Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China

Yang, Yu; Wan, Minxi; Shi, Wu-yang; Peng, Hong; Qiu, Guanzhou; Zhou, Jizhong; Liu, Xueduan

doi:10.3354/ame047141

Cited by 32 publications

(11 citation statements)

References 47 publications

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“…Nearly all the sequences identified as Rhodospirillales fell within the genus Acidiphilium, a heterotrophic iron-reducing Alphaproteobacterium. This taxon is commonly found in AMD sites, including the Dabaoshan Mine, China (26), and the Rio Tinto watershed (27). The most abundant order at Scalp Level Run was the Ferrovales.…”

Section: Discussionmentioning

confidence: 99%

Efficient Low-pH Iron Removal by a Microbial Iron Oxide Mound Ecosystem at Scalp Level Run

Grettenberger

Pearce

Bibby

et al. 2017

Appl Environ Microbiol

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Acid mine drainage (AMD) is a major environmental problem affecting tens of thousands of kilometers of waterways worldwide. Passive bioremediation of AMD relies on microbial communities to oxidize and remove iron from the system; however, iron oxidation rates in AMD environments are highly variable among sites. At Scalp Level Run (Cambria County, PA), first-order iron oxidation rates are 10 times greater than at other coal-associated iron mounds in the Appalachians. We examined the bacterial community at Scalp Level Run to determine whether a unique community is responsible for the rapid iron oxidation rate. Despite strong geochemical gradients, including a Ͼ10-fold change in the concentration of ferrous iron from 57.3 mg/liter at the emergence to 2.5 mg/liter at the base of the coal tailings pile, the bacterial community composition was nearly constant with distance from the spring outflow. Scalp Level Run contains many of the same taxa present in other AMD sites, but the community is dominated by two strains of Ferrovum myxofaciens, a species that is associated with high rates of Fe(II) oxidation in laboratory studies.IMPORTANCE Acid mine drainage pollutes more than 19,300 km of rivers and streams and 72,000 ha of lakes worldwide. Remediation is frequently ineffective and costly, upwards of $100 billion globally and nearly $5 billion in Pennsylvania alone. Microbial Fe(II) oxidation is more efficient than abiotic Fe(II) oxidation at low pH (P. C. Singer and W. Stumm, Science 167:1121-1123, 1970, https://doi.org/10.1126/ science.167.3921.1121). Therefore, AMD bioremediation could harness microbial Fe(II) oxidation to fuel more-cost-effective treatments. Advances will require a deeper understanding of the ecology of Fe(II)-oxidizing microbial communities and the factors that control their distribution and rates of Fe(II) oxidation. We investigated bacterial communities that inhabit an AMD site with rapid Fe(II) oxidation and found that they were dominated by two operational taxonomic units (OTUs) of Ferrovum myxofaciens, a taxon associated with high laboratory rates of iron oxidation. This research represents a step forward in identifying taxa that can be used to enhance costeffective AMD bioremediation.KEYWORDS Ferrovum myxofaciens, acid mine drainage, environmental microbiology, extremophiles, iron oxidation A cid mine drainage (AMD) is a devastating and widespread industrial pollution problem (1-6). The effects of AMD are felt especially hard in the Appalachian Plateau in the eastern United States, where over 8,000 km of streams are polluted by

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Section: Discussionmentioning

confidence: 99%

Efficient Low-pH Iron Removal by a Microbial Iron Oxide Mound Ecosystem at Scalp Level Run

Grettenberger

Pearce

Bibby

et al. 2017

Appl Environ Microbiol

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“…It is notable that the concentration of ferrous ions in Sample LC greatly exceeded those in Samples JX and FS. Ferrous ions are a key energy substance for many bacterial species [16]. Based on the results of the PCA analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

Archaeal diversity in acid mine drainage from Dabaoshan Mine, China

Qiu

Wan

Lin

et al. 2008

J. Basic Microbiol.

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Three acid mine drainage (AMD) samples collected from Dabaoshan Mine (Guangdong Province, China) were studied. In addition to physicochemical analyses, the diversity and community structures of the archaeal communities in these samples were described at the genetic level by amplified ribosomal DNA restriction analysis (ARDRA). Nine different ARDRA patterns were obtained from 146 clones and were studied as operational taxonomic units (OTUs), which were re-amplified and sequenced. Sequence data and phylogenetic analysis showed that most of the clones belonged to the Thermoplasmatales, and that archaea belonging to the Sulfolobales were absent. Only 1 OTU attributed to Ferroplasma was found and was observed to be abundant in all 3 samples. Eight OTUs were related to 2 new undefined groups in the Thermoplasmatales. Of the 8 OTUs, the clones in 2 similar units were isolated from samples collected from an abandoned sulfide mine (Huelva, Spain) and those in 5 similar units were isolated from samples collected from a closed copper mine (Tonglushan, China). These diversities were characterized by the reciprocal of Simpson's index (1/D) and correlated with the concentrations of ferrous ions and toxic ions in the AMD samples. The high temperature of the sampling sites was one of the factors that could explain why archaea belonging to the Thermoplasmatales were abundant in the analyzed AMD samples while those belonging to the Sulfolobales were absent.

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“…As reported by Yang et al (2007) and Auld et al (2013) the genus Acidiphilium is one of the dominants in acid mine drainage, and has been detected by both culture-dependent and independent methods. The species A. cryptum has been frequently cultivated from mining environments, being reported from a coal mine lake in Germany (Küsel et al, 1999), in a Norwegian cooper mine (Johnson et al, 2001) and from AMD from Chinese lead and zinc tailings (Tan et al, 2007), for instance.…”

Section: Discussionmentioning

confidence: 77%

Characterization of acidophilic bacteria related to Acidiphilium cryptum from a coal-mining-impacted river of South Brazil

Delabary¹,

Lima²,

Silva³

2017

Braz. J. Aquat. Sci. Technol.

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Three acidophilic bacteria were isolated from water and sediment samples collected at a coal mining-impacted river, Rio Sangão, located in the city of Criciúma, Santa Catarina state, in southern Brazil. These microorganisms were isolated in acid media and were phylogenetic related to the species Acidiphilium cryptum by its 16S rRNA gene sequences, although they differ from this species in the assimilation of some carbon sources. The optimum and the maximum pH for growth of all strains were nearly 3.0 and 5.0-6.0, respectively. Two of the strains were slightly more acidophilic, with the minimum pH for growth of 2.0. All strains also tolerate the four tested metals (Ni, Zn, Cu and Se) at variable concentrations, with LAMA 1486 being the most metal-resistant strain. These bacteria may belong to different ecotypes of A. cryptum, or even represent new species in the genus. Besides they bear characteristics that make them useful in the development of bioremediation process, for the treatment of sites contaminated with multiple toxic metals, including coal mining drainage.

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Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China

Cited by 32 publications

References 47 publications

Efficient Low-pH Iron Removal by a Microbial Iron Oxide Mound Ecosystem at Scalp Level Run

Efficient Low-pH Iron Removal by a Microbial Iron Oxide Mound Ecosystem at Scalp Level Run

Archaeal diversity in acid mine drainage from Dabaoshan Mine, China

Characterization of acidophilic bacteria related to Acidiphilium cryptum from a coal-mining-impacted river of South Brazil

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