Bacterial Colonisation and Antibiotic Sensitivity Profile of Endotracheal Tubes in Mechanically Ventilated Patients

Hemanth, N; Kondagadapu, Swarnalatha; Kadiyala, Vinay; Chaudhury, Abhijit; Samantaray, Aloka

doi:10.7860/jcdr/2019/37402.12457

Cited by 5 publications

(6 citation statements)

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“…In a study conducted by Dutta et al, Shukla et al and Siddiqui et al, B. cepacia showed maximum susceptibility towards minocycline and cotrimoxazole. [23,24,25] Kady et al reported B. cepacia isolates to be 100% susceptible to meropenem, ceftazidime, cotrimoxazole, and minocycline. [26] In the present study, 23 The results obtained in the present study are concordant to Baidya et al, Rewdiwala et al and Kaur et al…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Kaur,

Nazir Reshi

2024

IJMRA

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Biofilm formation has been found to play a central role in many human infections in which antibiotic resistance is common. Bacteria can colonize and form biofilms on almost any surface, including natural and synthetic surfaces the present study was conducted with an aim to detect biofilm formation in ET tubes/ ET secretions received from ICU patients by microtiter plate method. In the present study a total of 85 isolates were obtained out of 400 samples. Among 85 isolates of gram- negative bacteria, maximum number of isolates were of K. pneumoniae (41.17%) followed by A. baumannii (40%), P. aeruginosa (10.5%), E. coli (5.88%) and B.cepacia (2.35%). All isolates were tested for antimicrobial sensitivity/resistance by Vitek 2 Compact system. All the isolates were further tested for biofilm production by microtiter plate method and optical density (OD value) was measured. Biofilm production was distributed by strong, medium, and weak. It is concluded that stringent hygiene protocols, routine tube changes and the use of antimicrobial coatings are crucial for prevention of biofilm development in ICU settings.

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Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Kaur,

Nazir Reshi

2024

IJMRA

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“…The ET’s distal part (approximately 3–5 cm) will be collected according to recommended techniques [ 45 , 47 ]. Subsequently, the sample will be divided with a sterile scissors into 4 pieces, 2 of which will be used for microbiological processing and 2 for gene sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…Since there is no standardized method used for the detection and identification of UC and ET biofilms, the investigators will utilize adapted techniques described in previous publications. ET and UC samples will be processed by the roll-plate technique and the rolled fragments will be placed on the medium surface for 48–72 h incubation at 37 °C ( Figure 3 and Figure 4 ) [ 45 , 47 , 48 , 51 ].…”

Section: Methodsmentioning

confidence: 99%

Indwelling Device-Associated Biofilms in Critically Ill Cancer Patients—Study Protocol

et al. 2021

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Health care-associated infections are a leading cause of inpatient complications. Rapid pathogen detection/identification is a major challenge in sepsis management that highly influences the successful outcome. The current standard of microorganism identification relies on bacterial growth in culture, which has several limitations. Gene sequencing research has developed culture-independent techniques for microorganism identification, with the aim to improve etiological diagnosis and, therefore, to change sepsis outcome. A prospective, observational, non-interventional, single-center study was designed that assesses biofilm-associated pathogens in a specific subpopulation of septic critically ill cancer patients. Indwelling device samples will be collected in septic patients at the moment of the removal of the arterial catheter, central venous catheter, endotracheal tube and urinary catheter. Concomitantly, clinical data regarding 4 sites (nasal, pharyngeal, rectal and skin) of pathogen colonization at the time of hospital/intensive care admission will be collected. The present study aims to offer new insights into biofilm-associated infections and to evaluate the infection caused by catheter-specific and patient-specific biofilm-associated pathogens in association with the extent of colonization. The analysis relies on the two following detection/identification techniques: standard microbiological method and next generation sequencing (NGS). Retrospectively, the study will estimate the clinical value of the NGS-based detection and its virtual potential in changing patient management and outcome, notably in the subjects with missing sepsis source or lack of response to anti-infective treatment.

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“…Malacarne et al ( 183 ) reported 28.6% of the cases of late-onset VAP due to A. baumannii which were preceded by tracheobronchial colonization with A. baumannii . The formation of biofilm on ETT and its association with VAP was analyzed in several studies where the most frequent bacteria were A. baumannii which lead to high mortality of patients ( 175 , 184 – 189 ).…”

Section: A Baumannii Sneaks Into Hospitals: Adult and Paedia...mentioning

confidence: 99%

Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection

et al. 2022

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Acinetobacter baumannii (A. baumannii) is a leading cause of nosocomial infections as this pathogen has certain attributes that facilitate the subversion of natural defenses of the human body. A. baumannii acquires antibiotic resistance determinants easily and can thrive on both biotic and abiotic surfaces. Different resistance mechanisms or determinants, both transmissible and non-transmissible, have aided in this victory over antibiotics. In addition, the propensity to form biofilms (communities of organism attached to a surface) allows the organism to persist in hospitals on various medical surfaces (cardiac valves, artificial joints, catheters, endotracheal tubes, and ventilators) and also evade antibiotics simply by shielding the bacteria and increasing its ability to acquire foreign genetic material through lateral gene transfer. The biofilm formation rate in A. baumannii is higher than in other species. Recent research has shown how A. baumannii biofilm-forming capacity exerts its effect on resistance phenotypes, development of resistome, and dissemination of resistance genes within biofilms by conjugation or transformation, thereby making biofilm a hotspot for genetic exchange. Various genes control the formation of A. baumannii biofilms and a beneficial relationship between biofilm formation and “antimicrobial resistance” (AMR) exists in the organism. This review discusses these various attributes of the organism that act independently or synergistically to cause hospital infections. Evolution of AMR in A. baumannii, resistance mechanisms including both transmissible (hydrolyzing enzymes) and non-transmissible (efflux pumps and chromosomal mutations) are presented. Intrinsic factors [biofilm-associated protein, outer membrane protein A, chaperon-usher pilus, iron uptake mechanism, poly-β-(1, 6)-N-acetyl glucosamine, BfmS/BfmR two-component system, PER-1, quorum sensing] involved in biofilm production, extrinsic factors (surface property, growth temperature, growth medium) associated with the process, the impact of biofilms on high antimicrobial tolerance and regulation of the process, gene transfer within the biofilm, are elaborated. The infections associated with colonization of A. baumannii on medical devices are discussed. Each important device-related infection is dealt with and both adult and pediatric studies are separately mentioned. Furthermore, the strategies of preventing A. baumannii biofilms with antibiotic combinations, quorum sensing quenchers, natural products, efflux pump inhibitors, antimicrobial peptides, nanoparticles, and phage therapy are enumerated.

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Bacterial Colonisation and Antibiotic Sensitivity Profile of Endotracheal Tubes in Mechanically Ventilated Patients

Cited by 5 publications

References 0 publications

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Indwelling Device-Associated Biofilms in Critically Ill Cancer Patients—Study Protocol

Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection

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