Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Weckwerth, Paulo Henrique; Franco, Adriana Terezinha de Mattias; Lopes, Carlos Alberto de Magalhães; Santos, Fernando dos; Weckwerth, Ana Carolina Villas Bôas; Vivan, Rodrigo Ricci; Duarte, Marco Antônio Húngaro

doi:10.1597/11-325

Cited by 7 publications

(8 citation statements)

References 47 publications

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“…reported that Pseudomonas aeruginosa was somewhat prevalent in otitis media secretions, while other species, such as S. aureus , E. faecalis , and Proteus mirabilis , occurred at a lower level of frequency ( Weckwerth et al., 2009 ). A later study by the same group found P. aeruginosa to be the most common species and moderately prevalent, and S. aureus to also be prevalent ( Weckwerth et al., 2014 ). Interestingly, when PCR analysis was utilized, the most prevalent species appeared to be F. nucleatum , in nearly 40% of samples ( Weckwerth et al., 2014 ).…”

Section: Resultsmentioning

confidence: 99%

“…A later study by the same group found P. aeruginosa to be the most common species and moderately prevalent, and S. aureus to also be prevalent ( Weckwerth et al., 2014 ). Interestingly, when PCR analysis was utilized, the most prevalent species appeared to be F. nucleatum , in nearly 40% of samples ( Weckwerth et al., 2014 ). These studies are in contrast to an earlier publication by Jousimies-Somer and Rintala, in which M .…”

Section: Resultsmentioning

confidence: 99%

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Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Gershater

Liu

Xue

et al. 2023

Front. Cell. Infect. Microbiol.

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Orofacial cleft disorders, including cleft lip and/or palate (CL/P), are one of the most frequently-occurring congenital disorders worldwide. The health issues of patients with CL/P encompass far more than just their anatomic anomaly, as patients with CL/P are prone to having a high incidence of infectious diseases. While it has been previously established that the oral microbiome of patients with CL/P differs from that of unaffected patients, the exact nature of this variance, including the relevant bacterial species, has not been fully elucidated; likewise, examination of anatomic locations besides the cleft site has been neglected. Here, we intended to provide a comprehensive review to highlight the significant microbiota differences between CL/P patients and healthy subjects in various anatomic locations, including the teeth inside and adjacent to the cleft, oral cavity, nasal cavity, pharynx, and ear, as well as bodily fluids, secretions, and excretions. A number of bacterial and fungal species that have been proven to be pathogenic were found to be prevalently and/or specifically detected in CL/P patients, which can benefit the development of CL/P-specific microbiota management strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Gershater

Liu

Xue

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Weckwerth et al [ 41 ] conducted a study with 31 patients with CLP and chronic suppurative otitis media and obtained positive cultures from 83% of the patients. Pseudomonas aeruginosa (54.9%) , Staphylococcus aureus (25.9%) and Enterococcus faecalis (19.2%) were isolated, but no anaerobes were isolated by culture, and the polymerase chain reaction assays revealed 1 or more bacteria in 97.1% of the samples.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the oral microbiota and the relationship of the oral microbiota with the dental and periodontal status in children and adolescents with nonsyndromic cleft lip and palate. Systematic literature review and meta-analysis

Escobar-Arregocés,

Eras,

Bustos

et al. 2024

Clin Oral Invest

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Objective To identify the characteristics of the oral microbiota and the relationship of the dental caries and periodontal status in patients aged 0 to 18 years with non-syndromic cleft lip and palate (CLP). Materials and methods A systematic review of the literature was carried out. Five databases were consulted, including publications in English, Spanish and Portuguese. The evaluations of the quality of the observational studies and the experimental studies were carried out with the Newcastle–Ottawa scale and CONSORT guidelines, respectively. The risk of bias of the studies was determined using Rev Manager 5.4, and 5 publications were meta-analyzed. Results The cariogenic microbiota of children and adolescents with cleft lip and palate was similar to that of children without clefts, although with higher counts of Streptococcus mutans and Lactobacillus spp. The periodontopathogenic microbiota was related to the presence of Campylobacter spp, Fusobacterium spp, Fusobacterium nucleatum, Prevotella intermedia/nigrescens, Parvimonas micra and Porphyromonas gingivalis, considered microorganisms with high pathogenic capacity. Heterogeneity was shown in relation to the microbiota and the type of fissure, presenting numerous microorganisms associated with the pre- and post-surgical condition (cheilorrhaphy and palatorrhaphy) such as Staphylococcus aureus, Streptococcus beta hemolyticus, Klebsiella pneumoniae and Klebsiella oxytoca, Moraxella catarrhalis, Candida spp, Candida albicans, Candida krusei and Candida tropicalis. The meta-analysis revealed that patients with cleft lip and palate were 2.03 times more likely to have caries than the control group (p<0.005). Conclusion In the microbiota, there was a great diversity of microorganisms that can vary according to the type of fissure and surgical interventions predisposing patients to a greater probability of dental caries, it is important to take into account the technique used to describe the oral microbiota in order to be able to compare the different studies. Clinical relevance Studying the microbiota and the relationship of dental caries and periodontal status in children and adolescents with cleft lip and palate can facilitate the comprehensive care of patients with these conditions.

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“…Weckwerth et al [39] conducted a study with 31 patients with CLP and chronic suppurative otitis media and obtained positive cultures from 83% of the patients. Pseudomonas aeruginosa (54.9%), Staphylococcus aureus (25.9%) and Enterococcus faecalis (19.2%) were isolated, but no anaerobes were isolated by culture, and the polymerase chain reaction assays revealed 1 or more bacteria in 97.1% of the samples.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the oral microbiota and the relationship of the oral microbiota with the dental and periodontal status in children and adolescents with nonsyndromic cleft lip and palate. Systematic literature review and meta-analysis

Castellano

Iñahuazo

Pardo

et al. 2023

Preprint

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Objective To identify the characteristics of the oral microbiota and the relationship of the dental caries and periodontal status in patients aged 0 to 18 years with nonsyndromic cleft lip and palate (CLP).Material and methods A systematic review of the literature was carried out. Five databases were consulted, including publications in English, Spanish and Portuguese. The evaluations of the quality of the observational studies and the experimental studies were carried out with the Newcastle–Ottawa scale and CONSORT guidelines, respectively. The risk of bias of the studies was determined using Rev Manager 5.4, and 5 publications were meta-analyzed.Results In cariogenic microbiota, Lactobacillus spp. and Streptococcus mutans were identified at high levels, and the related periodontopathogenic microorganisms were Prevotella intermedia, Prevotella nigrescens, Porphyromonas gingivalis, Fusobacterium spp., Campylobacter spp., and Fusobacterium nigrescens. The microorganisms found in pre- and postoperative interventions were Staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Streptococcus pyogenes, Moraxella catarrhalis, methicillin-resistant Staphylococcus aureus and Candida spp., with great heterogeneity in microbiological counts. The meta-analysis revealed that patients with CLP were 2.03 times more likely to have cavities than was the control group (p < 0.005).Conclusion In the microbiota, there was a great diversity of microorganisms that can vary according to the type of fissure and surgical interventions predisposing patients to a greater probability of dental caries.Clinical relevance To study the oral microbiota in CLP that facilitates the progression of dental caries and periodontal disease, take it into account in clinical decision-making and adopt preventive measures to reduce oral comorbidities.

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Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Abstract: Patients with cleft lip and palate with chronic suppurative otitis media presented high frequency of bacterial infection in the middle ear. The classical bacteriological culture did not detect strict anaerobes, whose presence was identified by the polymerase chain reaction method.

Cited by 7 publications

References 47 publications

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Characterization of the oral microbiota and the relationship of the oral microbiota with the dental and periodontal status in children and adolescents with nonsyndromic cleft lip and palate. Systematic literature review and meta-analysis

Characterization of the oral microbiota and the relationship of the oral microbiota with the dental and periodontal status in children and adolescents with nonsyndromic cleft lip and palate. Systematic literature review and meta-analysis

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