Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm

Oñate, Luis; Vicente‐Carbajosa, Jesús; Lara, Pilar; Dı́az, Isabel; Carbonero, Pilar

doi:10.1074/jbc.274.14.9175

Cited by 117 publications

(138 citation statements)

References 47 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…GLM is an essential positive element for the endosperm-specific gene expression. Oñate et al (1999) demonstrated that BLZ2, the bZIP class of transcription factors, binded specifically to the GLM motif (5 0 -GTGAGTCAT-3 0 ) in the promoter region of a B1-hordein gene. Binding by BLZ2 is prevented when the GLM mutated to 5 0 -GTGctTCtc-3 0 .…”

Section: Discussionmentioning

confidence: 99%

“…The EB box contained two distinct nuclear protein binding sites: the prolamin box (PB, 5 0 -TGTAAAG-3 0 ) also referred to as the endosperm motif (EM), and a GCN4-like motif (GLM, 5 0 -(G/A)TGA(G/C)TCA(T/C)-3 0 ). These two motifs are present in B-and C-hordein promoters (Oñate et al 1999). The Dof class of transcription factors can bind specifically to EM.…”

Section: Introductionmentioning

confidence: 99%

“…Any base mutation in AAAG would abolish the binding, and the flanking of the core sequences also had some influence on the binding. The bZIP class of transcription factors can bind specifically to GLM (Holdsworth et al 1995), and moreover, interaction between Dof and bZIP factors was also found to be required for transcription (Oñate et al 1999). So far, there are few reports about B-hordein gene promoters (Forde et al 1985b;Marris et al 1988).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Han

Deng

et al. 2009

Genetica

View full text Add to dashboard Cite

The B-hordein gene family was analyzed from two Tibetan hull-less barley cultivars Z09 and Z26 (Hordeum vulgare subsp. vulgare). Fourteen B-hordein genes, designated BZ09-2 to BZ09-5 (from Z09) and BZ26-1 to BZ26-10 (from Z26), were sequenced. Seven of them, similar to a previously reported BZ09-1 from Z09, were predicted to encode putative active proteins each with a signal peptide, a repetitive domain, and a C-terminal region; seven of them were predicted to be pseudogenes. The B-hordein gene family was analyzed using all known representatives of B-hordein sequences and two most similar LMW-GSs of Triticum aestivum. Alignment of these seven putative proteins with known B-hordeins and two most similar LMW-GSs of T. aestivum revealed that they shared a common motif. A large variation was observed between numbers of repeat units of predicted B-hordeins of Z26 and Z09. Phylogenetic analysis revealed that all BZ26 clones were clustered in a subgroup, and BZ09-1 formed another subgroup by itself in the putative eight active genes. In addition, six 5 0 -upstream regulatory sequences of the B-hordein genes were isolated from the two accessions by a single oligonucleotide nested PCR, and several different mutations were identified in the cis-acting element GLM and two distinctive sequences (Z09P-2 and Z26P-3). Phylogenetic analysis of 5 0 -upstream regulatory regions of the B-hordein genes showed that members from the same accession were clustered together except for two distinct members. Quantitative real time PCR analysis indicated distinct expression levels of B-hordein genes in four developing stages of developing grains in two accessions. These findings suggest B-hordein genes have intrinsic differences between accessions, and this knowledge will be useful for incorporating the B-hordeins protein in barley breeding programs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Han

Deng

et al. 2009

Genetica

View full text Add to dashboard Cite

show abstract

“…An understanding of gene expression control in the seed was tackled from early studies in plant molecular biology, with maize (Zea mays) Opaque2 (O2) representing a hallmark as one of the first plant transcription factor (TF) genes cloned and characterized (Hartings et al, 1989;Schmidt et al, 1990). Similarly, orthologous genes from wheat (Triticum aestivum) (SPA) and barley (Hordeum vulgare) (BLZ2) play the same roles as O2 in their corresponding species (Albani et al, 1997;Oñ ate et al, 1999). In dicot species, key TFs have been characterized that control gene expression programs during seed maturation.…”

Section: Introductionmentioning

confidence: 99%

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

et al. 2009

View full text Add to dashboard Cite

Transcription of Arabidopsis thaliana seed maturation (MAT) genes is controlled by members of several transcription factor families, such as basic leucine zippers (bZIPs), B3s, MYBs, and DOFs. In this work, we identify Arabidopsis bZIP53 as a novel transcriptional regulator of MAT genes. bZIP53 expression in developing seeds precedes and overlaps that of its target genes. Gain-and loss-of-function approaches indicate a correlation between the amount of bZIP53 protein and MAT gene expression. Specific in vivo and in vitro binding of bZIP53 protein to a G-box element in the albumin 2S2 promoter is demonstrated. Importantly, heterodimerization with bZIP10 or bZIP25, previously described bZIP regulators of MAT gene expression, significantly enhances DNA binding activity and produces a synergistic increase in target gene activation. Fulllevel target gene activation is strongly correlated with the ratio of the correspondent bZIP heterodimerization partners. Whereas bZIP53 does not interact with ABI3, a crucial transcriptional regulator in Arabidopsis seeds, ternary complex formation between the bZIP heterodimers and ABI3 increases the expression of MAT genes in planta. We therefore propose that heterodimers containing bZIP53 participate in enhanceosome formation to produce a dramatic increase in MAT gene transcription.

show abstract

“…In vitro DNase I footprinting with nuclear proteins from rice maturing seeds and GST-O2 fusion protein also showed that the sequence covering the GCN4 motif in the promoter of glutelin genes is specifically protected from DNase I digestion (8,17). These findings suggest that O2-like transcription factor may exist and participate in controlling the endosperm-specific expression of many storage protein genes in cereal seed through the GCN4 motif.The cDNA clones encoding transcription factor recognizing the GCN4 motif have been recently isolated and characterized in wheat (5) and barley (18,19). These transcription factors, designated SPA, BLZ1, and BLZ2, transcriptionally activate expression of the storage protein genes by interacting with the GCN4 motif in the wheat low molecular weight glutenin and barley B1-hordein promoters.…”

mentioning

confidence: 99%

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Onodera¹,

Suzuki²,

Wu³

et al. 2001

Journal of Biological Chemistry

152

149

View full text Add to dashboard Cite

The GCN4 motif, a cis-element that is highly conserved in the promoters of cereal seed storage protein genes, plays a central role in controlling endospermspecific expression. This motif is the recognition site for a basic leucine zipper transcriptional factor that belongs to the group of maize Opaque-2 (O2)-like proteins. Five different basic leucine zipper cDNA clones, designated RISBZ1-5, have been isolated from a rice seed cDNA library. The predicted gene products can be divided into two groups based on their amino acid sequences. Although all the RISBZ proteins are able to interact with the GCN4 motif, only RISBZ1 is capable of activating (more than 100-fold expression) the expression of a reporter gene under a minimal promoter fused to a pentamer of the GCN4 motif. Loss-of-function and gain-of-function experiments using the yeast GAL4 DNA binding domain revealed that the proline-rich N-terminal domain (27 amino acids in length) is responsible for transactivation. The RISBZ1 protein is capable of forming homodimers as well as heterodimers with other RISBZ subunit proteins. RISBZ1 gene expression is restricted to the seed, where it precedes the expression of storage protein genes. When the RISBZ1 promoter was transcriptionally fused to the ␤-glucuronidase reporter gene and the chimeric gene was introduced into rice, the ␤-glucuronidase gene is specifically expressed in aleurone and subaleurone layer of the developing endosperm. These findings suggest that the specific expression of transcriptional activator RISBZ1 gene may determine the endosperm specificity of the storage protein genes.Regulated gene expression is mediated by the combinatorial interactions of multiple cis-elements in the gene's promoter. Specific binding of transcriptional factors to the cognate ciselements constitute a crucial step in transcription initiation and, in turn, on the spatial and temporal expression of genes.Seed storage protein genes provide a model system for the study on the regulatory mechanisms of plant genes (1), since their expression is restricted to a specific tissue and stage during seed development. These specific temporal and spatial expression patterns may be explained as the result of regulatory assemblies of several transcriptional activators that recognize the cis-elements implicated in seed-specific expression. Therefore, to understand such molecular mechanisms, characterization of cis-elements and transcription factors has been performed on many storage protein genes of several crop plants (2,3). Despite numerous studies, the mechanism by which these genes are regulated are poorly understood, since many of the essential cis-elements have not been identified. This is especially true in the case of monocot plants, where many of the promoter analyses of cereal storage protein genes have carried out by transient assays using particle bombardment or heterologous transgenic tobacco system (4 -6). Dissection analyses of promoter using homologous stable transgenic plant have been carried out only on glutelin genes of ...

show abstract

Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm

Cited by 117 publications

References 47 publications

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation ofArabidopsisSeed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Contact Info

Product

Resources

About