Bacteriological and Histopathological Studies on Vibrio Species Isolated from Naturally Infected Freshwater Fish in Delta Region, Egypt

A, El-Sharaby S. M.; AbdEl-Gaber, Mostafa M.; Tarabees, Reda; Khalil, Riad H.; N, Ali M.; El-Ballal, Salah

doi:10.17582/journal.aavs/2018/6.1.17.26

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…A study conducted on V. parahaemolyticus showed that the bacteria reached a viable state when the concentration of NaCl was elevated up to a level of 30% [45]. The results matched with some authors [46]. In contrast, [47] reported lower results.…”

Section: Discussionsupporting

confidence: 77%

Assessment of the Bacteriological Quality of Some Fish Products

Elbarbary,

Abd El-aziz,

Maky

et al. 2024

Egyptian Journal of Veterinary Sciences

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F ISH products are considered a nutritionally significant component of the human diet because they contain macronutrients (proteins, fats, and ash) and micronutrients (vitamins and minerals). Accordingly, 200 different commercial fish product samples (25 each of canned solid tuna, canned chunks tuna, canned crumbled tuna, canned anchovy, canned mackerel, canned sardine, herring, and tilapia fillets) of different brands were collected from various local and high supermarkets in Aswan City during 2020-2021. The samples were subjected to microbial quality assessment, and PCR was carried out to detect some virulence genes of fish-borne pathogens to evaluate the control measures for food safety. The results presented that the highest bacterial loads (cfu/g) in the examined fish products were proven in fillets tilapia (1.3×104±0.32) and herring (1.0×104±1.2) while anchovy, mackerel, and crumbled tuna were the highest anaerobic count with means of 7.3×103±1.2, 7×103±1.6 and 6.7×103±1.4, respectively. The prevalence of P. aeruginosa occurs with an incidence of 29%. The lasB gene (50%) has been the most detected virulence gene, followed by exoS (40%). V. parahaemolyticus was present in 7.5%, and the regulator toxin genes (toxR and L.tdh) were found in 33.3% and 22.2%. Additionally, 20% of the samples had A. hydrophila, and the aerA and act genes were found in 60% of the isolates. In addition, C. perfringens was detected in 33% of examined products, and the virulence genes cpa and etx were present and discovered to be a proportion of 20% and 50%, respectively. The current study concluded that the examined fish products possess one or more food poisoning virulence genes indicating unsanitary handling, transport, processing, storage practices, and inappropriate environmental conditions.

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Section: Discussionsupporting

confidence: 77%

Assessment of the Bacteriological Quality of Some Fish Products

Elbarbary,

Abd El-aziz,

Maky

et al. 2024

Egyptian Journal of Veterinary Sciences

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“…Lamellar edemas (aneurysms) were also observed, albeit less frequently, in the gills of all the specimens exposed to Dormex ® (Figure 4D). The histopathological effects of pesticides on fish have been studied widely, and include alterations to the gills, liver, blood vessels, and kidney (Castro, Silva, Freitas, & Carvalho-Neta, 2014;El-Sharaby et al, 2018;Velmurugan, Cengiz, Yolcu, Uğurlu, & Selvanayagam, 2020). In fish, the gills initially respond through hyperplasia, which results in increased cell and tissue function, caused by physiological changes (Takashima & Hibiya, 1995).…”

Section: Histological Evaluationmentioning

confidence: 99%

Evidence of cytogenetic and histological damage in specimens of Astyanax lacustris (Pisces, Characidae) exposed to the hydrogen cyanide-based herbicide Dormex®

Zafra-Lemos

Lopes

Rampazzo

et al. 2021

Acta Sci. Biol. Sci.

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The herbicide Dormex®, a solution of hydrogen cyanamide, is a growth regulator capable of breaking the dormancy of fruit plants, and is commonly applied in agriculture. However, the biological effects of this product on non-target organisms are unknown. The present study investigated the biological response of Astyanax lacustris (Lütken, 1875) specimens exposed to Dormex® using a chromosome aberration test, the mitotic index, and the histological analysis of the gills. Forty specimens of Astyanax lacustris were obtained from a local breeding facility and divided into 10 groups (nine experimental and one control) with four fish in each aquarium (group). The control group was maintained for 24 hours in dechlorinated water while the experimental groups were allocated to one of nine different treatments, with three concentrations of Dormex®, 0.05, 0.1 and 0.5 mL L-1, and exposure for 24, 48 and 72 hours. The fish exposed to Dormex® presented chromosomal aberrations of a number of types, including chromosomal breaks, acentric fragments, decondensation, and gaps at the three Dormex® concentrations, at all exposure times. The mitotic index decreased significantly in comparison with the control group. The histological preparations of the gills revealed alterations such as hyperplasia, and lamellar fusion and edema, whereas in the control group the structure of the gills was preserved. The cytogenetic analysis revealed the genotoxic potential of the herbicide Dormex® and the morphological alterations of the gills demonstrated the sensitivity of the fish, which responded rapidly to the stressor. These findings reinforce the need for special care and restrictions on the use of these herbicides in agricultural areas located near aquatic environments.

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