Bacteriocin, Plasmid and Pectolytic Diversity in Pseudomonas cepacia of Clinical and Plant Origin

Gonzalez, Carlos F.; Vidaver, Anne K.

doi:10.1099/00221287-110-1-161

Cited by 76 publications

(42 citation statements)

References 26 publications

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“…Stained cells were viewed by phase-contrast microscopy. Phytopathogenicity was tested as described by Gonzalez & Vidaver (1979). In brief, strains were grown for 24 h at 28 mC.…”

Section: Methodsmentioning

confidence: 99%

Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov.

Coenye¹,

Falsen²,

Hoste³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

183

193

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A polyphasic taxonomic study was performed on a group of isolates tentatively identified as Burkholderia cepacia, Ralstonia pickettii or Ralstonia paucula (formerly known as CDC group IVc-2). The isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. SDS-PAGE of whole-cell proteins and AFLP fingerprinting distinguished at least five different species, and this was confirmed by DNA-DNA hybridizations. 16S rDNA sequence analysis of representative strains indicated that these organisms belong to the β-subclass of the Proteobacteria, with the genera INTRODUCTIONThe majority of life-threatening bacterial lung infections in cystic fibrosis (CF) patients are caused by a limited spectrum of bacterial pathogens which include Pseudomonas aeruginosa, Staphylococcus aureus and Burkholderia cepacia (Gilligan, 1991 ;Govan & Deretic, 1996). Other organisms which can be found regularly in respiratory tract specimens of CF patients include Haemophilus influenzae, Stenotro- phomonas maltophilia, certain fungi (e.g. Aspergillus spp.) and viruses (e.g. respiratory syncytial virus) (Gilligan, 1991). In the absence of epidemic spread, B. cepacia only infects a small proportion of CF patients, but it has a major impact on both morbidity and mortality of infected patients and the medical and psychosocial implications of B. cepacia colonization and segregation of colonized and non-colonized patients as a means of infection control are enormous (LiPuma, 1998). Accurate identification of B. cepacia is crucial in patient management and infection control practices, but may be problematic, and misidentification is relatively common (LiPuma, 1998 Vandamme et al., 1999). This report describes the polyphasic taxonomic study which was used for the characterization of these and similar isolates from the environment and human clinical specimens. The genotypic and phenotypic characteristics warranted the classification of the isolates in a novel genus, for which we propose the name Pandoraea. The genus Pandoraea contains five species : Pandoraea apista sp. nov. (the type species), Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis (Wittke et al. 1997) comb. nov. (basonym Burkholderia norimbergensis). The name Burkholderia norimbergensis was proposed by Wittke et al. (1997) for a single bacterial isolate capable of hetrotrophic sulfur oxidation, which was isolated from an oxic water layer above a sulfide-containing sediment. METHODSBacterial strains and growth conditions. Strains used in this study are listed in Table 1. All strains were grown aerobically on Trypticase Soy Agar (BBL) at 37 mC unless otherwise indicated.Phenotypic characterization. Classical phenotypic tests were performed as described previously (Vandamme et al., 1993). API ZYM and API20 NE tests were performed according to the recommendations of the manufacturer (bioMe! rieux). Flagella staining was performed using a solution of tannic acid (2 g)\phenol (5 %, w\v)\KAl(SO % ) # (satura...

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“…Stained cells were viewed by phase-contrast microscopy. Phytopathogenicity was tested as described by Gonzalez & Vidaver (1979). In brief, strains were grown for 24 h at 28 mC.…”

Section: Methodsmentioning

confidence: 99%

Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov.

Coenye¹,

Falsen²,

Hoste³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

183

193

View full text Add to dashboard Cite

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“…Growth on Trypticase Soy Agar (Becton Dickinson) at 35 and 42 mC was observed for appearance and pigment. Phytopathogenicity was tested as described by Gonzalez & Vidaver (1979). In brief, strains were grown for 24 h at 28 mC.…”

Section: Methodsmentioning

confidence: 99%

Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients.

Coenye

LiPuma

Henry

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

126

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A polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (CF) patients in the USA. These strains were tentatively identified as Burkholderia cepacia, Burkholderia vietnamiensis and Burkholderia or Ralstonia sp. using biochemical tests and 16S rDNA-based PCR assays. Visual comparison of protein profiles indicated that they belonged to a single new group (' group 13 '). The polyphasic taxonomic data showed that 18 of these strains represent a new member of the B. cepacia complex, referred to in this report as B. cepacia genomovar VI, whereas the other five strains belonged to Burkholderia multivorans.

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“…The following conventional tests were performed with the TVV strains: tests for pigment production on nutrient agar and King B medium (359, growth at temperatures between 20 and 41°C on nutrient agar, growth on PCAT medium (12), oxidase activity (49), and onion pathogenicity (26). The API 20NE microtube system method (bioMkrieux, La Balme-les-Grottes, France) was used as a standardized method to determine nitrate reduction, hydrolysis of gelatin and esculin, glucose fermentation, arginine dihydrolase activity, production of indole, p-galactosidase activity, and urease activity.…”

Section: Methodsmentioning

confidence: 99%

Polyphasic Taxonomy in the Genus Burkholderia Leading to an Emended Description of the Genus and Proposition of Burkholderia vietnamiensis sp. nov. for N2-Fixing Isolates from Rice in Vietnam

Gillis

Van

Bardin

et al. 1995

International Journal of Systematic Bacteriology

388

212

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F-54501 Vandewre-les-Nancy, and URA CNRS 1977, Ecologie Microbienne, USBSE,The taxonomic position of nitrogen-fixing strains that were isolated from rhizosphere macerates of rice cultivated in the Binh Thanh region of Vietnam was determined by using polyphasic taxonomy. We determined the phylogenetic relationships of these organisms by performing DNA-rRNA hybridization experiments with a labeled rRNA probe from the type strain of Burkholderia cepacia, and we found that they belong to a single rRNA complex. Other members of this rRNA complex were also studied, and the N,-fixing strains were found to be closely related to B. cepacia. In addition, all members of the rRNA complex containing B. cepacia were studied by performing auxanographic and DNA-DNA hybridization experiments. Phenotypically and genotypically, the N,-fixing isolates constitute a single cluster together with two strains of clinical origin. These organisms constitute a new Burkholderia species, for which the name Burkholderia vietnamiensis is proposed; the type strain of this species is TVV75 (= LMG 10929). All members of this species can fix nitrogen. On the basis of our polyphasic taxonomy results and previously published data we concluded that the genus Burkholderia should be restricted to the following species: B. cepacia (the type species), Burkholderia mallei, Burkholderia pseudomallei, B. vietnamiensis, Burkholderia gladioli, Burkholderia caryophylli, Burkholderia plantarii, Burkholderia glumue, Burkholderia vandii, BurkhoMeria cocovenenans comb. nov., and Burkholderia andropogonis comb. nov. On the basis of genotypic and phenotypic results [Alcaligenes] eutrophus, [BurkhoZderia] solanacearum, and[Burkholderia] pickettii belong to two other clusters whose internal structures must be studied further.On the basis of the results of extensive phenotypic studies and DNA-rRNA and DNA-DNA hybridization experiments performed by Palleroni, Stanier, and their collaborators, the genus Pseudomonas was divided into five groups (24, 43, 46, 56). Additional phylogenetic data have shown that these groups are only very remotely related and that each of them contains species belonging to other genera (21-23, 70, 71). In addition to the former pseudomonads that have been shown to belong to the genus Xanthomonas and to a smaller group related to Pseudomonas diminuta and Pseudomonas vesicularis (21-23,43) classified in the recently described genus Brevundirnonas (52), three large groups of pseudomonads can be considered. Pseudomonas rRNA group I (43) is part of rRNA superfamily I1 (18) or the gamma subclass of the Proteobacteria (S), where it constitutes a separate rRNA complex (18,21,22,68); this group represents the authentic pseudomonads which are grouped with Pseudomonas aeruginosa, the type species (43). Although the internal relationships within this group have not been determined yet, it is evident that the genus Pseudomonas must be limited to this group and that all other Pseudomonas species have been generically misnamed as determined by phylogenetic da...

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Bacteriocin, Plasmid and Pectolytic Diversity in Pseudomonas cepacia of Clinical and Plant Origin

Cited by 76 publications

References 26 publications

Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov.

Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov.

Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients.

Polyphasic Taxonomy in the Genus Burkholderia Leading to an Emended Description of the Genus and Proposition of Burkholderia vietnamiensis sp. nov. for N2-Fixing Isolates from Rice in Vietnam

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