Baicalin attenuates oxygen-glucose deprivation-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase activation in PC12 cells

Abstract

Aim: To determine whether the flavonoid baicalin attenuates oxygen-glucose deprivation (OGD)-induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase (5-LOX) activation in PC12 cells. Methods: The effects of baicalin and the 5-LOX inhibitor zileuton on the changes induced by OGD/recovery or H 2 O 2 (an exogenous reactive oxygen species [ROS]) in green fluorescent protein-5-LOX-transfected PC12 cells were compared. Results: Both baicalin and zileuton attenuated OGD/recovery-and H 2 O 2 -induced injury and inhibited OGD/recovery-induced production of 5-LOX metabolites (cysteinyl leukotrienes) in a concentration-dependent manner. However, baicalin did not reduce baseline cysteinyl leukotriene levels. Baicalin also reduced OGD/recovery-induced ROS production and inhibited 5-LOX translocation to the nuclear envelope and p38 phosphorylation induced by OGD/recovery and H 2 O 2 . In contrast, zileuton did not show these effects. Conclusion: Baicalin can inhibit 5-LOX activation after ischemic injury, which may partly result from inhibition of the ROS/p38 mitogenactivated protein kinase pathway.

Baicalin prevents the production of hydrogen peroxide and oxidative stress induced by Aβ aggregation in SH-SY5Y cells

“…Together with the neuroprotective effects of baicalin reported by others [7,11,12,19,21,30,31], this study provides further supportive evidence for the potential use of baicalin as a therapeutic agent to reduce A␤-induced neuronal cytotoxicity. It is possible that baicalin may be beneficial to prevent or treat AD acting through the aforementioned mechanisms, however, further in vivo studies are needed to determine whether baicalin or its metabolites can indeed be transported across the blood brain barrier to reach brain tissue and accumulate up to a level for performing such functions, as the existing evidence of baicalin absorption and tissue distribution data published to date for baicalin are inconclusive [3,13,18,30,33].…”

Suppression of Oxidative Stress and 5-Lipoxygenase Activation by Edaravone Improves Depressive-Like Behavior after Concussion

“…35 It was recently reported that zileuton, another 5-LOX inhibitor with the same mechanism of action as BWB70C, does not suppress 5-LOX translocation to the nuclear envelope after oxygenglucose deprivation. 23,36 This is considered to provide reasonable explanation on the reason why BWB70C could not inhibit the 5-LOX translocation in our experiments. Therefore, these results suggest that concussion-induced 5-LOX activation in hippocampal astrocytes is associated with the appearance of depressive-like behavior.…”

β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy

“…It can acquire neuron-like properties when exposed to nerve growth factor (NGF) [12] . Therefore, it is widely used as an in vitro model of cerebral ischemia/reperfusion [13,14] . Intracellular free calcium concentration ([Ca 2+ ] i ) and mitochondrial membrane potential (MMP) are important indicators to reflect cell injury.…”