Bacteriocin and its biomedical application with special reference to Lactobacillus

Miglani, Rashi; Parveen, Nagma; Kumar, Ankit; Dewali, Seeta; Rawat, Gaurav; Mishra, Rojita; Panda, Amrita Kumari; Bisht, Satpal Singh

doi:10.1016/b978-0-323-90113-0.00001-8

Cited by 2 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since biofilm is formed by probiotics, their beneficial antibacterial and anti-inflammatory properties play an important role in the host immune system [ 23 , 24 ]. Our results showed that P1 and P2 were very strong biofilm formers, and this important property makes them potentially useful probiotics for industrial use.…”

Section: Discussionmentioning

confidence: 99%

“…0.02 mL of the solutions was distributed for 48 hours on MRS agar media following incubation. Catalase testing, Gram staining, and biochemical tests such as growth at 15 and 45°C, acid and gas production from glucose, NH3 production from arginine, and sugar fermentation including arabinose, cellobiose, mannitol, mannose, melebiose, raffinose, ribose, salicin, rhamnose, and xylose were used to identify the strains [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem‐Resistant Pseudomonas aeruginosa Strains

Ghiaei,

Ghasemi,

Shokri

2023

BioMed Research International

View full text Add to dashboard Cite

Introduction. With the increase of hospital infections due to the indiscriminate use of antibiotics, multidrug resistance has increased, decreasing the effectiveness of antibiotics against these infections. For this reason, the identification of alternative agents such as probiotics has been considered. The aim of this study was to isolate and identify effective probiotics against carbapenem-resistant Pseudomonas aeruginosa strains. Material and Methods. During a period of eight months, isolates of P. aeruginosa were collected from patients in three hospitals in Isfahan. The presence of metallo-beta-lactamase enzymes was determined by the combination disc test (CDT). The inhibitory and antimicrobial activities of 20 probiotic bacteria isolated from local dairy products against these strains were investigated by agar dilution. Two probiotic strains that showed broader inhibition results were selected, and the values of the lowest inhibitory concentration (MIC) and the lowest lethal concentration (MBC) and their antibiofilm effect were determined using the microtiter plate method. The concentration of organic acids was done by HPLC. Findings. Of the 100 samples isolated and identified, 61 samples (61%) exhibited multiple drug resistance (MDR) and were selected for further investigation. Phenotypic diagnosis of the presence of metallo-beta-lactamase enzymes revealed that 74.5% of the strains were positive. The results showed that these two probiotics killed P. aeruginosa strains after only one hour, and the inhibition mechanism was due to the presence of lactic acid and acetic acid. The antibiofilm effect of these two probiotics was at concentrations of 1/2 and 1/4. Conclusion. The two Lactobacillus isolates had potential antimicrobial and antibiofilm properties against all carbapenem-resistant P. aeruginosa strains, even at thinner dilutions. Considering the broad activity of this strain, it can potentially be used for biocontrol of these strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%