Bacterial RNA Chaperones Confer Abiotic Stress Tolerance in Plants and Improved Grain Yield in Maize under Water-Limited Conditions

Castiglioni, Paolo; Warner, Dave; Bensen, Robert J.; Anstrom, Don C.; Harrison, John A.; Stoecker, Martin; Abad, Mark S.; Kumar, Ganesh; Salvador, Sara; D’Ordine, Robert L.; Vilanova, Santiago; Back, Stephanie L.; Fernandes, Mary; Targolli, Jayaprakash; Dasgupta, Santanu; Bonin, Christopher; Luethy, Michael H.; Heard, Jacqueline E.

doi:10.1104/pp.108.118828

Cited by 432 publications

(279 citation statements)

References 37 publications

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“…Nevertheless, transgenic maize plants overexpressing ZmNF-YB2 were darker green and flowered 1 to 3 d earlier than the control plants under conditions of ample water supply (Nelson et al, 2007). Additionally, enhanced vegetative tolerance and improved kernel yield under water deficit conditions were produced by the overexpression of ZmNF-YB2, betA, or Csp genes in maize (Quan et al, 2004;Nelson et al, 2007;Castiglioni et al, 2008). Continued evaluation of ZmASR1-OE across different hybrid genetic backgrounds adapted for the marketplace will be important ongoing work.…”

Section: Zmasr1 Maintained Kernel Yield Under Water Deficit Conditionmentioning

confidence: 99%

“…Expression of a maize CAAT box transcription factor, Z. mays nuclear factor Y B subunit 2 (ZmNF-YB2), has also been shown to confer drought tolerance and enhanced photosynthetic capacity under drought stress, with improvements in kernel yield in maize (Nelson et al, 2007). Additionally, two members of a family of bacterial RNA chaperones, cold shock protein A (CspA) from E. coli and CspB from Bacillus subtilis, were shown to confer vegetative tolerance and improved end-of-season kernel yield under waterlimiting conditions in maize (Castiglioni et al, 2008). These studies clearly demonstrate that maize plants are amenable to improved water deficit tolerance through multiple mechanisms of action.…”

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The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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Abscisic acid-, stress-, and ripening-induced (ASR) proteins were first described about 15 years ago as accumulating to high levels during plant developmental processes and in response to diverse stresses. Currently, the effects of ASRs on water deficit tolerance and the ways in which their physiological and biochemical functions lead to this stress tolerance remain poorly understood. Here, we characterized the ASR gene family from maize (Zea mays), which contains nine paralogous genes, and showed that maize ASR1 (ZmASR1) was encoded by one of the most highly expressed paralogs. Ectopic expression of ZmASR1 had a large overall impact on maize yield that was maintained under water-limited stress conditions in the field. Comparative transcriptomic and proteomic analyses of wild-type and ZmASR1-overexpressing leaves led to the identification of three transcripts and 16 proteins up-or down-regulated by ZmASR1. The majority of them were involved in primary and/or cellular metabolic processes, including branched-chain amino acid (BCAA) biosynthesis. Metabolomic and transcript analyses further indicated that ZmASR1-overexpressing plants showed a decrease in BCAA compounds and changes in BCAA-related gene expression in comparison with wild-type plants. Interestingly, within-group correlation matrix analysis revealed a close link between 13 decreased metabolites in ZmASR1-overexpressing leaves, including two BCAAs. Among these 13 metabolites, six were previously shown to be negatively correlated to biomass, suggesting that ZmASR1-dependent regulation of these 13 metabolites might contribute to regulate leaf growth, resulting in improvement in kernel yield.

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Section: Zmasr1 Maintained Kernel Yield Under Water Deficit Conditionmentioning

confidence: 99%

mentioning

confidence: 99%

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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“…The first drought-tolerant crop was released in 2013, in which a bacterial gene encoding chaperonin was introduced into maize inbred lines and conferred drought resistance. 11 The estimated hectares of DroughtGard TM maize planted for the first time in the US in 2013 was 50,000 hectares and increased over 5 fold to 275,000 hectares in 2014 reflecting high acceptance by US farmers. 8 In addition, drought-tolerant sugarcane was developed by Ajinomoto Company by using the betA gene from Rhizobium meliloti.…”

Section: Food Security and Ge Cropsmentioning

confidence: 99%

Excavating abiotic stress-related gene resources of terrestrial macroscopic cyanobacteria for crop genetic engineering: dawn and challenge

Gao

2015

Bioengineered

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“…Fueron seleccionados tres genes denominados TaDREB3 AP2 (Lopato et al, 2006;Morran et al, 2011), AtZAT10 (Meissner y Michael,1997;Mittler et al, 2006) y CspB (Willimsky et al, 1992;Castiglioni et al, 2008), que pueden conferir tolerancia a sequía.…”

Section: Introductionunclassified

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

Carreño-Venegas

Chaparro-Giraldo

2016

Acta biol. Colomb.

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RESUMENComo primera aproximación en la obtención de una línea transgénica de maíz tolerante a sequía y al herbicida glufosinato de amonio, se seleccionaron genes y elementos reguladores para el diseño in silico de casetes de expresión, a través del análisis de literatura científica y bases de datos de genes y patentes. Las secuencias génicas fueron modificadas con base en el criterio de uso codónico del maíz para optimizar su expresión. Los casetes de expresión diseñados con el software DNA 2.0., fueron sintetizados por una empresa especializada. La presencia del transgen y la expresión a nivel de mARN fue demostrada mediante PCR y RT-PCR en la planta modelo Nicotiana benthamiana transformada vía Agrobacterium tumefaciens. Un ensayo preliminar in vitro en condiciones simuladas de sequía en medio MS con PEG (PM 6000)10 % no demostró incremento notorio en la tolerancia de las plántulas transformantes, posiblemente debido a que el uso codónico del diseño no favorece la expresión génica en la planta modelo.Palabras clave: estrés abiótico, tolerancia a herbicida, transgénesis, uso codónico. ABSTRACTAs a first approach in obtaining a transgenic drought and glufosinate ammonium tolerant maize line, genes and regulatory elements for the in silico design of the expression cassettes were selected through analysis of scientific literature and databases of genes and patents. Gene sequences were modified based on the criterion of maize codon usage to optimize their expression. The constructs designed with DNA 2.0 Software, were synthesized by a specialized company. The presence of the transgene and the expression of mRNA was demonstrated by PCR and RT-PCR in the model plant Nicotiana benthamiana transformed via Agrobacterium tumefaciens. A preliminary experiment in vitro under simulated drought conditions in MS medium with 10 % PEG (PM 6000) showed no noticeable increase in drought tolerance of the transformants, possibly because the codon usage of the design does not promote gene expression in the model plant.

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Bacterial RNA Chaperones Confer Abiotic Stress Tolerance in Plants and Improved Grain Yield in Maize under Water-Limited Conditions

Cited by 432 publications

References 37 publications

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

Excavating abiotic stress-related gene resources of terrestrial macroscopic cyanobacteria for crop genetic engineering: dawn and challenge

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

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