Bacterial lipid biophysics and membrane organization

Mitchison-Field, Lorna My; Belin, Brittany J

doi:10.1016/j.mib.2023.102315

Cited by 20 publications

(5 citation statements)

References 174 publications

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“…Alterations in phospholipid constituents may induce impaired membrane functionality. 51,52 Perturbation of the glycerophospholipid metabolic pathway may be an important mechanism by which EVs disrupted the structure and function of the S. aureus cell membrane. Arginine is a fundamental amino acid in protein synthesis and plays a pivotal role in cell growth, physiological activity, and stress response.…”

Section: ■ Discussionmentioning

confidence: 99%

“…This, in turn, has an impact on the composition of the lipid spatial domains and the recognition capacity of membrane proteins. Alterations in phospholipid constituents may induce impaired membrane functionality. , Perturbation of the glycerophospholipid metabolic pathway may be an important mechanism by which EVs disrupted the structure and function of the S. aureus cell membrane.…”

Section: Discussionmentioning

confidence: 99%

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Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Liu,

Guo,

et al. 2024

ACS Infect. Dis.

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The colonizing microbiota on the body surface play a crucial role in barrier function. Staphylococcus aureus (S. aureus) is a significant contributor to skin infection, and the utilization of colonization resistance of skin commensal microorganisms to counteract the invasion of pathogens is a viable approach. However, most studies on colonization resistance have focused on skin bacteria, with limited research on the resistance of skin fungal communities to pathogenic bacteria. Extracellular vehicles (EVs) play an important role in the colonization of microbial niches and the interaction between distinct strains. This paper explores the impact of Malassezia restricta (M. restricta), the fungus that dominates the normal healthy skin microbiota, on the proliferation of S. aureus by examining the distribution disparities between the two microorganisms. Based on the extraction of EVs, the bacterial growth curve, and biofilm formation, it was determined that the EVs of M. restricta effectively suppressed the growth and biofilm formation of S. aureus. The presence of diverse metabolites was identified as the primary factor responsible for the growth inhibition of S. aureus, specifically in relation to glycerol phospholipid metabolism, ABC transport, and arginine synthesis. These findings offer valuable experimental evidence for understanding microbial symbiosis and interactions within healthy skin.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Liu,

Guo,

et al. 2024

ACS Infect. Dis.

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“…Though it has not been tested directly, it is possible that the levels of some or all hopanoids are also linked to GSR activation in B. diazoefficiens , or to the activation of membrane-specific stress response pathways. Another hypothesis is that hopanoids affect membrane-based processes through their role in biophysically reinforcing or compartmentalizing cell membranes, reviewed in ( 36 ). Bacterial flagellar motility, chemotaxis, protein secretion, and the nitrate assimilatory pathway all involve large membrane-associated protein complexes that may rely on proper membrane mechanics to function.…”

Section: Discussionmentioning

confidence: 99%

Hopanoid lipids promote soybean –Bradyrhizobium symbiosis

Pan,

Shim,

Lubin

et al. 2024

mBio

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The symbioses between leguminous plants and nitrogen-fixing bacteria known as rhizobia are well known for promoting plant growth and sustainably increasing soil nitrogen. Recent evidence indicates that hopanoids, a family of steroid-like lipids, promote Bradyrhizobium symbioses with tropical legumes. To characterize hopanoids in Bradyrhizobium symbiosis with soybean, we validated a recently published cumate-inducible hopanoid mutant of Bradyrhizobium diazoefficiens USDA110, Pcu- shc ::∆ shc . GC-MS analysis showed that this strain does not produce hopanoids without cumate induction, and under this condition, is impaired in growth in rich medium and under osmotic, temperature, and pH stress. In planta , Pcu- shc ::∆ shc is an inefficient soybean symbiont with significantly lower rates of nitrogen fixation and low survival within the host tissue. RNA-seq revealed that hopanoid loss reduces the expression of flagellar motility and chemotaxis-related genes, further confirmed by swim plate assays, and enhances the expression of genes related to nitrogen metabolism and protein secretion. These results suggest that hopanoids provide a significant fitness advantage to B. diazoefficiens in legume hosts and provide a foundation for future mechanistic studies of hopanoid function in protein secretion and motility. IMPORTANCE A major problem for global sustainability is feeding our exponentially growing human population while available arable land decreases. Harnessing the power of plant-beneficial microbes is a potential solution, including increasing our reliance on the symbioses of leguminous plants and nitrogen-fixing rhizobia. This study examines the role of hopanoid lipids in the symbiosis between Bradyrhizobium diazoefficiens USDA110, an important commercial inoculant strain, and its economically significant host soybean. Our research extends our knowledge of the functions of bacterial lipids in symbiosis to an agricultural context, which may one day help improve the practical applications of plant-beneficial microbes in agriculture.

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“…46 For example, the asymmetric distribution of zwitterionic, anionic and cationic headgroups between the inner and outer membrane leaflet are highly controlled for the optimum surface electrostatic charge to maintain proper protein topologies and assembly. 25,47,48 The charged head groups are also important in the selectivity of AMPs for targeting of membranes.…”

Section: Bacterial Membrane Lipid Profilesmentioning

confidence: 99%