CREB, and NF-κB. F. nucleatum >50-kDa compounds also stimulated TNF secretion, p-ERK, p-CREB, and NF-κB activation in human colonoid monolayers. In mice harboring a human microbiota, pretreatment with antibiotics and a single oral gavage of F. nucleatum resulted in inflammation. Compared to mice receiving vehicle control, mice treated with F. nucleatum showed disruption of the colonic architecture, with increased immune cell infiltration and depleted mucus layers. Analysis of mucosal gene expression revealed increased levels of proinflammatory cytokines (KC, TNF, IL-6, IFN-γ, and MCP-1) at day 3 and day 5 in F. nucleatum-treated mice compared to controls. These proinflammatory effects were absent in mice who received F. nucleatum without pretreatment with antibiotics, suggesting that an intact microbiome is protective against F. nucleatum-mediated immune responses. These data provide evidence that F. nucleatum promotes proinflammatory signaling cascades in the context of a depleted intestinal microbiome. IMPORTANCE Several studies have identified an increased abundance of Fusobacterium in the intestinal tracts of patients with colon cancer, liver cirrhosis, primary sclerosing cholangitis, gastroesophageal reflux disease, HIV infection, and alcoholism. However, the direct mechanism(s) of action of Fusobacterium on pathophysiological within the gastrointestinal tract is unclear. These studies have identified that F. nucleatum subsp. polymorphum releases outer membrane vesicles which activate TLR4 and NF-κB to stimulate proinflammatory signals in vitro. Using mice harboring a human microbiome, we demonstrate that F. nucleatum can promote inflammation, an effect which required antibiotic-mediated alterations in the gut microbiome. Collectively, these results suggest a mechanism by which F. nucleatum may contribute to intestinal inflammation.

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Section: Resultsmentioning

confidence: 99%

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Engevik

Danhof

Ruan

et al. 2021

mBio

153

View full text Add to dashboard Cite

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“… AF atrial fibrillation, HTN hypertension, CAD coronary artery disease, DM diabetes mellitus, NA not-applicable, S sensitive, N/T: Not-tested. Case 1, 3, 5, 7 [ 7 ], case 2 [ 20 ], case 4 [ 21 ], case 6 [ 22 ], case 8 [ 4 ], case 9 [ 23 ], case 10 [ 24 ], case 11 [ 25 ], case 12 [ 26 ], case 13 [ 5 ] …”

Section: Discussionmentioning

confidence: 99%

A case report of severe Fusobacterium nucleatum sepsis secondary to nephrectomy

et al. 2022

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Background Fusobacterium nucleatum (F. nucleatum) is a resident anaerobic bacterium, which in rare cases may invade blood from the head and neck or the digestive tract to cause bacteremia and induce venous thrombosis. F. nucleatum is closely related to abdominal tumors, but it has not been reported in relation to renal tumors. We report herein a possible case. Case presentation This patient had kidney cancer with thrombosis in the right renal vein but had no sign of infection. After radical nephrectomy, thrombi formed in his left renal vein, and when removed, severe sepsis occurred. He did not respond to treatment with antibiotics and died, but the blood culture done confirmed that he had F. nucleatum bacteremia. Conclusion F. nucleatum may also be associated with kidney cancer, and could cause post-operative renal vein thrombosis, and sepsis or septic shock after thrombectomy.

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Bacteraemia and multiple liver abscesses due toFusobacterium nucleatumin a patient with oropharyngeal malignancy

Cited by 2 publications

References 9 publications

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

A case report of severe Fusobacterium nucleatum sepsis secondary to nephrectomy

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