Baicalin modulates NF-κB and NLRP3 inflammasome signaling in porcine aortic vascular endothelial cells Infected by Haemophilus parasuis Causing Glässer’s disease

Fu, Shulin; Liu, Huashan; Xu, Lei; Qiu, Yinsheng; Liu, Yu; Wu, Zhongyuan; Yuan, Chun; Hou, Yongqing; Hu, Chien‐An Andy

doi:10.1038/s41598-018-19293-2

Cited by 35 publications

(22 citation statements)

References 76 publications

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“…PAVECs were isolated, identified, and cultured as described previously, with minor modifications [ 52 , 53 ]. PAVECs were obtained in small sheets after treatment of aortic lumen (30 min, 37 °C) with 0.1% type I collagenase (Sigma, St. Louis, MO, USA) in M-199 medium (Gibco, New York, NY, USA) containing penicillin–streptomycin solution (Gibco).…”

Section: Methodsmentioning

confidence: 99%

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis

Guo

et al. 2018

IJMS

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Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated. The 281 DEGs were involved in 136 KEGG signaling pathways that were organismal systems, environmental information processing, metabolism, cellular processes, and genetic information processing. The main pathways were the Rap1, FoxO, and PI3K/Akt signaling pathways, and the overexpressed genes were determined and verified by quantitative reverse transcription polymerase chain reaction. In addition, 252 genes were clustered into biological processes, molecular processes, and cellular components. Our study provides new insights for understanding the interaction between bacterial and host cells, and analyzed, in detail, the possible mechanisms that lead to vascular damage induced by H. parasuis. This may lead to development of novel therapeutic targets to control H. parasuis infection.

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Section: Methodsmentioning

confidence: 99%

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis

Guo

et al. 2018

IJMS

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“…Here, we collect natural telomerase inhibitors reported in the literature and summarize their telomerase inhibition effects, structures, possible mechanisms, and provide references for pharmaceutical chemists searching for suitable lead compounds. Table and Figures and show natural products and chemical structures that target the telomere elongation process …”

Section: Small Molecule Inhibitorsmentioning

confidence: 99%

Therapeutic strategies for targeting telomerase in cancer

Chen

Tang

Shi

et al. 2019

Medicinal Research Reviews

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Telomere and telomerase play important roles in abnormal cell proliferation, metastasis, stem cell maintenance, and immortalization in various cancers. Therefore, designing of drugs targeting telomerase and telomere is of great significance. Over the past two decades, considerable knowledge regarding telomere and telomerase has been accumulated, which provides theoretical support for the design of therapeutic strategies such as telomere elongation. Therefore, the development of telomere‐based therapies such as nucleoside analogs, non‐nucleoside small molecules, antisense technology, ribozymes, and dominant negative human telomerase reverse transcriptase are being prioritized for eradicating a majority of tumors. While the benefits of telomere‐based therapies are obvious, there is a need to address the limitations of various therapeutic strategies to improve the possibility of clinical applications. In this study, current knowledge of telomere and telomerase is discussed, and therapeutic strategies based on recent research are reviewed.

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“…We have previously demonstrated that the anti‐inflammatory effects of baicalin are mediated through the suppression of the NLRP3 inflammasome pathway in a lipopolysaccharide (LPS)‐challenged piglet mononuclear phagocyte model (Ye et al, ). Baicalin also suppresses the secretion of the inflammatory cytokines and HMGB1 via NF‐κB and NLRP3 signaling in Haemophilus parasuis (HPS)‐stimulated piglet cells (Fu, Liu, Chen, et al, ; Fu, Liu, Xu, et al, ; Fu et al, ). Furthermore, baicalin can reverse apoptosis initiated by HPS by altering PKC–MAPK signaling (Ye et al, ).…”

Section: Pharmacokinetic Parameters Of Baicalin Following Single Intrmentioning

confidence: 99%

Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

Liu

Zhao

et al. 2019

Vet Pharm & Therapeutics

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The purpose of this study was to determine the pharmacokinetics of baicalin after intravenous and intramuscular administration of sodium baicalin at 50 mg/kg to piglets. Plasma baicalin levels were determined by high‐performance liquid chromatography. The plasma concentration–time data of baicalin for both administration routes were best described by two‐compartmental open model. The area under the plasma concentration–time curve and the elimination half‐lives were 77.47 ± 6.14 µg/ml × h and 1.73 ± 0.16 hr for intravenous and 64.85 ± 5.67 µg/ml × h and 2.42 ± 0.15 hr for intramuscular administration, respectively. The apparent volume of distribution and body clearance were 1.63 ± 0.23 L/kg and 2.74 ± 0.30 L h−1 kg−1 for intravenous and 0.51 ± 0.10 L/kg and 0.78 ± 0.08 L h−1 kg−1 for intramuscular routes, respectively. An intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 ± 7.40 µg/ml at 0.17 hr and a high absolute bioavailability of 83.73 ± 5.53%.

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Baicalin modulates NF-κB and NLRP3 inflammasome signaling in porcine aortic vascular endothelial cells Infected by Haemophilus parasuis Causing Glässer’s disease

Cited by 35 publications

References 76 publications

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis

Therapeutic strategies for targeting telomerase in cancer

Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

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