Bacteriological study of West African clam (Egeria radiata Lamarch) and their overlying waters

Ekanem, E.O.; Adegoke, G. O.

doi:10.1016/s0740-0020(95)80119-7

Cited by 20 publications

(13 citation statements)

References 10 publications

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“…They concluded that the bacterial community of tropical brackish water shrimps cannot be predicted from the microbiota of their aquatic environment. The results of this work corroborate some reports on the bacteriology of seafood (43,44,45).…”

Section: Discussionsupporting

confidence: 91%

Comparative study of microbial, proximate and heavy metal compositions of some gastropods, bivalve and crustacean seafood

Oranusi¹,

Effiong²,

Duru³

2018

Af J Clin Exp Micro

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Background: Seafood are known to be nutritionally rich, however, water bodies from where these foods are harvested are often burdened with wastes from industries, household and run-offs. Thus seafood can harbor pathogenic microorganisms and heavy metals which can pose a serious health hazard to consumers. Materials and Methods: In this study, 5kg each of Littorina littorea, Achatina fulica,Tympanotonos fuscatus, Dorsanum miran, Egeria radiate and Penaeus notialis were investigated for microbial, proximate and heavy metal qualities using standard microbiological and chemical methods of analysis. Results: The gastropod mollusks Tympanotonos fuscatus, Littorina littorea and Achatina fulica had significantly (p≤0.05) higher microbial loads compared to other samples. All the samples had significantly (p≤0.05) higher coliform counts compared to standard specifications. The microbial isolates from the samples included species of Bacillus, Klebsiellae, Citrobacter, Providencia, Pseudomonas, Staphylococcus aureus, Escherichia coli and Aspergillus niger, Aspergillus flavus, yeasts, Alternaria, Absidia, Rhizopus, and Mucor. Antibiogram of the isolates revealed multiple resistances with 79.55% and 66.67% resistance to antibacterial and antifungal agents respectively. The seafood was rich in protein (8.0±0.03 to 46.0±0.03%) and fat (0.6±0.01 to 8.8±1.00%) but low in carbohydrate (1.18±0.84 to 3.81±0.86%).The heavy metal compositions of the seafood were generally low except for manganese 6.36±0.03ppm in Dorsanum miran and nickel 0.81±0.50 ppm in Penaeus notialis. Conclusion: The microbial loads of the seafood are significantly (p≤0.05) higher in gastropods and more than acceptable standard, effective protection of water bodies for food cultivation and constant monitoring of seafood is necessary. Adequate processing and employing good manufacturing practices can reduce the microbial loads to an acceptable level and prevent food borne hazards that could be associated with seafood consumption. Keywords: Seafood; Heavy metals; Microbial profile; proximate compositions ÉTUDE COMPARATIVE DE LA PROLIFÉRATION MICROBIENNE, PROCHAINE ET HEAVY METAL COMPOSITIONS DE CERTAINS GASTÉROPODES, MOLLUSQUES ET CRUSTACÉS LES FRUITS DE MER

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Section: Discussionsupporting

confidence: 91%

Comparative study of microbial, proximate and heavy metal compositions of some gastropods, bivalve and crustacean seafood

Oranusi¹,

Effiong²,

Duru³

2018

Af J Clin Exp Micro

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“…Correlation between EC and microbial levels was, however, not specifically evaluated since the clams used were depurated. Ekanem and Adegoke (1995) reported a 97% reduction in the bacterial load of these clams during depuration. In general, however, good correlation (0.85<r<0.9) exists between EC and the bacteriological quality of fish (Gibson 1985).…”

Section: Results Inmentioning

confidence: 91%

“…Unfortunately, this (boiling) is the most widely used method of shucking molluscs in Nigeria and many other developing countries. Considering the prevailing poor sanitary state of molluscan growing-waters in Nigeria which indicate that these shellfish carry untolerable levels of pathogens (Ekanem and Adegoke 1995;Ekanem and Otti 1997), the product could be sanitized by boiling. The benefit of this will, however, depend largely on minimizing contamination during further processing and storage of the meat.…”

Section: Resultsmentioning

confidence: 99%

EFFECTS of SHUCKING METHOD ON OPENING, MEAT YIELD and SELECTED QUALITY PARAMETERS of WEST AFRICAN CLAM, GALATEA PARADOXA (BORN)

Ekanem

Achinewhu

2000

J Food Processing Preservation

Self Cite

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Samples (n= 100) of freshly harvested clams (Galatea paradoxa Born) from the Cross River, Nigeria, were subjected after 24 h depurations to heat treatment (steam and water at 60, 70, 80, 90, 100C) for 1–6 min to evaluate the effects of level of heat treatment on opening, meat yield, sensory properties, proximate composition, pH and electrical conductivity (EC). Observations were also made on the effects of some chemical shucking aids (NaOH, NaHCO3, Na2CO3, NaCl) in 60C water on these parameters. Results showed that boiling water was most effective in opening the clams, with 100% shucking achieved in 1 min. Steam was least effective, requiring 6 min for 100% opening. Temperature significantly and strongly influenced meat yield (p<0.05;r=‐0.92). pH (p<0.01; r=0.97), EC (p<0.05; r =0.65) and sensory properties (p<0.05). In general, shucking aids reduced opening time, significantly p<0.05) raised meat pH and EC, and with the exception of NaCl, insignificantly (P>0.05) improved yield. NaHCO3, and Na2CO3, which cut time for 100% opening from 5 min to 2 min were most effective. There were slight but significant (P <0.05) drops in meat moisture, crude protein and ash contents with increase in temperature (T). the model equations, pH = 4.69 + 0.021 T and % yield = 39.95–0.172 T were found to reliably predict meat pH and yield, with insignificant differences (P>0.05) between predicted and experimental values.

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“…radula are harvested are contaminated and since the periwinkles are filter feeders there is a tendency that they will accumulate high levels of pathogens as a result of cross contamination. Ekanem and Adegoke [25] stated that the level of pollution of the cultivation waters determines the level of contamination of shellfish. The presence of enteric organisms in the presence study is an indication of pollution of their underlying waters with untreated faecal waste and sewage.…”

Section: Discussionmentioning

confidence: 99%

Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

Asemota¹,

Makut

Obiekezie

et al. 2020

SAJRM

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The aim of this study was to determine the antibiogram of bacterial isolates from Tympanotonus fuscatus var. radula sold in markets in Nasarawa State. Nigeria. Samples of Tympanotonus fuscatus var. radula (periwinkles) were bought from soup ingredient sellers at different sale locations in Keffi, Masaka and Orange markets and were analyzed using standard bacteriological methods. The bacterial isolates were identified using morphological, cultural and biochemical techniques. The total bacteria count varied from 1.18–3.20 x 108 CFU/g for the raw samples while the total bacterial count for the boiled samples varied from 0–1.57 x 108 CFU/g. Periwinkle samples with shells from Masaka market had the highest bacterial load with a mean total bacterial count of 2.94 x 10⁸ CFU/g and mean total coliform count of 2.80 x 10⁶ CFU/g. Raw periwinkle samples with shells had a higher bacterial load than samples without shells. There was also a drastic reduction in the bacterial load in the periwinkle samples after boiling under laboratory conditions. The bacteria isolated were Bacillus spp. and Staphylococcus aureus were the Gram-positive bacteria isolated. Enterobacter spp., Escherichia coli, Salmonella spp., Pseudomonas spp., Serratia spp. and Proteus spp. The most frequently occurring gram positive bacteria was Escherichia coli with an isolation frequency of 6(24%), the least frequently occurring was Bacillus spp., 8(32)%. Antibiotic susceptibility test showed that all the gram negative organisms exhibited sensitivity to ciprofloxacin: Escherichia coli (32 mm), Enterobacter spp. (41.5 mm), Proteus spp. (40.0 mm), Salmonella spp. (37.0 mm), Serratia spp. (26.0 mm), Pseudomonas spp. (23.0 mm). All the gram negative organisms showed marked resistance to vancomycin: Escherichia coli (12.0 mm), Enterobacter spp. (10.0 mm), Proteus spp. (11.0 mm), Salmonella spp. (5.0 mm), Serratia spp. (10.0 mm) and Pseudomonas spp. (4.5 mm).

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Bacteriological study of West African clam (Egeria radiata Lamarch) and their overlying waters

Cited by 20 publications

References 10 publications

Comparative study of microbial, proximate and heavy metal compositions of some gastropods, bivalve and crustacean seafood

Comparative study of microbial, proximate and heavy metal compositions of some gastropods, bivalve and crustacean seafood

EFFECTS of SHUCKING METHOD ON OPENING, MEAT YIELD and SELECTED QUALITY PARAMETERS of WEST AFRICAN CLAM, GALATEA PARADOXA (BORN)

Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

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