Bacterial coaggregation: an integral process in the development of multi-species biofilms

Rickard, Alexander H.; Gilbert, Peter; High, Nicola J.; Kolenbrander, Paul E.; Handley, Pauline S.

doi:10.1016/s0966-842x(02)00034-3

Cited by 644 publications

(538 citation statements)

References 54 publications

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“…For instance, during colonization of the oral cavity, primary colonisers (such as Candida albicans) allow the attachment of other species (such as Streptococcus spp. ), facilitating the temporal co-aggregation of cells [103]. Should one of the primary colonisers be absent, co-aggregation will cease.…”

Section: In Vivo Investigation Of Biofilmsmentioning

confidence: 99%

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Roberts

Kragh

Bjarnsholt

et al. 2015

Journal of Molecular Biology

173

153

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We have become increasingly aware that during infection, pathogenic bacteria often grow in multicellular biofilms which are often highly resistant to antibacterial strategies. In order to understand how biofilms form and contribute to infection, in vitro biofilm systems such as microtitre plate assays and flow cells, have been heavily used by many research groups around the world. Whilst these methods have greatly increased our understanding of the biology of biofilms, it is becoming increasingly apparent that many of our in vitro methods do not accurately represent in vivo conditions. Here we present a systematic review of the most widely used in vitro biofilm systems, and we discuss why they are not always representative of the in vivo biofilms found in chronic infections. We present examples of methods that will help us to bridge the gap between in vitro and in vivo biofilm work, so that our bench-side data can ultimately be used to improve bedside treatment.

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Section: In Vivo Investigation Of Biofilmsmentioning

confidence: 99%

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Roberts

Kragh

Bjarnsholt

et al. 2015

Journal of Molecular Biology

173

153

View full text Add to dashboard Cite

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“…This process is affected by many factors, such as the type of surface, temperature, pH, physical interaction between the constituents, physicochemical characteristics of bacterial and spore surfaces, etc. (Brooks and Flint 2008;Rickard et al 2003). The presence of biofilms in equipment and tools of milk-processing plants, such as bends in pipes, gaskets, floors, milk handling devices, etc., has been well documented (Brooks and Flint 2008).…”

Section: Introductionmentioning

confidence: 99%

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Cherif-Antar

Moussa–Boudjemâa

Didouh

et al. 2015

Dairy Sci. & Technol.

105

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Bacteria may adhere to and develop biofilm structures onto dairy surfaces trying to protect themselves from adverse conditions such as pasteurization and CIP processes. Thus, biofilms are considered common sources of food contamination with undesirable bacteria. The purpose of this study was to evaluate the diversity of the microbiota attached to stainless steel surfaces in pre-and post-pasteurization pipe lines of a milk-processing plant. Seventy Gram-positive isolates were identified as Enterococcus faecalis (33), Bacillus cereus (26), Staphylococcus hominis (8), Staphylococcus saprophyticus (2), and Staphylococcus epidermidis-Staphylococcus aureus (1) species. Fifty-five Gram-negative isolates were identified to the species Escherichia coli (18), Klebsiella pneumoniae (13), Acinetobacter calcoaceticus (6), Serratia marcescens (6), Enterobacter spp. (5), Pseudomonas aeruginosa (4), Escherichia vulneris (2), and Proteus mirabilis (1). Fifty-five different strains were detected by the RAPD technique. These were subjected to an in vitro assay to evaluate their biofilm-forming capability. E. faecalis (7), A. calcoaceticus (4), K. pneumoniae (3), S. hominis (3), and P. aeruginosa (2) were the species in which more biofilm producer strains were encountered. The adhered microbiota was also assessed by the PCR-DGGE culture-independent technique. This analysis revealed a greater bacterial diversity than that revealed by culturing methods. In this way, in addition to the bacteria detected by culturing, DNA bands belonging to the genera Chrysobacterium and Streptomyces were also identified. This study emphasizes that knowledge of attached Dairy Sci. & Technol. (2016) 96:27-38 DOI 10.1007

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“…Alguns estudos têm sugerido que a produção de cápsula facilita a aderência bacteriana através de seus componentes biopolímeros, contribuindo para a formação de biofilme (Peters & Pulverer 1984). Sendo assim, os microorganismos que produzem biofilme são mais resistentes à ação de agentes físicos e químicos como os utilizados nos procedimentos de higienização (Rickard et al 2003, Marques 2005. A formação de biofilme é considerada como um dos principais fatores para infecção bacteriana persistente ou crônica (Costerton et al 1999).…”

Section: Discussionunclassified

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

et al. 2011

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Este estudo foi realizado com o objetivo de pesquisar Staphylococcus spp. de frangos de corte sadios e frangos de corte e poedeiras comerciais que apresentassem sinais clínicos respiratórios. Foram colhidos swabs dos seios infra-orbitários de 55 frangos de corte sadios, 35 com sinais respiratórios, e 30 poedeiras comerciais também com sinais respiratórios. Cada amostra foi composta por um "pool" de cinco aves, totalizando 24 amostras coletadas de 24 granjas comerciais. Para o isolamento foi utilizado o exame bacteriológico, com posterior avaliação das características morfológicas, tintoriais e bioquímica para determinação da espécie. Verificou-se a produção de hemólise, formação de biofilme em ágar Vermelho Congo (ACR), detecção do gene mecA pela PCR e avaliação da suscetibilidade a 13 drogas antimicrobianas. Das 24 amostras processadas, foram isolados 16 Staphylococcus, cinco isolados foram coagulase-positiva (SCP) e 11 coagulase-negativa (SCN), e nos testes de hemólise e formação de biofilme, três isolados apresentaram-se hemolíticos e seis foram positivos, respectivamente. Na avaliação por meio da PCR, para detecção do gene mecA todos os isolados apresentaram resultados negativo. Observaram-se que 15 isolados foram resistentes a cinco ou mais antibióticos, e que as drogas associadas apresentaram melhor perfil de sensibilidade. A resistência a antimicrobianos e cepas produtoras de biofilme podem interferir na resposta terapêutica de aves que apresentam sinais clínicos.

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Bacterial coaggregation: an integral process in the development of multi-species biofilms

Cited by 644 publications

References 54 publications

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

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