Bartonellosis: A One Health Perspective

Pultorak, Elizabeth L.; Maggi, Ricardo G.; Breitschwerdt, Edward B.

doi:10.1007/978-4-431-55120-1_6

Cited by 4 publications

(3 citation statements)

References 216 publications

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Section: Introductionmentioning

confidence: 99%

“…The genus Bartonella is a group of Gram-negative, fastidious, facultative intraerythrocytic bacteria in the family Bartonellaceae [ 1 ]. Species of Bartonella are regarded as emerging zoonotic pathogens, transmitted from vertebrate animal hosts to humans via arthropod vectors such as fleas, biting flies, and lice [ 1 ]. Twenty-five recognized species of Bartonella have been isolated from wild small mammal hosts such as rodents and shrews, highlighting the involvement of these animals in sylvatic Bartonella cycles [ 1 – 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Species of Bartonella are regarded as emerging zoonotic pathogens, transmitted from vertebrate animal hosts to humans via arthropod vectors such as fleas, biting flies, and lice [ 1 ]. Twenty-five recognized species of Bartonella have been isolated from wild small mammal hosts such as rodents and shrews, highlighting the involvement of these animals in sylvatic Bartonella cycles [ 1 – 3 ]. There is an incomplete understanding of Bartonella reservoir ecology, and novel Bartonella species continue to be detected in different wildlife hosts and new geographic areas, emphasizing the need for continued surveillance [ 1 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies

Jeeves,

Fernando,

Kotwa

et al. 2024

BMC Res Notes

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Objective Bartonella are emerging bacterial zoonotic pathogens. Utilization of clotted blood samples for surveillance of these bacteria in wildlife has begun to supersede the use of tissues; however, the efficacy of these samples has not been fully investigated. Our objective was to compare the efficacy of spleen and blood samples for DNA extraction and direct detection of Bartonella spp. via qPCR. In addition, we present a protocol for improved DNA extraction from clotted, pelleted (i.e., centrifuged) blood samples obtained from wild small mammals. Results DNA concentrations from kit-extracted blood clot samples were low and A260/A280 absorbance ratios indicated high impurity. Kit-based DNA extraction of spleen samples was efficient and produced ample DNA concentrations of good quality. We developed an in-house extraction method for the blood clots which resulted in apposite DNA quality when compared to spleen samples extracted via MagMAX DNA Ultra 2.0 kit. We detected Bartonella in 9/30 (30.0%) kit-extracted spleen DNA samples and 11/30 (36.7%) in-house-extracted blood clot samples using PCR. Our results suggest that kit-based methods may be less suitable for DNA extraction from blood clots, and that blood clot samples may be superior to tissues for Bartonella detection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies

Jeeves,

Fernando,

Kotwa

et al. 2024

BMC Res Notes

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Cattle Farming and Plantation Forest are Associated with Bartonella Occurrence in Wild Rodents

Colombo,

Antoniazzi,

Cicuttin

et al. 2023

EcoHealth

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Molecular pathogen screening of louse flies (Diptera: Hippoboscidae) from domestic and wild ruminants in Austria

Peña-Espinoza,

Em,

Shahi-Barogh

et al. 2023

Parasites Vectors

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Background Hippoboscid flies (Diptera: Hippoboscidae), also known as louse flies or keds, are obligate blood-sucking ectoparasites of animals, and accidentally of humans. The potential role of hippoboscids as vectors of human and veterinary pathogens is being increasingly investigated, but the presence and distribution of infectious agents in louse flies is still unknown in parts of Europe. Here, we report the use of molecular genetics to detect and characterize vector-borne pathogens in hippoboscid flies infesting domestic and wild animals in Austria. Methods Louse flies were collected from naturally infested cattle (n = 25), sheep (n = 3), and red deer (n = 12) across Austria between 2015 and 2019. Individual insects were morphologically identified to species level and subjected to DNA extraction for molecular pathogen screening and barcoding. Genomic DNA from each louse fly was screened for Borrelia spp., Bartonella spp., Trypanosomatida, Anaplasmataceae, Filarioidea and Piroplasmida. Obtained sequences of Trypanosomatida and Bartonella spp. were further characterized by phylogenetic and haplotype networking analyses. Results A total of 282 hippoboscid flies corresponding to three species were identified: Hippobosca equina (n = 62) collected from cattle, Melophagus ovinus (n = 100) from sheep and Lipoptena cervi (n = 120) from red deer (Cervus elaphus). Molecular screening revealed pathogen DNA in 54.3% of hippoboscids, including infections with single (63.39%), two (30.71%) and up to three (5.90%) distinct pathogens in the same individual. Bartonella DNA was detected in 36.9% of the louse flies. Lipoptena cervi were infected with 10 distinct and previously unreported Bartonella sp. haplotypes, some closely associated with strains of zoonotic potential. DNA of trypanosomatids was identified in 34% of hippoboscids, including the first description of Trypanosoma sp. in H. equina. Anaplasmataceae DNA (Wolbachia spp.) was detected only in M. ovinus (16%), while < 1% of the louse flies were positive for Borrelia spp. and Filarioidea. All hippoboscids were negative for Piroplasmida. Conclusions Molecular genetic screening confirmed the presence of several pathogens in hippoboscids infesting domestic and wild ruminants in Austria, including novel pathogen haplotypes of zoonotic potential (e.g. Bartonella spp.) and the first report of Trypanosoma sp. in H. equina, suggesting a potential role of this louse fly as vector of animal trypanosomatids. Experimental transmission studies and expanded monitoring of hippoboscid flies and hippoboscid-associated pathogens are warranted to clarify the competence of these ectoparasites as vectors of infectious agents in a One-Health context. Graphical Abstract

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Bartonellosis: A One Health Perspective

Cited by 4 publications

References 216 publications

Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies

Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies

Cattle Farming and Plantation Forest are Associated with Bartonella Occurrence in Wild Rodents

Molecular pathogen screening of louse flies (Diptera: Hippoboscidae) from domestic and wild ruminants in Austria

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