Bacterial Diversity in a Sri Lankan Geothermal Spring Assessed by Culture-Dependent and Culture-Independent Approaches

Samarasinghe, Supun N; Wanigatunge, R.P.; Magana-Arachchi, Dhammika

doi:10.1007/s00284-021-02608-4

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…The work also reported the abundance of thermophilic actinobacteria which holds industrial importance as a source of several enzymes such as pullulanase, amylase, DNA polymerase 33 . Proteobacteria and Firmicutes were reported in major proportion of the microbial population in Sri-Lankan Geothermal spring by Samarasinghe et al 34 . They reported the presence of microbiota in surface water and in-depth water samples, which is lacking in the present report.…”

Section: Resultsmentioning

confidence: 95%

Exploring microbial diversity in hot springs of Surajkund, India through 16S rRNA analysis and thermozyme characterization from endogenous isolates

Soy,

Lakra,

Prakash

et al. 2023

Sci Rep

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Hot springs are a valuable source of biologically significant chemicals due to their high microbial diversity. To investigate the possibilities for industrial uses of these bacteria, researchers collected water and sediment samples from variety of hot springs. Our investigation employed both culture-dependent and culture-independent techniques, including 16S-based marker gene analysis of the microbiota from the hot springs of Surajkund, Jharkhand. In addition, we cultivated thermophilic isolates and screened for their ability to produce amylase, xylanase, and cellulase. After the optimized production of amylase the enzyme was partially purified and characterized using UPLC, DLS-ZP, and TGA. The retention time for the amylase was observed to be around 0.5 min. We confirmed the stability of the amylase at higher temperatures through observation of a steady thermo gravimetric profile at 400 °C. One of the thermophilic isolates obtained from the kund, demonstrated the potential to degrade lignocellulosic agricultural waste.

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Section: Resultsmentioning

confidence: 95%

Exploring microbial diversity in hot springs of Surajkund, India through 16S rRNA analysis and thermozyme characterization from endogenous isolates

Soy,

Lakra,

Prakash

et al. 2023

Sci Rep

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“…The present work also reports the presence of industrially important thermostable enzymes producers such as xylanases, amylases and cellulases. Proteobacteria and Firmicutes were reported in a major proportion of the microbial population by Samarasinghe et al (2021) [31]. They reported the metagenomic analysis of Sri-Lankan Geothermal spring with culture dependent and independent approaches.…”

Section: Metagenomic Sequencing Of the Isolated Dnamentioning

confidence: 99%

“…and Pannonibacter sp. were found to be common [31]. Anoxygenic photosynthesis performing bacteria were reported to be found in the Rhodobacteraceae family.…”

Section: Metagenomic Sequencing Of the Isolated Dnamentioning

confidence: 99%

Metagenomic analysis of Hot Springs of Surajkund and the exploration of thermostable enzymes from endogenous isolates

Soy

Lakra

Prakash

et al. 2023

Preprint

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Hot springs serve as a hub for microbial diversity, the microflora of the hot springs can be a source of numerous biologically important molecules. Extremophile bacteria were isolated from different hot springs of India to investigate their potential in industrial applications. The analysis involved both culture dependent and culture independent methods. The metagenomic analysis of the microflora was carried out using next-gen sequencing. Also, thermophilic isolates were culture and primarily screened for amylase, xylanase and cellulase enzyme production. The production for amylase was optimized and partial purification was carried out. Partially purified amylase was tested and characterized using; UPLC, DLS-ZP and TGA. The retention time for the amylase was observed to be around 0.5 minutes along with a stable thermo gravimetric profile around 400oC confirming its stability at higher temperatures. One of the thermophilic isolate was able to degrade lignocellulosic waste exhibiting the potential of isolates from the kund.

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“…Thermophilic bacteria are highly valued for their species diversity, molecular phylogeny, and generation of secondary metabolites due to their extreme habitat (Fandi et al, 2014;Narsing Rao et al, 2021). Two broad categories of methods are utilized to study bacterial diversity in hot springs: culture-independent and culture-dependent techniques, with the latter being traditionally used for isolating isolates and investigating their properties (Simoes et al, 2007;Samarasinghe et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

Al-Harthy,

Hozzein,

Abdelkader

et al. 2024

Egypt. J. Microbiol.

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H OT springs are known to be rich in diverse microorganisms, including thermophilic bacteria capable of producing industrially important enzymes. This study aimed to isolate, purify, and identify thermophilic bacteria from three hot springs located in the southern region of Saudi Arabia, namely Al-Ma'a Al-Har, Al-Aredhah, and Al-Ahsarai hot spring. The isolated bacterial isolates were screened for amylase enzyme production, and the most potent isolate, Cytobacillus firmus OQ834432 isolate OHA8, was selected for large-scale production of the amylase enzyme. The enzyme was then purified using salt precipitation, Sephacryl S-200 chromatography, and DEAE-Sepharose column chromatography. A total of twenty-one bacterial species were identified, with Cytobacillus firmus exhibiting the highest amylase activity. The optimal conditions for the amylase enzyme activity were found to be at 50°C and pH 5.0, with a 153% enhancement in activity in the presence of magnesium ions. The enzyme's activity was inhibited by EDTA and was found to be active with starch and amylopectin at 100% and 110.3%, respectively. In conclusion, this study demonstrates the potential of thermophilic bacteria from hot springs to produce industrially important enzymes and provides insights into the optimal conditions for enzyme production and activity. These findings have significant implications for the development of biotechnological applications utilizing thermophilic bacteria in various industrial sectors.

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Bacterial Diversity in a Sri Lankan Geothermal Spring Assessed by Culture-Dependent and Culture-Independent Approaches

Cited by 10 publications

References 42 publications

Exploring microbial diversity in hot springs of Surajkund, India through 16S rRNA analysis and thermozyme characterization from endogenous isolates

Exploring microbial diversity in hot springs of Surajkund, India through 16S rRNA analysis and thermozyme characterization from endogenous isolates

Metagenomic analysis of Hot Springs of Surajkund and the exploration of thermostable enzymes from endogenous isolates

Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

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