Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model

Pimentel, Leticia Santos; Turini, Carolina Alvarenga; Santos, Paula de Souza; Morais, Mariana Abilio de; Souza, Aline Gomes de; Barbosa, Mariana Botelho; Martins, Estefânia Mara do Nascimento; Coutinho, Loyane Bertagnolli; Furtado, Clascídia Aparecida; Ladeira, Luiz Orlando; Martins, João Ricardo; Goulart, Luíz Ricardo; Faria, Paulo Eustáquio de

doi:10.1016/j.nano.2019.102137

Cited by 11 publications

(8 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering that CP-PEI and CP-PEI-Zn possessed a similar particle size, composition, and antigen loading capacity, the better effect of CP-PEI-Zn in enhancing cellular immunity may result from the different antigen adsorption modes of these two particles, which may, in turn, affect the immunogenicity of the antigen from the following aspects. First, although antigens formulated in particulate adjuvants can be more efficiently taken up than the soluble antigen by APCs to stimulate stronger cellular immune responses, particles with better dispersibility are reported to induce a stronger cell-mediated immune response . As shown in Figure S2, compared to 146S@CP-PEI, the 146S@CP-PEI-Zn exhibited significantly improved dispersibility and colloidal stability.…”

Section: Resultsmentioning

confidence: 90%

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

Yang

Lin

et al. 2020

Mol. Pharmaceutics

View full text Add to dashboard Cite

The interactions between antigen and adjuvant were among the most significant factors influencing the immunogenicity of vaccines, especially for unstable antigens like inactivated foot and mouth disease virus (iFMDV). Here we propose a novel antigen delivery pattern based on the coordination interaction between transition metal ions Zn2+ chelated to chitosan nanoparticles and iFMDV, which is known to be rich in histidine. The zinc chelated chitosan particles (CP-PEI-Zn) were prepared by cross-linking chitosan particles (CP) with sodium tripolyphosphate (TPP), modifying with metal chelator polyethylenimine (PEI), and subsequent chelating of Zn2+. The coordination interaction was confirmed by analyzing the adsorption and desorption behavior of iFMDV on CP-PEI-Zn by high-performance size exclusion chromatography (HPSEC), while the CP-PEI without chelating Zn2+ loads iFMDV mainly through electrostatic interactions. The iFMDV loaded on CP-PEI-Zn showed better thermal stability than that on CP-PEI, as revealed by a slightly higher transition temperature (T m) related to iFMDV dissociation. After subcutaneous immunization in female Balb/C mice, antigens loaded on CP-PEI and CP-PEI-Zn all induced higher specific antibody titers, better activation of B lymphocytes, and more effector-memory T cells proliferation than the free antigen and iFMDV adjuvanted with ISA 206 emulsion did. Moreover, CP-PEI-Zn showed superior efficacy to CP-PEI in promoting the proliferation of effector-memory T cells and secretion of cytokines, indicating a more potent cellular immune response. In summary, the CP-PEI-Zn stabilized the iFMDV after loading and promoted both humoral and cellular immune responses, thus reflecting its potential to be a promising adjuvant for the iFMDV vaccine and other unstable viral antigens.

show abstract

Section: Resultsmentioning

confidence: 90%

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

Yang

Lin

et al. 2020

Mol. Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The poly-anhydrides encapsulated whole cell lysates and Mycobacterium paratuberculosis culture filtrates by nanoprecipitation and proved to be very safe. Both nanovaccines provided more effective, homogeneous, and stable responses than commercial ruminant vaccines (peptide adjuvanted Anaplasmosis or inactivated Johne’s disease vaccines) opening up new vaccine perspectives for the control of intracellular pathogens [ 164 , 165 ]. Polyanhydride formulations including a lipopolysaccharide O-antigen-deficient rough Brucella abortus mutant from a virulent field strain have been tested for extended antigen release in cattle via implants to mimic the kinetics of antigen availability during persistent infection.…”

Section: Latest Nanovaccine Applications Regarding One Health Relevant Pathogensmentioning

confidence: 99%

Nanovaccines against Animal Pathogens: The Latest Findings

et al. 2021

View full text Add to dashboard Cite

Nowadays, safe and efficacious vaccines represent powerful and cost-effective tools for global health and economic growth. In the veterinary field, these are undoubtedly key tools for improving productivity and fighting zoonoses. However, cases of persistent infections, rapidly evolving pathogens having high variability or emerging/re-emerging pathogens for which no effective vaccines have been developed point out the continuing need for new vaccine alternatives to control outbreaks. Most licensed vaccines have been successfully used for many years now; however, they have intrinsic limitations, such as variable efficacy, adverse effects, and some shortcomings. More effective adjuvants and novel delivery systems may foster real vaccine effectiveness and timely implementation. Emerging vaccine technologies involving nanoparticles such as self-assembling proteins, virus-like particles, liposomes, virosomes, and polymeric nanoparticles offer novel, safe, and high-potential approaches to address many vaccine development-related challenges. Nanotechnology is accelerating the evolution of vaccines because nanomaterials having encapsulation ability and very advantageous properties due to their size and surface area serve as effective vehicles for antigen delivery and immunostimulatory agents. This review discusses the requirements for an effective, broad-coverage-elicited immune response, the main nanoplatforms for producing it, and the latest nanovaccine applications for fighting animal pathogens.

show abstract

“…However, purification of OM proteins is cost prohibiting, therefore, the use of subunit vaccines in combination with novel delivery platforms is particularly promising. Nano platforms have been explored in A. marginale vaccines including silica vesicles (SV100) carrying type IV secretion system proteins (T4SS) VirB9-1 and VirB9-2 (Zhao et al, 2016;Zhao L. et al, 2017), and VirB9-1 and VirB10 proteins (Zhang et al, 2016), as well as carbon nanotubes carrying the major surface protein (MSP)1a (Pimentel et al, 2020), in the mouse model. These formulations induce both cell-mediated and humoral responses that are protective against challenge; however, they have not been tried in cattle.…”

Section: Bacterial Diseasesmentioning

confidence: 99%

Applications of Nanovaccines for Disease Prevention in Cattle

Maina

Grego

Boggiatto

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Vaccines are one of the most important tools available to prevent and reduce the incidence of infectious diseases in cattle. Despite their availability and widespread use to combat many important pathogens impacting cattle, several of these products demonstrate variable efficacy and safety in the field, require multiple doses, or are unstable under field conditions. Recently, nanoparticle-based vaccine platforms (nanovaccines) have emerged as promising alternatives to more traditional vaccine platforms. In particular, polymer-based nanovaccines provide sustained release of antigen payloads, stabilize such payloads, and induce enhanced antibod- and cell-mediated immune responses, both systemically and locally. To improve vaccine administrative strategies and efficacy, they can be formulated to contain multiple antigenic payloads and have the ability to protect fragile proteins from degradation. Nanovaccines are also stable at room temperature, minimizing the need for cold chain storage. Nanoparticle platforms can be synthesized for targeted delivery through intranasal, aerosol, or oral administration to induce desired mucosal immunity. In recent years, several nanovaccine platforms have emerged, based on biodegradable and biocompatible polymers, liposomes, and virus-like particles. While most nanovaccine candidates have not yet advanced beyond testing in rodent models, a growing number have shown promise for use against cattle infectious diseases. This review will highlight recent advancements in polymeric nanovaccine development and the mechanisms by which nanovaccines may interact with the bovine immune system. We will also discuss the positive implications of nanovaccines use for combating several important viral and bacterial disease syndromes and consider important future directions for nanovaccine development in beef and dairy cattle.

show abstract

Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model

Cited by 11 publications

References 55 publications

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

Nanovaccines against Animal Pathogens: The Latest Findings

Applications of Nanovaccines for Disease Prevention in Cattle

Contact Info

Product

Resources

About

Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model

Cited by 11 publications

References 55 publications

A Novel Particulate Delivery System Based on Antigen–Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

A Novel Particulate Delivery System Based on Antigen–Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

Nanovaccines against Animal Pathogens: The Latest Findings

Applications of Nanovaccines for Disease Prevention in Cattle

Contact Info

Product

Resources

About

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus

A Novel Particulate Delivery System Based on Antigen–Zn²⁺ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus