Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities

Abstract

The aim of this study was to screen bacterial endophytes with antibacterial and enzyme activity from mangrove leaves of Indonesia. Methodology and results: Bacterial endophytes were isolated and evaluated for antibacterial activity against five strains of pathogenic bacteria. Enzymatic Index (EI) was measured to evaluate the production of protease, amylase and cellulase. Hemolysin test was performed on Blood Agar and the sensitivity to antibiotic was performed. Bacterial endophyte Strain 1-1 isolated from Bruguiera gymnorrhiza showed strong inhibition against Escherichia coli, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa with inhibition zone of 12.6 ± 1.4, 8.8 ± 4.1, 12.5 ± 2.3 and 8.4 ± 0.9 mm respectively. Isolate 1-16 which isolated from B. gymnorrhiza exhibited antibacterial activity against E. coli and P. aeruginosa, while Isolate 6-10 isolated from Avicennia lanata exhibited strong inhibition on Salmonella sp. (13.1 ± 3.3 mm). All of those three isolates produced protease, non-haemolysin-producing strain and sensitive to gentamicin or kanamycin but resistant to ampicillin, tetracycline and chloramphenicol. Those three isolates were identified based on homology of 16S rDNA sequence. Strain 1-1 and 1-16 were identified as P. aeruginosa, while strain 6-10 identified as S. marcescens. Conclusion, significance and impact of study: This finding was showed the potential endophytic bacteria from Indonesian mangrove plants with antibacterial and enzyme production.

Study on the community structure and function of symbiotic bacteria from different growth and developmental stages of Hypsizygus marmoreus

“…To further elucidate the colonization of the symbiotic species Serratia odorifera HZSO-1, PCR amplification, and fluorescence labeling were used. The results confirmed the bacterium to be a symbiotic species residing in the hyphae of H. marmoreus , providing a theoretical basis for clarifying the colonization of mushroom symbiotic bacteria and for developing mushroom growth-promoting agents made from S. odorifera that can be used in the production of edible fungi [ 32 , 33 ].…”

Study on the community structure and function of symbiotic bacteria from different growth and developmental stages of Hypsizygus marmoreus

“…To further elucidate the colonization of the symbiotic species Serratia odorifera HZSO-1, PCR ampli cation, and uorescence labeling were used. The results con rmed the bacterium to be a symbiotic species residing in the hyphae of H. marmoreus, providing a theoretical basis for clarifying the colonization of mushroom symbiotic bacteria and for developing mushroom growth-promoting agents made from S. odorifera that can be used in the production of edible fungi [32,33].…”

Isolation and Screening of Proteolytic Bacteria from Rice-Fish Farming System Sediments

“…Bacteria isolates with proteolytic activity showed a clear zone around the colony. The diameter of both bacteria colony and the clear zone formed was measured to determine proteolytic activity index [30,31]. The measurement results were entered into proteolytic activity calculation formula as quantitative data [26].…”