Barley anther culture

Cistué, L.; Vallés, M. P.; Echávarri, B.; Sanz, Jesús M.; Castillo, A. M.

doi:10.1007/978-94-017-1293-4_5

Cited by 42 publications

(29 citation statements)

References 3 publications

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“…Igri is a model cultivar for microspore embryogenesis, whereas Reinette and Hop are medium and low responding cultivars, respectively. Well-established standard protocols described by Cistué et al (2003) and Castillo et al (2000) were followed for evaluation of anther and isolated microspore culture response, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Twenty to forty days after anther or isolated microspore culture, embryos were subcultured on regeneration medium (Castillo et al 2000;Cistué et al 2003). Twenty plants from each zinc sulphate concentration were transferred to soil (as described by Cistué et al 2003) for further development and data analysis of seed production.…”

Section: Effect Of Znso 4 Concentration In the Culture Medium Of Isolmentioning

confidence: 99%

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Zinc sulphate improved microspore embryogenesis in barley

Echávarri

Soriano

Cistué

et al. 2008

Plant Cell Tiss Organ Cult

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Section: Methodsmentioning

confidence: 99%

Section: Effect Of Znso 4 Concentration In the Culture Medium Of Isolmentioning

confidence: 99%

Zinc sulphate improved microspore embryogenesis in barley

Echávarri

Soriano

Cistué

et al. 2008

Plant Cell Tiss Organ Cult

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“…Two hundred doubled haploid lines were derived from the F1 generation of the cross of Golden Promise × Full Pint following the anther culture protocol described by Cistué et al (12). The 200 doubled haploids, referred to as the "Oregon Promise" population, are available to the research community; instructions for accessing seed and data are available at www.barleyworld.org.…”

Section: Germplasmmentioning

confidence: 99%

“…Doubled haploid production is a process, widely used in contemporary barley breeding, that accelerates the breeding process by creating true-breeding (completely homozygous) genotypes (12). For the research described in this report, contributions to beer flavor were based on sensory assessment of nano-beers made from micromalts of the parental varieties, a sample (n = 34) of their doubled haploid progeny, and a malting variety standard (CDC Copeland) grown at three locations in Oregon, U.S.A. (Corvallis, Lebanon, and Madras).…”

mentioning

confidence: 99%

Effects of Barley (Hordeum Vulgare L.) Variety and Growing Environment on Beer Flavor

Herb

Filichkin

Fisk

et al. 2017

Journal of the American Society of Brewing Chemists

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J. Am. Soc. Brew. Chem. 75(4): [345][346][347][348][349][350][351][352][353] 2017 This research tested the hypothesis that barley genotype can affect beer flavor and assessed the relative contributions of genotype and location to beer sensory descriptors. Golden Promise, Full Pint, 34 of their doubled haploid progeny, and CDC Copeland were grown at three locations in Oregon, U.S.A. Grain from these trials was micromalted and the resulting malts used for nano-brewing. Sensory evaluations were conducted on the nano-brews. Barley genotype had significant effects on many sensory descriptors. The most significant sensory descriptorswhen comparing barley genotypes-were cereal, color, floral, fruity, grassy, honey, malty, toasted, toffee, and sweet. Golden Promise was significantly higher in fruity, floral, and grassy flavors, whereas Full Pint was significantly higher in malty, toffee, and toasted flavors. CDC Copeland was closest to neutral for most flavor traits. There were notable differences for some descriptors between locations. New combinations of parental flavor attributes were observed in the progeny. Multitrait analysis revealed regions of the barley genome with significant effects on malting quality and flavor traits. These findings are, of course, applicable only to the barley germplasm tested, the environment sampled, and the protocols used for micromalting and brewing. The necessary largerscale experiments involving optimized malts and larger volumes of beer are in process.

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“…The stress pretreatment consisted of incubating the anthers in a medium containing 0.7 M mannitol, 40 mM CaCl 2 and 8g/l agarose, for four days at 25°C in the dark (Cistué et al 2003). The recently developed Barley1 GeneChip (Close et al 2004) was used for this study, thus enabling the analysis of more than 22,000 genes simultaneously.…”

Section: Expression Profile Associated With Stress Pretreatmentmentioning

confidence: 99%

Expression Profiles in Barley Microspore Embryogenesis

Muñoz‐Amatriaín

Svensson

Castillo

et al.

Advances in Haploid Production in Higher Plants

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In barley, microarray technology has allowed to study the gene expression profiling associated with the stress pretreatment phase of microspore embryogenesis, where the reprogramming of microspores takes place. Transcriptome analysis of anthers before and after four days of mannitol pretreatment, revealed changes in the expression of 2,673 genes.A thorough study of these differentially expressed genes indicated that microspores maintained their cell cycle machinery in a steady state during stress pretreatment and underwent a major reorganization of metabolic pathways accompanied by a multidimensional stress response. Up-regulation of transcription factors related to stress responses and changes in developmental programmes took place during the pretreatment. Preliminary studies have indicated that YABBY5, ZML2, CURLY LEAF and ICE1 transcription factors have a stress pretreatment specific induction and, therefore, could play a direct role in microspore reprogramming.

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Barley anther culture

Cited by 42 publications

References 3 publications

Zinc sulphate improved microspore embryogenesis in barley

Zinc sulphate improved microspore embryogenesis in barley

Effects of Barley (Hordeum Vulgare L.) Variety and Growing Environment on Beer Flavor

Expression Profiles in Barley Microspore Embryogenesis

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