Bacterial Endophyte Inhabiting Durio zibethinus and its Radical Scavenging and Antidiabetic Potential

Abstract

Background: Endophytes, markedly receiving interest and has been detected to be remarkable sources of bioactive metabolites. Endophytes, which are also called as endosymbionts have pulled into consideration in the discovery for novel bioactive compounds that can be utilized as new medications. In the current study, endophyte has been isolated from Durio zibethinus leaves. 16s rRNA partial genome sequencing was used to identify selected endophyte and the obtained bacterial endophytic crude extract (DZLM) was subjected for the antioxidant and antidiabetic investigations. Results: Cronobacter sakazakii was identifies as the endophye. Qualitative phytochemical test on DZLM confirmed the presence of phenols, carbohydrates, alkaloids, flavonoids, steroids, mucilage, and glycosides. The antioxidant assay of DZLM showed the good antioxidant potency with IC 50 ±SEM: 161±0.08 µg/mL for DPPH assay and IC 50 ±SEM: 126± 0.09 µg/mL for ABTS assay. Antidiabetic assay results demonstrated a dosedependent percentage inhibition of the enzymes. The results revealed good inhibition of α-amylase with IC 50 ±SEM: 239±0.08µg/mL and α-glucosidase inhibition with IC 50 ±SEM: 241±0.08 µg/mL for DZLM compared to the standard drug, acarbose. Conclusion: The study uncovered the fact that DZLM has a wellspring of natural source of antioxidant and antidiabetic agents and further studies are required for fractionation of the active constituents existing in DZLM and also their structural elucidation which supports the application of endo-symbionts as the basis of future natural medicines.

Nutritional, pharmaceutical, and functional aspects of rambutan in industrial perspective: An updated review

“…Chigurupati et al (2019) worked on the nutritional composition of rambutan and reported the presence of total flavonoid contents and total phenolic content in terms of gallic acid and rutin equivalents; however, antioxidant function of rambutan inhibited α‐amylase, α‐glucosidase enzyme activities and inhibition of ABTS radicals along with DPPH activity. The biochemical composition of fruit is indicated in Table 1.…”

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

“…Therefore, developing safe and natural new drugs for this disease is necessary. Chigurupati et al (2021) recorded the incredible capacity of endophytic microbes to produce bioactive compounds as an antioxidant and antidiabetic agents from Durio zibethinus. Moreover, the endophytic bacteria studies on pteridophyte (ferns and its allies) species has been reported on many taxa, e.g., Dryopteris (Das et al 2017), Ophioglossum (Mukherjee et al 2017), Azolla (Yang et al 2022), and Asplenium (Masocha et al 2022).…”