Bacterial Panicle Blight Resistance QTLs in Rice and Their Association with Other Disease Resistance Loci and Heading Date

Abstract

Bacterial panicle blight (BPB) of rice (Oryza sativa L.) occurs when the bacterium Burkholderia glumae Kurita and Tabei infects emerging and flowering panicles, causing kernels to abort. To identify quantitative trait loci (QTLs) for BPB resistance, a population of 300 recombinant inbred lines (RILs) derived from a cross between ‘Lemont’ and ‘TeQing’ were evaluated in 2001 and 2002 in field plots spray‐inoculated with B. glumae at the time of flowering. Because this RIL population had been previously used to map QTLs for three other diseases, present use of this population allowed direct comparison between the various disease resistance QTLs. Multiple interval mapping using QTL Cartographer v2.5 putatively identified 12 BPB QTLs, three of which were statistically significant in both years and found to have epistatic effects in 2002. TeQing was the source of resistance for eight QTLs; Lemont for four. Four BPB QTLs colocated with QTLs previously identified as providing resistance to one or multiple other diseases. Three BPB QTLs were also associated with late flowering. Because late flowering panicles are subjected to cooler temperatures that are less conducive to disease development during grain fill, it is possible that the genetic effects of the heading‐related QTLs were biased. The present data could not distinguish between pleiotropy and close linkage of the BPB QTLs with the previously identified heading and disease resistance QTLs.

Mapping and Validation of Quantitative Trait Loci Associated with Tiller Production in Rice

“…Hoshikawa (1989) provides a lengthy description of rice plant growth, including tiller production. 1999TN 2002TN 2003TN 1998N1T 1999N1T 2002N1T 2003N1T 1998 (1998, 1999, 2002, and 2003) per panicle (Li et al, 1997); panicle weight (Li et al, 1998); tiller angle (Li et al, 1999b); disease resistance (Pinson et al, 2005(Pinson et al, , 2010 Mutations: OsmiR393, Xia et al, 2012QTLs: Wang et al, 1994Yan et al, 1998;nagata et al, 2002;Xing et al, 2002;Ramalingam et al, 2003;Miyamoto et al, 2004;Zhang et al, 2005; Blast resistance (Tabien et al, 2002); 100-kernel weight (Paterson et al, 1995;Li et al, 1997); grain no. Pearson correlations between four Lemont ´ TeQing recombinant inbred line (LT-RIL) observation trials (years) and between traits potentially associated with the primary trait of interest, the max.…”

“…number of tillers produced per potted 9-wk seedling. per panicle (Li et al, 1997); disease resistance (Li et al, 1999a;Pinson et al, 2005Pinson et al, , 2010 Mutations: tillering and dwarf 1 (tad1), Xu et al (2012). tiller number per plant (TN), node of first tiller (N1T), average number of seed-bearing panicles per plant (observed only from the 1998 trial), and average number of leaves (nodes) produced by 8-wk-old seedlings (SLN).…”

Identification of qRBS1, a QTL involved in resistance to bacterial seedling rot in rice

“…We tested Nona Bokra and found that it was not resistant to grain rot (data not shown). In addition, the chromosomal position of qRBS1 is different from those of QTLs reported to be associated with resistance to grain rot (Mizobuchi et al 2013; Pinson et al 2010). Therefore, the factors associated with resistance to seedling rot and grain rot appear to be different.…”

“…Many studies have been performed to understand the genetic control of resistance to bacterial grain rot and several cultivars appear to be resistant to bacterial grain rot (Goto and Watanabe 1975; Groth et al 2007; Imbe et al 1986; Mogi and Tsushima 1985; Nandakumar et al 2007a; Nandakumar and Rush 2008; Pinson et al 2010; Prabhu and Bedendo 1988; Sayler et al 2006; Sha et al 2006; Takita et al 1988; Wasano and Okuda 1994; Yasunaga et al 2002). Using resistant cultivars, QTLs for resistance to bacterial grain rot have been reported (Mizobuchi et al 2013; Pinson et al 2010).…”

“…Using resistant cultivars, QTLs for resistance to bacterial grain rot have been reported (Mizobuchi et al 2013; Pinson et al 2010). In contrast, few reports about resistance to BSR have been published, because BSR resistance is a complex characteristic influenced by environmental factors (Azegami 2009; Goto 1982; Mogi 1984a, b, c).…”