Baicalin modulates microRNA expression in UVB irradiated mouse skin

Xu, Yang; Zhou, Bingrong; Wu, Di; Yin, Zhiqiang; Luo, Dan

doi:10.1016/s1674-8301(12)60022-0

Cited by 33 publications

(30 citation statements)

References 30 publications

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“…Additionally, recent studies have implicated novel roles of miRNAs in the regulation of the autophagy process [22, 23]. Our group previously found that miR-23a was up-regulated during UVB irradiation in mouse epidermis and keratinocytes, as well as human keratinocytes (cell line HaCaT) cells [13, 24, 25]. However, miR-23a has also been found to function as a growth-promoting and anti-apoptotic factor in hepatocellular carcinoma and gastric adenocarcinoma cell lines [26, 27].…”

Section: Discussionmentioning

confidence: 99%

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

Zhang

Zhou

et al. 2016

Oncotarget

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Autophagy is a cellular catabolic mechanism that is activated in response to stress conditions, including ultraviolet (UV) irradiation, starvation, and misfolded protein accumulation. Abnormalities in autophagy are associated with several pathologies, including aging and cancer. Furthermore, recent studies have demonstrated that microRNAs (miRNAs) are potent modulators of the autophagy pathway. As a result, the current study aims to elucidate the role of the autophagy-related miRNA miR-23ain the process of photoaging. Experiments demonstrated that the antagomir-mediated inactivation of miR-23a resulted in the stimulation of PUVA- and UVB-depressed autophagy flux and protected human fibroblasts from premature senescence. Furthermore, AMBRA1 was identified as a miR-23a target. AMBRA1 cellular levels increased following the introduction of miR-23a antagomirs. And a bioinformatics analysis revealed that the AMBRA1 3′ UTR contains functional miR-23a responsive sequences. Finally, it was also demonstrated that both AMBRA1 overexpression and Rapamycin treatment were both able to rescue fibroblasts from PUVA and UVB irradiation-induced autophagy inhibition, but that these effects could also be mitigated by miR-23a overexpression. Therefore, this study concludes that miR-23a-regulated autophagy is a novel and important regulator of ultraviolet-induced premature senescence and AMBRA1 is a rate-limiting miRNA target in this pathway.

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Section: Discussionmentioning

confidence: 99%

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

Zhang

Zhou

et al. 2016

Oncotarget

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“…Hsa-miR-532-5p regulates also stanniocalcin 2 (STC2): it is a glycoprotein hormone that plays an important role in calcium and phosphate homeostasis and is considered a tumor progression predictor for gastric cancer [119] and breast carcinoma [120]. Concerning Hsa-miR-188-5p, it already results to be overexpressed in UVB irradiated mouse skin [121], suggesting a potential role of this miR in response to oxidative stress linked to radiation. As oxidative stress response is one of the active pathways of tumor cells (see [122] for a review), thus this miRNA is expected to be up-regulated in G3 tumor samples.…”

Section: Discussionmentioning

confidence: 99%

Integration of mRNA Expression Profile, Copy Number Alterations, and microRNA Expression Levels in Breast Cancer to Improve Grade Definition

et al. 2014

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Defining the aggressiveness and growth rate of a malignant cell population is a key step in the clinical approach to treating tumor disease. The correct grading of breast cancer (BC) is a fundamental part in determining the appropriate treatment. Biological variables can make it difficult to elucidate the mechanisms underlying BC development. To identify potential markers that can be used for BC classification, we analyzed mRNAs expression profiles, gene copy numbers, microRNAs expression and their association with tumor grade in BC microarray-derived datasets. From mRNA expression results, we found that grade 2 BC is most likely a mixture of grade 1 and grade 3 that have been misclassified, being described by the gene signature of either grade 1 or grade 3. We assessed the potential of the new approach of integrating mRNA expression profile, copy number alterations, and microRNA expression levels to select a limited number of genomic BC biomarkers. The combination of mRNA profile analysis and copy number data with microRNA expression levels led to the identification of two gene signatures of 42 and 4 altered genes (FOXM1, KPNA4, H2AFV and DDX19A) respectively, the latter obtained through a meta-analytical procedure. The 42-based gene signature identifies 4 classes of up- or down-regulated microRNAs (17 microRNAs) and of their 17 target mRNA, and the 4-based genes signature identified 4 microRNAs (Hsa-miR-320d, Hsa-miR-139-5p, Hsa-miR-567 and Hsa-let-7c). These results are discussed from a biological point of view with respect to pathological features of BC. Our identified mRNAs and microRNAs were validated as prognostic factors of BC disease progression, and could potentially facilitate the implementation of assays for laboratory validation, due to their reduced number.

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“…Recently, p63-miRNA feedback was identified as an important signaling pathway in keratinocyte senescence (35). Another study determined that the protective effect of baicalin on UVB-treated keratinocytes was mediated by miRNA expression modulation (36), indicating that miRNAs have important roles in keratinocyte proliferation. Therefore, our miRNA-based study regarding keratinocytes may provide important information regarding anti-UV therapeutics.…”

Section: Discussionmentioning

confidence: 99%

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

Choe

et al. 2012

International Journal of Molecular Medicine

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Abstract. This study aimed to evaluate the protective effects of Centella asiatica (C. asiatica) against ultraviolet B (UVB) damage in human keratinocytes using microRNA (miRNA) expression profiling analysis. Titrated extract of C. asiatica (TECA) demonstrated low cytotoxicity in normal human HaCaT keratinocytes only at low doses (<5 µg/ml). UVB (50 mJ/cm 2 ) irradiation significantly decreased cell viability, and TECA treatment decreased the UVB toxicity. By using miRNA microarrays, we determined that 72 miRNAs had an altered expression following TECA treatment in UVB-irradiated keratinocytes (46 upregulated and 26 downregulated). Using an miRNA target gene prediction tool and Gene Ontology (GO) analysis, we determined that miRNAs with altered expression were functionally related with the inhibition of apoptosis and cell proliferation. Overall, these results provide meaningful information to facilitate the understanding of TECA-mediated UVB protection in human keratinocytes.

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Baicalin modulates microRNA expression in UVB irradiated mouse skin

Cited by 33 publications

References 30 publications

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

Integration of mRNA Expression Profile, Copy Number Alterations, and microRNA Expression Levels in Breast Cancer to Improve Grade Definition

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

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