BALF-derived fibroblasts differ from biopsy-derived fibroblasts in systemic sclerosis

Scheja, Agneta; Larsen, Kristoffer; Todorova, Lizbet; Tufvesson, Ellen; Wildt, Marie; Åkesson, Anita; Hansson, Lennart; Ellis, Samantha; Thorsson, G Westergren

doi:10.1183/09031936.00135205

Cited by 23 publications

(22 citation statements)

References 26 publications

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“…The latter hypothesis is consistent with observations made in asthma or systemic sclerosis, where alveolar fibroblasts and interstitial fibroblasts obtained from the same patient exhibited different phenotypes [30][31][32]. We were not able to perform such a comparison as obtaining fibroblasts from BAL and biopsy in the same patient with ALI/ARDS was not possible in the clinical setting for ethical concerns.…”

Section: Critical Care and Lung Injury C Quesnel Et Alsupporting

confidence: 68%

Alveolar fibroblasts in acute lung injury: biological behaviour and clinical relevance

Quesnel¹,

Nardelli²,

Piednoir³

et al. 2009

Eur Respir J

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Although fibroblasts are key cells in the lung repair/fibrosis process, their characteristics are poorly studied in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). The aims of our study were to: 1) determine the biological behaviour of alveolar fibroblasts during ALI; and 2) to evaluate the clinical relevance of positive alveolar fibroblast culture from patients with ALI/ARDS. Cells were cultured from bronchoalveolar lavage (BAL) obtained from 68 critically ill, ventilated patients: ALI n517; ARDS n531; and ventilated controls n520. Patients were followed for 28 days and clinical data was recorded. We studied proliferation, migration and collagen-1 synthesis capacities of fibroblasts.Cells expressing fibroblast markers were cultured from BAL obtained in six (35%) ALI patients and six (19%) ARDS patients, but never from ventilated controls. Alveolar fibroblasts exhibited a persistent activated phenotype with enhanced migratory and collagen-1 production capacities, with hyporesponsiveness to prostaglandin E 2 compared to normal lung fibroblasts (pf0.04). Positive fibroblast culture was associated with both an increased collagen-1 concentration and monocyte/macrophage percentage in BAL fluid (pf0.01), and with a reduced duration of mechanical ventilation (p,0.001).We conclude that activated alveolar fibroblasts can be cultured either in ALI or ARDS and that their presence might reflect the initiation of the organising phase of ALI.

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Section: Critical Care and Lung Injury C Quesnel Et Alsupporting

confidence: 68%

Alveolar fibroblasts in acute lung injury: biological behaviour and clinical relevance

Quesnel¹,

Nardelli²,

Piednoir³

et al. 2009

Eur Respir J

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“…13,17,18 This indicates that versican is most likely a marker not only for BOS but is also for pathologic wound healing processes. One major function of versican is to bind to hyaluronan, and it also has the capacity to absorb water molecules.…”

Section: Discussionmentioning

confidence: 96%

Fibroblast phenotypes and their activity are changed in the wound healing process after lung transplantation

Andersson-Sjöland

Thiman

Nihlberg

et al. 2011

The Journal of Heart and Lung Transplantation

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“…In addition, in previous clinical studies of SSc-ILD, the increased percentage of eosinophils in bronchoalveolar lavage fluid (BALF) was associated with decreased lung diffusing capacity for carbon monoxide and increased mortality (Bouros et al 2002; Silver and Clements 2003). Hence, the inclusion criteria of patients in an important clinical trial yielded evidence of increased percentages of eosinophils in BALF (Tashkin et al 2006), and an outgrowth of fibroblasts was observed in patients with an increased percentage of eosinophils in BALF (Scheja et al 2007). In addition, in patients with bronchial asthma, we previously reported that the concentration of ECP and eosinophil-protein X (EPX) in sputum were higher than and significantly correlated with those in serum (Motojima et al 1995; Koseki et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Associations between peripheral blood eosinophil counts in patients with systemic sclerosis and disease severity

et al. 2016

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Increased levels of serum pro-fibrotic cytokines have been reported in patients with systemic sclerosis (SSc). Some of these cytokines also play an important role in the differentiation and migration of eosinophils. The aim of this study was to determine whether eosinophilic inflammation is caused in SSc. We retrospectively reviewed the peripheral blood eosinophil counts in 70 untreated patients with SSc and compared them with those in patients with other major collagen diseases. We additionally evaluated a possible association with disease severity. Eosinophil counts were significantly higher levels in patients with SSc than in those with other collagen diseases, whereas total leukocyte counts were not. Eosinophil counts correlated positively with both severe interstitial lung disease (ILD; r = 0.255, p = 0.033) and modified Rodnan total skin thickness score (m-Rodnan TSS) in SSc (r = 0.347, p = 0.003), but did not correlate with ILD severity in other collagen diseases. In conclusion, peripheral eosinophil counts were higher in patients with SSc than in those with other collagen diseases and were correlated with increased disease severity. Our data suggest that eosinophilic inflammation is involved in the pathogenesis and progression of SSc.

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BALF-derived fibroblasts differ from biopsy-derived fibroblasts in systemic sclerosis

Cited by 23 publications

References 26 publications

Alveolar fibroblasts in acute lung injury: biological behaviour and clinical relevance

Alveolar fibroblasts in acute lung injury: biological behaviour and clinical relevance

Fibroblast phenotypes and their activity are changed in the wound healing process after lung transplantation

Associations between peripheral blood eosinophil counts in patients with systemic sclerosis and disease severity

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