Bacterial community response in Juye mining area at the early stage of cracks

Abstract

Due to the sensitivity of microorganisms to the environment, microorganisms with strong tolerance in the early stage of coal mining collapse will gradually move to a dominant position, and plants can improve soil quality and provide important carbon sources for microorganisms. The soil characteristics and the response of soil bacteria in the early cracks during 15 ~ 20 days of mining were studied. Compared with non-cracked farmland group (C), soil bulk density in cracked farmland group (F) increased significantly in the early stage of coal mining,while porosity was on the contrary (p < 0.05). The mineral elements (except Ca and Na) in F were significantly lower than those in cracked abandoned land group (A).The abundance of the microbial community might be more closely related to crop planting, while the evenness of the microbial community was more affected by cracks. Coal mining cracks make Proteobacteria enrich significantly, while crop planting is conducive to the enrichment of RB41 and Pir4_lineage. Soil moisture content and AN were significantly negatively correlated with the relative abundance, while pH was significantly positively correlated with it. Planctomycetes and Bacteroidetes, which were significantly enriched in the non-crack area, were significantly positively correlated with AP, while Thaumarchaeot was significantly positively correlated with Eh. The study provided a basis for improving the low ecological environment damage mining technology.