Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research

Hao, Mengling; Tang, Jingjing; Ge, Shengxiang; Li, Tingdong; Xia, Ningshao

doi:10.3390/microorganisms11030589

Cited by 3 publications

(2 citation statements)

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“…Gene editing technology refers to the artificial alteration of a specific gene in an organism to control its gene expression or function [3][4]. The implementation of modern artificial gene editing technology is based on the principle of DNA mutation.…”

Section: Gene Editingmentioning

confidence: 99%

Applications and Challenges of Artificial Intelligence in Life Sciences

Liu,

2024

SHS Web of Conf.

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With the continuous development of science and technology, exploration in the field of life sciences has become increasingly profound, encompassing the understanding of human beings themselves and the exploration of disease etiology and treatment methods.Artificial intelligence is defined as the intelligence exhibited by artificially created systems. The application of artificial intelligence is quite extensive and can replace humans in various aspects of work, significantly enhancing efficiency in daily life and work. Artificial intelligence has a dual nature, as it could provide convenience to human society but could also bring about various adverse effects.This paper summarizes the current applications of artificial intelligence in the field of life sciences. By analyzing the applications of artificial intelligence in areas such as gene editing, drug development, and bioinformatics, it demonstrates the potential of artificial intelligence in the field of life sciences. At the same time, this paper also points out the ethical, technical, social, and various challenges that the application of artificial intelligence technology in the field of life sciences faces, with the aim of providing reference for research in related fields.

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Section: Gene Editingmentioning

confidence: 99%

Applications and Challenges of Artificial Intelligence in Life Sciences

Liu,

2024

SHS Web of Conf.

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“…The development of bacterial artificial chromosome (BAC) technology has revolutionized the genetics of herpesviruses ( 11–15 ). Advances in BAC-based genome editing have been instrumental in helping us gain insight into herpesvirus gene function and vaccine development, thus furthering our knowledge of herpesviruses and paving the way for more effective vaccines and treatments (this is well reviewed by Xia and coworkers ( 16 )). Despite its advantages, BAC-based mutagenesis has some drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Genome editing of pseudorabies virus in the CRISPR/Cas9 era: a mini-review

et al. 2023

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Pseudorabies virus (PRV) is an important swine virus that has a significant impact on the global swine industry. PRV is a member of the herpesvirus family, specifically the alphaherpesvirus subfamily, and has been extensively utilized as a prototype herpesvirus. Notably, recent studies have reported that PRV sporadically spills over into humans. The PRV genome is approximately 150 kb in size and is difficult to manipulate at the genomic level. The development of clustered regularly interspaced short palindromic repeat-associated protein (CRISPR/Cas9) technology has revolutionized PRV genome editing. CRISPR/Cas9 has been widely used in the construction of reporter viruses, knock-out/knock-in of genes of interest, single virus tracking and antiviral strategies. Most importantly, for vaccine development, virulence gene knockout PRV vaccine candidates can be obtained within 2 weeks using CRISPR/Cas9. In this mini-review, we provide a concise overview of the application of CRISPR/Cas9 in PRV research and mainly share our experience with methods for efficiently editing the PRV genome. Through this review, we hope to give researchers better insight into the genome editing of pseudorabies virus.

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