Bacterial communities in predatory mites are associated with species and diet types

Yan, Jun; Zhang, Bo; Li, Guiting; Xu, Xuenong

doi:10.1007/s10526-021-10112-8

Cited by 6 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We selected 100 gravid adults of each of the above 5 predatory mites to lay eggs in small arenas (Yan et al. , 2021). Approximately 200 eggs were collected for each species within 24 h. To reduce maternal and environmental effects, we disinfected the surface of predatory mite eggs by soaking in 0.5% sodium hypochlorite for 1 min and rinsing with sterile water 3 times.…”

Section: Methodsmentioning

confidence: 99%

“…The total DNA from each pooled sample was extracted using the Qiagen DNeasy Blood & Tissue Kit (Cat#69506). The V4 region of the bacterial 16S rRNA gene was amplified with the primers 515F (5 -GTGCCAGCMGCCGCGG-3 ) and 806R (5 -GGACTACHVGGGTWTCTAAT-3 ) using DNA Polymerase (Phusion HF DNA Polymerase M0530S, NEB) under the following procedure: 98 °C for 30 s; 35 cycles of 98 °C for 10 s, 55 °C for 20 s, and 72 °C for 15 s; and 72 °C for 5 min (Yan et al, 2021). Polymerase chain reaction (PCR) products were then purified using the Omega Bio-tek E.Z.N.A Cycle-Pure Kit (REF#D6492-02).…”

Section: Total Dna Extraction and High-throughput Sequencingmentioning

confidence: 99%

“…Due to their tiny body size, simple structure of their guts, and fast transition of ingested substances, predatory mites are considered to change their microbome frequently in unpredictable environments [34]. We have already revealed that bacterial community is determined by both species and diet in two predatory mites [47], however, to our knowledge, little research has been undertaken on microbiota in response to diet width in predatory mites.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Both host and diet shape bacterial communities of predatory mites

et al. 2023

Self Cite

View full text Add to dashboard Cite

Microbial communities, derived from food, ambient, and inner, can affect host ecological adaption and evolution. Comparing with most phytophagous arthropods, predators may have more opportunities to develop specific microbiota depending on the level of prey specialization. To explore how diet sources affect host microbial communities and vary across predator species, we considered 3 types of predators from Phytoseiidae (Acari: Mesostigmata): polyphagous (Amblyseius orientalis Ehara, Neoseiulus barkeri Hughes, and Amblyseius swirskii Athias‐Henrio), oligophagous (Neoseiulus californicus McGregor), and monophagous (Phytoseiulus persimilis Athias‐Henriot) predatory mites. The polyphagous species were fed on 2 types of diets, natural prey and alternative prey. By using 16S rRNA sequencing, we found that diet was the main source of microbiota in predatory mites, while there was no clear pattern affected by prey specialization. Among 3 polyphagous predators, host species had a larger impact than prey on microbial composition. Unlike A. orientalis or N. barkeri which showed consistency in their microbiota, prey switching significantly affected β‐diversity of bacterial composition in A. swirskii, with 56% of the microbial alteration. In short, our results confirmed the substantial influence of diet on host microbial construction in predatory species, and highlighted species differences in shaping the microbiota which are not necessarily related to prey specialization.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Total Dna Extraction and High-throughput Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Both host and diet shape bacterial communities of predatory mites

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adequate numbers of adult females of A. orientalis, N. californicus and N. barkeri were placed in small arenas with only prey T. urticae for 24 h to lay eggs (Yan et al 2021). Brie y, the arena was a tiny device with four tightly clipped layers: a transparent acrylic board (30×20×3 mm 3 ) with a 10 mm diameter hole in the middle, bean leaf disc and two pieces of rectangular glass (30×20×1 mm 3 ) each at top and bottom.…”

Section: Effects Of Heat Stresses On Phytoseiid Eggsmentioning

confidence: 99%

Effects of heat stresses on fitness of three commercial predatory mites

Hao,

Wang,

Yan

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

To explore the performances of predatory mites under high temperature, we selected three predatory mites Amblyseius orientalis, Neoseiulus californicus and Neoseiulus barkeri to investigate the survival rate, development duration and fecundity after their eggs and female adults were treated with high temperatures. The results showed that both eggs and adults of N. barkeri were most tolerant to heat stress than N. californicus and A. orientalis. After heat treatment of 39°C on egg, three predatory mites all presented shorter developmental period and longevity as well as lower fecundity. The oviposition period of N. californicus was shortened by nearly 50% and the total egg production of A. orientalis decreased by about 40%. However, the N. barkeri was the least affected by high temperature with its fecundity reduced by only 30%. The short-term high temperature treatment at adult stage also had negative effect on the oviposition period and longevity. The oviposition period of A. orientalis was reduced from 18.26 days to 3.82 days at 39℃, and 100% mortality occurred at 40°C. The longevity of N. californicus and N. barkeri suffered at 40℃ was shortened by about 50%. Our study confirmed that N. barkeri is tolerant to high temperatures at some extent, while A. orientalis is more heat sensitive in either two life stage. In sum, the understanding of the heat resistance of commercial predatory mites can improve the better application of biocontrol agents when selecting mite species for pest mite control in the field.

show abstract

“…The total DNA from each pooled sample was extracted using the Qiagen DNeasy Blood & Tissue Kit (Cat#69506). The V4 region of the bacterial 16S rRNA gene was ampli ed with the primers 515F (5'-GTGCCAGCMGCCGCGG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') using DNA Polymerase (Phusion HF DNA Polymerase M0530S, NEB) under the following procedure: 98 ℃ for 30 s; 35 cycles of 98 ℃ for 10 s, 55 ℃ for 20 s, and 72 ℃ for 15 s; and 72 ℃ for 5 min [47]. PCR products were then puri ed using the Omega Bio-tek E.Z.N.A Cycle-Pure Kit (REF#D6492-02).…”

Section: Total Dna Extraction and High-throughput Sequencingmentioning

confidence: 99%

Both host and diet shape bacterial communities of predatory mites

Yan

Wang

Wei

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Microbial communities, derived from food, ambient and inner, can affect host ecological adaption and evolution. Comparing with most of phytophagous arthropods, the diet of predators is pretty complex and varied. At a higher trophic level, there may be more opportunity for predators to develop specific microbiota which may also depend on the level of prey host specialization shown by the predators. To explore how diet sources affect host microbial communities and vary across predators species, we considered three types predators from the family of Phytoseiidae: polyphagous (Amblyseius orientalis Ehara, Neoseiulus barkeri Hughes and Amblyseius swirskii Athias-Henrio), oligophagous (Neoseiulus californicus McGregor), and monophagous (Phytoseiulus persimilis Athias-Henriot) predatory mites. The polyphagous species were fed on two types of diets, natural prey (Tetranychus urticae Koch) and alternative prey (Carpoglyphus lactis Linnaeus). By using 16S rRNA sequencing, we found that diet was the main source of microbiota in predatory mites, and that there was no clear effect of prey specialization. For the polyphagous mites, species had a larger impact than prey type on microbial composition. Unlike A. orientalis or N. barkeri which showed consistency in their microbiota regardless of prey type, prey switching significantly affected β-diversity of bacterial composition in A. swirskii, with 56% of the microbiota changing. In short, our results confirmed the substantial influence of diet on host microbial construction in predatory species but also highlighted species differences in shaping the microbiota which are not necessarily related to prey specialization.

show abstract

Bacterial communities in predatory mites are associated with species and diet types

Cited by 6 publications

References 45 publications

Both host and diet shape bacterial communities of predatory mites

Both host and diet shape bacterial communities of predatory mites

Effects of heat stresses on fitness of three commercial predatory mites

Both host and diet shape bacterial communities of predatory mites

Contact Info

Product

Resources

About