1995
DOI: 10.1128/jb.177.16.4779-4791.1995
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Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex

Abstract: DNA transfer by bacterial conjugation requires a mating pair formation (Mpf) system that specifies functions for establishing the physical contact between the donor and the recipient cell and for DNA transport across membranes. Plasmid RP4 (IncP␣) contains two transfer regions designated Tra1 and Tra2, both of which contribute to Mpf. Twelve components are essential for Mpf, TraF of Tra1 and 11 Tra2 proteins, TrbB, -C, -D, -E, -F, -G, -H, -I, -J, -K, and -L. The phenotype of defined mutants in each of the Tra2… Show more

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Cited by 118 publications
(179 citation statements)
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“…Eleven mpf components (trbB to trbL) and traF are required for IncP pilus formation in the absence of any DNA-processing factors (92), and these components are also required to establish conjugative junctions (181). The mpf system of RP4 was localized in the cell membrane (89) and was suggested to form a complex that connects the cytoplasmic and the outer membrane.…”
Section: Conjugative Transfer In Gram-negative Bacteria As a Paradigmmentioning
confidence: 99%
“…Eleven mpf components (trbB to trbL) and traF are required for IncP pilus formation in the absence of any DNA-processing factors (92), and these components are also required to establish conjugative junctions (181). The mpf system of RP4 was localized in the cell membrane (89) and was suggested to form a complex that connects the cytoplasmic and the outer membrane.…”
Section: Conjugative Transfer In Gram-negative Bacteria As a Paradigmmentioning
confidence: 99%
“…95,[99][100][101] In the E. coli RP4 plasmid system, the mating channel is composed of 10 mating-pair gene products, a TraF pilin support protein and the coupling protein TraG. 95,102 In E. coli F plasmid system, the channel is composed of 11 proteins including the coupling protein TraD. 103 In A. tumefaciens, each of 12 proteins named VirB1 to VirB11 and VirD4 has been extensively characterized.…”
mentioning
confidence: 99%
“…By electron microscopy, IncP pili are seen in bundles, and often appear to be shed from the bacterial surface (32). These observations suggest a new possibility for the mating bridge, comprised of TraF, TraG, and a basal pilus structure perhaps created after the pilus has been shed or broken off from the cell surface.…”
Section: Donor Bacterium and Recipient Cell Contact And Mating Bridgementioning
confidence: 82%
“…oriT RK2 -containing mobilizable plasmids have been studied in regard to the mating bridge in E. coli (32). By Southern blot hybridization using an oriT sequence probe, the oriT has been localized to the inner membrane and not to the outer membrane (33).…”
Section: Donor Bacterium and Recipient Cell Contact And Mating Bridgementioning
confidence: 99%
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