Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil

Festa, Sabrina; Coppotelli, Bibiana Marina; Morelli, Irma Susana

doi:10.1016/j.ibiod.2013.06.006

Cited by 46 publications

(52 citation statements)

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“…From a chronically PAH-contaminated soil from Mosconi neighborhood (coordinates S 34° 52´31´ W 57° 55´10´) near La Plata City, Argentina (no specific permissions were required for this field location because it did not involve endangered or protected species), a phenanthrene-degrading bacterial consortium (CON) was obtained by culture enrichment technique in LMM with 2000 mg l -1 of phenanthrene as detailed in previous research [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…In previous work, a natural phenanthrene-degrading consortium (CON) was isolated from a chronically contaminated soil [ 17 ] and it was characterized in terms of diversity by pyrosequencing 16S rRNA gene fragments from its total DNA. This analysis revealed the presence of seven bacterial orders: Sphingomonadales , Rhodospirillales , Rhizobiales , Xanthomonadales , Pseudomonadales , Enterobacteriales and Burkholderiales [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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“…Complete mineralization tends to be slow (Wang et al, 2008a), or cannot be achieved by a single strain, because of the accumulation of toxic intermediates (Tixier et al, 2002;Festa et al, 2013). Under these circumstances, mineralization is better achieved by consortia (Vallero, 2010;HuiJie et al, 2011;Mao et al, 2012;Festa et al, 2013;Gallego et al, 2013;Nzila, 2013;Cui et al, 2014). Bacterial consortia have a synergistic effect because complementary PAH degradation pathways promote crossfeeding and avoid the buildup of inhibiting metabolites (Bouchez et al, 1999).…”

Section: Pah-degrading Marine Bacteriamentioning

confidence: 99%

Polycyclic aromatic hydrocarbons in deep sea sediments: Microbe–pollutant interactions in a remote environment

Louvado

Gomes

Simões

et al. 2015

Science of The Total Environment

104

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“…; Festa et al . ). Sphingomonas species are characterized by their large catabolic diversity, being able to degrade a wide range of natural and anthropogenic aromatic compounds (Stolz ).…”

Section: Introductionmentioning

confidence: 97%

“…Sphingomonas ('sensu latu') present four subgenera proposed by Takeuchi et al (2001) called Sphingobium, Novosphingobium, Sphingopyxis and Sphingomonas ('sensu stricto'). This genus comprises PAH-degrading microorganisms present in soil, which are frequently isolated from PAH-enriched cultures (Baraniecki et al 2002;Festa et al 2013). Sphingomonas species are characterized by their large catabolic diversity, being able to degrade a wide range of natural and anthropogenic aromatic compounds (Stolz 2009).…”

Section: Introductionmentioning

confidence: 99%

Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strainSphingobiumsp. 22B

et al. 2018

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Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil

Cited by 46 publications

References 46 publications

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Polycyclic aromatic hydrocarbons in deep sea sediments: Microbe–pollutant interactions in a remote environment

Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strainSphingobiumsp. 22B

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