Baculovirus transduction of rat articular chondrocytes: roles of cell cycle

Lee, Hsiao-Ping; Chen, Yen‐Lin; Shen, Heng-Chun; Lo, Wen-Hsin; Hu, Yu‐Chen

doi:10.1002/jgm.994

Cited by 26 publications

(21 citation statements)

References 44 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell cycle plays an important role in the influence on division and duplication of chondrocyte, which can be divided into four phases: G 1 (gap 1), S (synthesis), during which DNA is replicated, G 2 and M (mitosis), in which cell division occurs (19,20). The DNA content of cells is a constant parameter that varies over the progression of the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition

et al. 2012

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Millimeter waves, high-frequency electromagnetic waves, can effectively alleviate the clinical symptoms in osteoarthritis patients, as a non-pharmaceutical and non-invasive physical therapy regimen. However, the molecular mechanisms of the therapeutic effects of millimeter wave treatment are not well understood. In the present study, the effect of millimeter waves on the G 1 /S cell cycle progression in chondrocytes and the underlying mecha nism was investigated. Chondrocytes isolated from the knee of SD rats were cultured and identified using toluidine blue staining. The second generation chondrocytes were collected and stimulated with or without millimeter waves for 48 h. Chondrocyte viability was analyzed using the MTT assay. The cell cycle distribution of chondrocytes was analyzed by flow cytometry. mRNA and protein expression levels of cyclin D1, cyclin-dependent kinases 4 and 6 (CDK4 and CDK6) and p21 were detected using real-time PCR and western blotting, respectively. Millimeter wave stimulation was found to significantly enhance chondrocyte viability. Moreover, the percentage of chondrocytes in the G 0 /G 1 phase was significantly decreased, whereas that in the S phase was significantly increased. In addition, following millimeter wave treatment, cyclin D1, CDK4 and CDK6 expression was significantly upregulated, whereas p21 expression was significantly downregulated. The results indicate that millimeter wave treatment promotes chondrocyte proliferation via cell cycle progression. IntroductionOsteoarthritis (OA), the most frequent arthropathy, is characterized by progressive degradation of hyaline articular cartilage and is associated with aging (1). A complex interplay of mechanical, and functional changes of chondrocytes could precipitate the imbalance between matrix anabolic and catabolic processes in articular cartilage and thus the pathogenesis of OA (2,3). Chondroctyes can rapidly respond to changes in the articular microenvironment and regulate the dynamic equilibrium between the anabolism and catabolism of the extracellular matrix (ECM), which plays a crucial role in the maintenance of the cartilage function (4). Therefore, enhancing chondrocyte function might be an efficient treatment to cure or even delay the progression of OA by promoting chondrocyte proliferation.The cell cycle plays an important role in the influence on chondrocyte function, which takes place in chondrocytes leading to its division and duplication. Cell cycle control is a highly regulated process that involves a complex cascade of events, in which regulation can be realized through a complicated network by cyclins, cyclin-dependent kinases (CDKs) and cyclin dependent kinase inhibitors (CDKI). G 1 /S transition, one of the two main checkpoints, is a rate-limiting step in the cell cycle and regulates cell proliferation (5,6). Cyclin D1 is a key cell cycle regulatory protein, which binds to CDK4 or CDK6 to control cell cycle progression from the G 1 to the S phase (7). Meanwhile, p21 competes with cyclin D1 for bindin...

show abstract

Section: Discussionmentioning

confidence: 99%

Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition

et al. 2012

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…The cell cycle plays an important role in the division and duplication of chondrocytes and can be divided into 4 phases: G1 phase, preparation for DNA synthesis; S phase, DNA synthesis; G2 phase, preparation for mitosis; and M phase, mitosis (31), where cell division occurs (32). The G1/S transition is one of the 2 main checkpoints used by a cell to regulate the progression of the cell cycle and following that, chondrocytes can pass through to the S phase and complete cell division (33).…”

Section: Discussionmentioning

confidence: 99%

Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

Chen

Fan

et al. 2013

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Duhuo Jisheng Decoction (DHJSD), a well known traditional Chinese folk medicine, is used for eliminating stagnation, removing blood stasis, promoting blood circulation and alleviating pain; it is commonly used for the treatment of various diseases, including osteoarthritis (OA). However, the molecular mechanisms behind the therapeutic effects of OA remain unclear. In the present study, the effects of DHJSD on the morphology of articular cartilage and the G1/S cell cycle progression in chondrocytes, as well as the underlying mechanisms, were investigated. A total of 27 two-month-old male Sprague Dawley rats were randomly divided into 3 groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9.3 g/kg/day)]. After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscope and by transmission electron microscopy (TEM) and the mRNA and protein expression levels of cyclin D1, CDK4, CDK6, retinoblastoma protein (Rb) and p16 were measured by RT-PCR and immunohistochemistry, respectively. Treatment with DHJSD significantly improved the arrangement of collagen fibers in the articular cartilage, as well as its structure and reduced cell degeneration compared with the model group. The mRNA and protein expression levels of cyclin D1, CDK4, CDK6 and Rb in the DHJSD-treated group were significantly increased compared with those in the model group, whereas p16 expression was significantly downregulated. Taken together, these results indicate that DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by upregulating the expression of cyclin D1, CDK4, CDK6 and Rb and downregulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis.

show abstract

“…Instead, the varied expression levels is attributed to the rapidly altering cellular transcription machinery in the course of differentiation progression [36]. Besides, baculovirus transduction of rat articular chondrocytes is dependent on the cell cycle [37]. The chondrocytes predominantly in G 2 /M phase are approximately twice as efficient in the transgene (egfp) expression as the cycling cells, while the cells in S and G 1 phases express egfp as efficiently as the cycling cells.…”

Section: B Factors Influencing Baculovirus-mediated Expressionmentioning

confidence: 99%

“…The chondrocytes predominantly in G 2 /M phase are approximately twice as efficient in the transgene (egfp) expression as the cycling cells, while the cells in S and G 1 phases express egfp as efficiently as the cycling cells. However, the chondrocyte population rich in quiescent cells results in poorer transgene expression due to less effective nuclear transport of baculoviral DNA and higher degree of methylation [37].…”

Section: B Factors Influencing Baculovirus-mediated Expressionmentioning

confidence: 99%

Baculoviral Vectors for Gene Delivery: A Review

2008

CGT

Self Cite

132

View full text Add to dashboard Cite

Baculovirus has emerged as a novel vector for in vivo and in vitro gene delivery. In addition, the applications of baculovirus-mediated gene transfer have been explosively expanded to drug screening, eucaryotic gene display, cancer therapy and tissue engineering, etc. The capability of baculovirus viral envelope for protein/peptide display also renders itself a potential vaccine delivery platform. This paper reviews the history, factors influencing baculovirus-mediated gene delivery and emerging in vitro, in vivo and ex vivo applications. Efforts aimed at overcoming current existing bottlenecks and recent progresses in addressing the safety concerns are particularly emphasized.

show abstract

Baculovirus transduction of rat articular chondrocytes: roles of cell cycle

Abstract: These findings unravel the practical limitations when employing baculovirus in cartilage tissue engineering. The implications and possible solutions are discussed.

Cited by 26 publications

References 44 publications

Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition

Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition

Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

Baculoviral Vectors for Gene Delivery: A Review

Contact Info

Product

Resources

About