Bacterial cellulose production, functionalization, and development of hybrid materials using synthetic biology

Vadanan, Sundaravadanam Vishnu; Basu, Anindya; Lim, Sierin

doi:10.1038/s41428-021-00606-8

Cited by 26 publications

(22 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Industrial processes in which Acetobacteraceae members of the acetic acid bacteria (AAB) group are exploited, include fermented beverages production, where BC is an undesired compound ( Giudici et al, 2009 ; Gullo et al, 2016 ). Within AAB, strains belonging to the Komagataeibacter genus are not only interesting for fermentation processes because of their high aptitude in producing organic acids ( Wang et al, 2015 ), but are widely studied for their ability in producing BC ( Gullo et al, 2018 ; La China et al, 2021 ; Vadanan et al, 2022 ). At least six species of Komagataeibacter were described as BC producers, namely K. europaeus, K. hansenii, K. rhaeticus, K. medellinensis, K. melomenusus, and K. xylinus .…”

Section: Introductionmentioning

confidence: 99%

Better under stress: Improving bacterial cellulose production by Komagataeibacter xylinus K2G30 (UMCC 2756) using adaptive laboratory evolution

et al. 2022

View full text Add to dashboard Cite

Among naturally produced polymers, bacterial cellulose is receiving enormous attention due to remarkable properties, making it suitable for a wide range of industrial applications. However, the low yield, the instability of microbial strains and the limited knowledge of the mechanisms regulating the metabolism of producer strains, limit the large-scale production of bacterial cellulose. In this study, Komagataeibacter xylinus K2G30 was adapted in mannitol based medium, a carbon source that is also available in agri-food wastes. K. xylinus K2G30 was continuously cultured by replacing glucose with mannitol (2% w/v) for 210 days. After a starting lag-phase, in which no changes were observed in the utilization of mannitol and in bacterial cellulose production (cycles 1–25), a constant improvement of the phenotypic performances was observed from cycle 26 to cycle 30, accompanied by an increase in mannitol consumption. At cycle 30, the end-point of the experiment, bacterial cellulose yield increased by 38% in comparision compared to cycle 1. Furthermore, considering the mannitol metabolic pathway, D-fructose is an intermediate in the bioconversion of mannitol to glucose. Based on this consideration, K. xylinus K2G30 was tested in fructose-based medium, obtaining the same trend of bacterial cellulose production observed in mannitol medium. The adaptive laboratory evolution approach used in this study was suitable for the phenotypic improvement of K. xylinus K2G30 in bacterial cellulose production. Metabolic versatility of the strain was confirmed by the increase in bacterial cellulose production from D-fructose-based medium. Moreover, the adaptation on mannitol did not occur at the expense of glucose, confirming the versatility of K2G30 in producing bacterial cellulose from different carbon sources. Results of this study contribute to the knowledge for designing new strategies, as an alternative to the genetic engineering approach, for bacterial cellulose production.

show abstract

Section: Introductionmentioning

confidence: 99%

Better under stress: Improving bacterial cellulose production by Komagataeibacter xylinus K2G30 (UMCC 2756) using adaptive laboratory evolution

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The known cellulose producers so far are the various genera of Gram-negative bacteria such as Achromobacter , Pseudomonas , Acetobacter , Salmonella , Azotobacter , and Rhizobium and the Gram-positive bacterium Sarcina ventriculi . Among all the above, Komagataeibacter (formerly known as Acetobacter and Gluconacetobacter ) xylinus ( K. xylinus ) has been popularly used as a model organism to produce BC This is due to the fact that K. xylinus is a non-pathogenic bacterium and has been successfully used for the scale up BC production to the commercial level [ 5 , 22 ]. Therefore, in an effort to gain an in-depth understanding of the cellulose biosynthetic pathway and its regulation, K. xylinus was predominantly investigated.…”

Section: Biosynthesis Structure and Characteristics Of Bcmentioning

confidence: 99%

“…Drying is the BC processing method mainly used to remove its water content, which is another factor that influences the degree of crystallinity of BC [ 5 ]. The commonly used drying methods include air-, oven-, vacuum-, and freeze-drying.…”

Section: Biosynthesis Structure and Characteristics Of Bcmentioning

confidence: 99%

“…Within these, BC is a natural polysaccharide-based polymer produced by specialist acetic acid-producing bacterial genera to facilitate host-bacterial interaction [ 4 ] and/or as a protective biofilm envelope under harsh conditions in nature [ 5 ]. BC is a secreted product with unique structural and mechanical properties, produced via well-controlled biochemical pathways involving various catalytic enzymes [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Cellulose-Based Blends and Composites: Versatile Biomaterials for Tissue Engineering Applications

Raut

Asare

Mohamed

et al. 2023

IJMS

View full text Add to dashboard Cite

Cellulose of bacterial origin, known as bacterial cellulose (BC), is one of the most versatile biomaterials that has a huge potential in tissue engineering due to its favourable mechanical properties, high hydrophilicity, crystallinity, and purity. Additional properties such as porous nano-fibrillar 3D structure and a high degree of polymerisation of BC mimic the properties of the native extracellular matrix (ECM), making it an excellent material for the fabrication of composite scaffolds suitable for cell growth and tissue development. Recently, the fabrication of BC-based scaffolds, including composites and blends with nanomaterials, and other biocompatible polymers has received particular attention owing to their desirable properties for tissue engineering. These have proven to be promising advanced materials in hard and soft tissue engineering. This review presents the latest state-of-the-art modified/functionalised BC-based composites and blends as advanced materials in tissue engineering. Their applicability as an ideal biomaterial in targeted tissue repair including bone, cartilage, vascular, skin, nerve, and cardiac tissue has been discussed. Additionally, this review briefly summarises the latest updates on the production strategies and characterisation of BC and its composites and blends. Finally, the challenges in the future development and the direction of future research are also discussed.

show abstract

“…Other advantages coming from BC structure are related to the possibility of biological, physical and chemical modification of the natural polymer membranes by different methods such as: i) surface treatment; ii) blending; iii) molecular modification; iv) modification of pore structure and v) polymer grafting (Choi and Shin 2020;Li et al 2017;Vadanan et al 2022;Zhijiang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Improved degradability and mechanical properties of bacterial cellulose grafted with PEG derivatives

et al. 2023

View full text Add to dashboard Cite

New functional materials based on bacterial cellulose (BC) grafted with poly(ethylene glycol) PEG derivatives for food packaging applications and a facile method for assessing the degradation rates of the final materials are presented. Two types of materials were obtained by grafting the BC films (BCF), respectively lyophilized BC pellicles (BCL) with three PEG derivatives of different molecular weights through radical polymerization. The BC based polymer materials were characterized by SEM, FT-IR, contact angle measurements, and TGA. Tensile tests and DMA analysis were used to compare the two types of materials in terms of shear-modulus, tensile strength and performance giving suitable information for food packaging applications. A new degradation evaluation method, that we propose herein, offers quantitative information about the degradation process in contrast with the SEM analysis, primarily used in literature, which is not decisive in all cases because it characterizes only small parts of the sample. The degradation rates evidenced that the PEG derivatives of higher molecular weight grafted on the surface of BCF led to an acceleration of the degradation process compared with the pristine samples. A good correlation was obtained between the samples analyzed by SEM after the degradation process and their degradation rates were mathematically determined.

show abstract

Bacterial cellulose production, functionalization, and development of hybrid materials using synthetic biology

Cited by 26 publications

References 111 publications

Better under stress: Improving bacterial cellulose production by Komagataeibacter xylinus K2G30 (UMCC 2756) using adaptive laboratory evolution

Better under stress: Improving bacterial cellulose production by Komagataeibacter xylinus K2G30 (UMCC 2756) using adaptive laboratory evolution

Bacterial Cellulose-Based Blends and Composites: Versatile Biomaterials for Tissue Engineering Applications

Improved degradability and mechanical properties of bacterial cellulose grafted with PEG derivatives

Contact Info

Product

Resources

About