Based on Network Pharmacology and Gut Microbiota Analysis to Investigate the Mechanism of the Laxative Effect of Pterostilbene on Loperamide-Induced Slow Transit Constipation in Mice

Yao, Zhiwei; Fu, Siqi; Ren, Beibei; Ma, Lushun; Sun, Daqing

doi:10.3389/fphar.2022.913420

Cited by 23 publications

(15 citation statements)

References 47 publications

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“…Gut microbiota are closely related to host health and influenced by nutritional intake and lifestyle . Previous studies have shown that prebiotics can reduce constipation by regulating the microbial composition of the intestinal tract. , Both polyphenols and polysaccharides extracted from FBT have been proven to regulate intestinal flora and maintain host health. , FTE is extracted from FBT infusion, which has been proven to promote the colonization of beneficial bacteria such as Roseburia and Lactobacillus . Here, the 16S rRNA gene amplicon sequencing technology was used to analyze gut microbial changes.…”

Section: Discussionmentioning

confidence: 99%

“…52 Previous studies have shown that prebiotics can reduce constipation by regulating the microbial composition of the intestinal tract. 53,54 Both polyphenols and polysaccharides extracted from FBT have been proven to regulate intestinal flora and maintain host health. 25,26 FTE is extracted from FBT infusion, which has been proven to promote the colonization of beneficial bacteria such as Roseburia and Lactobacillus.…”

Section: ■ Discussionmentioning

confidence: 99%

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Fu Brick Tea Alleviates Constipation via Regulating the Aquaporins-Mediated Water Transport System in Association with Gut Microbiota

Zhang

et al. 2023

J. Agric. Food Chem.

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This study aimed to investigate the amendatory effects of Fu brick tea aqueous extract (FTE) on constipation and its underlying molecular mechanism. The administration of FTE by oral gavage (100 and 400 mg/kg•bw) for 5 weeks significantly increased fecal water content, improved difficult defecation, and enhanced intestinal propulsion in loperamide (LOP)-induced constipated mice. FTE also reduced colonic inflammatory factors, maintained the intestinal tight junction structure, and inhibited colonic Aquaporins (AQPs) expression, thus normalizing the intestinal barrier and colonic water transport system of constipated mice. 16S rRNA gene sequence analysis results indicated that two doses of FTE increased the Firmicutes/Bacteroidota (F/B) ratio at the phylum level and increased the relative abundance of Lactobacillus from 5.6 ± 1.3 to 21.5 ± 3.4% and 28.5 ± 4.3% at the genus level, subsequently resulting in a significant elevation of colonic contents short-chain fatty acids levels. The metabolomic analysis demonstrated that FTE improved levels of 25 metabolites associated with constipation. These findings suggest that Fu brick tea has the potential to alleviate constipation by regulating gut microbiota and its metabolites, thereby improving the intestinal barrier and AQPs-mediated water transport system in mice.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Fu Brick Tea Alleviates Constipation via Regulating the Aquaporins-Mediated Water Transport System in Association with Gut Microbiota

Zhang

et al. 2023

J. Agric. Food Chem.

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“…63 PT can also improve intestinal inflammation and motility disorders, promoting the richness and diversity of probiotics. 64 Our past research has shown that PT effectively increases the relative abundance of Bacteroidetes and decreases the relative abundance of Firmicutes. 65 Recently, PT has been reported to reduce inflammation and alter the gut microbiota in rheumatoid arthritis.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Polyphenol compounds such as resveratrol and PT are recommended as dietary additions due to their antiobesity effect . PT can also improve intestinal inflammation and motility disorders, promoting the richness and diversity of probiotics . Our past research has shown that PT effectively increases the relative abundance of Bacteroidetes and decreases the relative abundance of Firmicutes .…”

Section: Discussionmentioning

confidence: 99%

Pterostilbene Protects against Osteoarthritis through NLRP3 Inflammasome Inactivation and Improves Gut Microbiota as Evidenced by In Vivo and In Vitro Studies

Lee,

Chang,

Cheng

et al. 2024

J. Agric. Food Chem.

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Osteoarthritis (OA) is a persistent inflammatory disease, and long-term clinical treatment often leads to side effects. In this study, we evaluated pterostilbene (PT), a natural anti-inflammatory substance, for its protective effects and safety during prolonged use on OA. Results showed that PT alleviated the loss of chondrocytes and widened the narrow joint space in an octacalcium phosphate (OCP)-induced OA mouse model (n = 3). In vitro experiments demonstrate that PT reduced NLRP3 inflammation activation (relative protein expression: C: 1 ± 0.09, lipopolysaccharide (LPS): 1.14 ± 0.07, PT: 0.91 ± 0.07, LPS + PT: 0.68 ± 0.04) and the release of inflammatory cytokines through NF-κB signaling inactivation (relative protein expression: C: 1 ± 0.03, LPS: 3.49 ± 0.02, PT: 0.66 ± 0.08, LPS + PT: 2.78 ± 0.05), ultimately preventing cartilage catabolism. Interestingly, PT also altered gut microbiota by reducing inflammation-associated flora and increasing the abundance of healthy bacteria in OA groups. Collectively, these results suggest that the PT can be considered as a protective strategy for OA.

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“…In the past decades, as a neurodegenerative disease, the incidence of AD has increased year by year, threatening more and more elderly population ( Li et al, 2021 ; Yao et al, 2022 ). As a natural medicine, AOF contains a variety of components and has the advantage of multiple targets, which also brings great challenges to researchers ( Zuo et al, 2021 ; Peng et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Alpiniae oxyphyllae fructus possesses neuroprotective effects on H2O2 stimulated PC12 cells via regulation of the PI3K/Akt signaling Pathway

Wang²,

Zhang³

et al. 2022

Front. Pharmacol.

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Backgroud: Alzheimer’s disease (AD) is a typical neurodegenerative disease, which occurs in the elderly population. Alpiniae oxyphyllae Fructus (AOF) is a traditional Chinese medicine that has potential therapeutic effect on AD, but the mechanism behind it is unclear.Methods: Firstly, the main chemical components of AOF were identified by LC-MS, while the main active ingredients and targets were screened by TCMSP database. At the same time, AD-related target proteins were obtained using Genecards and OMIM databases. PPI was constructed by cross-linking AOF and AD targets, and GO enrichment analysis and KEGG pathway enrichment analysis were performed to identify the relevant biological processes and signaling pathways. Finally, based on the H2O2-stimulated PC12 cell, flow cytometry, WB and immunofluorescence experiments were performed to verify the protective effect of AOF on AD.Results: We identified 38 active ingredients with 662 non-repetitive targets in AOF, of which 49 were potential therapeutic AD targets of AOF. According to the GO and KEGG analysis, these potential targets are mainly related to oxidative stress and apoptosis. The role of AOF in the treatment of AD is mainly related to the PI3K/AKT signaling pathway. Protocatechuic acid and nootkatone might be the main active ingredients of AOF. In subsequent experiments, the results of CCK-8 showed that AOF mitigated PC12 cell damage induced by H2O2. Kits, flow cytometry, and laser confocal microscopy indicated that AOF could decrease ROS and increase the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), while AOF could also increase mitochondrial membrane potential (MMP), thereby inhibiting apoptosis. Finally, immunofluorescence and WB results showed that AOF inhibited the expression of BAX and caspase-3 in PC12 cells, and promoted the expression of Bcl-2. At the same time, the phosphorylation levels of PI3K and Akt proteins were also significantly increased.Conclusion: This study suggests that AOF had the potential to treat AD by suppressing apoptosis induced by oxidative stress via the PI3K/Akt pathway.

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Based on Network Pharmacology and Gut Microbiota Analysis to Investigate the Mechanism of the Laxative Effect of Pterostilbene on Loperamide-Induced Slow Transit Constipation in Mice

Cited by 23 publications

References 47 publications

Fu Brick Tea Alleviates Constipation via Regulating the Aquaporins-Mediated Water Transport System in Association with Gut Microbiota

Fu Brick Tea Alleviates Constipation via Regulating the Aquaporins-Mediated Water Transport System in Association with Gut Microbiota

Pterostilbene Protects against Osteoarthritis through NLRP3 Inflammasome Inactivation and Improves Gut Microbiota as Evidenced by In Vivo and In Vitro Studies

Alpiniae oxyphyllae fructus possesses neuroprotective effects on H2O2 stimulated PC12 cells via regulation of the PI3K/Akt signaling Pathway

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