Basement Membrane Assembly and Differentiation of Cultured Corneal Cells: Importance of Culture Environment and Endothelial Cell Interaction

Zieske, James D.; Mason, Valerie S.; Wasson, Michael E.; Meunier, Susan F.; Nolte, Cynthia J. M.; Fukai, Naomi; Olsen, Bjørn R.; Parenteau, Nancy L.

doi:10.1006/excr.1994.1300

Cited by 169 publications

(106 citation statements)

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“…Similarly, epithelial cells seeded on a collagen sponge and co-cultured with or separated from endothelial cells by a transwell culture insert formed three to four layers of epithelial cells by the second week in culture, compared with the single layer of epithelial cells observed when cultured on collagen alone (30). Zieske et al co-cultured all three corneal cell types together in a threedimensional tissue construct to investigate the effect of culture conditions and endothelial cell interaction on epithelial cell differentiation and basement membrane assembly (31). A basal layer of endothelial cells was covered with a middle layer of stromal fibroblasts in a collagen gel matrix and covered with a top layer of epithelial cells.…”

Section: Cell-based Approaches To a Tissue-engineered Corneamentioning

confidence: 99%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

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ABSTRACT:The field of corneal tissue engineering has made many strides in recent years. The challenges of engineering a biocompatible, mechanically stable, and optically transparent tissue are significant. To overcome these challenges, researchers have adopted two basic approaches: cell-based strategies for manipulating cells to create their own extracellular matrix, and scaffold-based strategies for providing strong and transparent matrices upon which to grow cells. Both strategies have met with some degree of success. In addition, recent advances have been made in innervating a tissueengineered construct. Future work will need to focus on further improving mechanical stability of engineered constructs as well as improving the host response to implantation.

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Section: Cell-based Approaches To a Tissue-engineered Corneamentioning

confidence: 99%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

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“…A variety of connective tissue substrates have been used to construct these HSEs to generate stratified epithelia that display many morphologic and functional characteristics of native skin. However, while HSEs express BM components (Fusenig 1994;Marinkovich et al 1993), only limited success has been realized in demonstrating morphologically-identifiable BM in these cultures (Zieske et al 1994, Andriani et al 2003. Since intact BM is known to be a critical signal for the control of normal epidermal growth and differentiation, it is important to fabricate tissues that generate structured BM.…”

Section: Introductionmentioning

confidence: 99%

The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Segal¹,

Andriani²,

Pfeiffer³

et al. 2008

Matrix Biology

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Epithelial-mesenchymal interactions promote the morphogenesis and homeostasis of human skin. However, the role of the basement membrane (BM) during this process is not well-understood. To directly study how BM proteins influence epidermal differentiation, survival and growth, we developed novel 3D human skin equivalents (HSEs). These tissues were generated by growing keratinocytes at an air-liquid interface on polycarbonate membranes coated with individual matrix proteins (Type I Collagen, Type IV Collagen or fibronectin) that were placed on contracted Type I Collagen gels populated with dermal fibroblasts. We found that only keratinocytes grown on membranes coated with the BM protein Type IV Collagen showed optimal tissue architecture that was similar to control tissues grown on de-epidermalized dermis (AlloDerm) that contained intact BM. In contrast, tissues grown on proteins not found in BM, such as fibronectin and Type I Collagen, demonstrated aberrant tissue architecture that was linked to a significant elevation in apoptosis and lower levels of proliferation of basal keratinocytes. While all tissues demonstrated a normalized, linear pattern of deposition of laminin 5, tissues grown on Type IV Collagen showed elevated expression of α6 integrin, Type IV Collagen and Type VII Collagen, suggesting induction of BM organization. Keratinocyte differentiation (Keratin 1 and filaggrin) was not dependent on the presence of BM proteins. Thus, Type IV Collagen acts as a critical microenvironmental factor in the BM that is needed to sustain keratinocyte growth and survival and to optimize epithelial architecture.

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“…Extracellular matrix (ECM) and BM components acting through cell surface adhesive receptors, integrins (Giancotti 1997), play an important role in the adhesion, migration, and differentiation of various cells (Azar et al 1992;Kurpakus et al 1992;Zieske et al 1994). We therefore hypothesized that corneal epithelial abnormalities in diabetes may be due to altered BM structure and/or epithelial integrin expression.…”

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confidence: 99%

Human Corneal Epithelial Basement Membrane and Integrin Alterations in Diabetes and Diabetic Retinopathy¹

Ljubimov

Huang

et al. 1998

J Histochem Cytochem.

103

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SUMMARYCorneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix (ECM) and basement membrane (BM). To identify such alterations, various ECM and BM components and integrin receptors were studied by immunofluorescence on sections of normal and diabetic human corneas. Age-matched corneas from 15 normal subjects, 10 diabetics without diabetic retinopathy (DR), and 12 diabetics with DR were used. In DR corneas, the composition of the central epithelial BM was markedly altered, compared to normal or non-DR diabetic corneas. In most cases the staining for entactin/nidogen and for chains of laminin-1 ( ␣1 ␤1 ␥1) and laminin-10 ( ␣5 ␤1 ␥1) was very weak, discontinuous, or absent over large areas. Other BM components displayed less frequent changes. The staining for ␣3 ␤1 (VLA-3) laminin binding integrin was also weak and discontinuous in DR corneal epithelium. Components of stromal ECM remained unchanged even in DR corneas. Therefore, distinct changes were identified in the composition of the epithelial BM in DR corneas. They may be due to increased degradation or decreased synthesis of BM components and related integrins. These alterations may directly contribute to the epithelial adhesion and wound healing abnormalities found in diabetic corneas. (J Histochem Cytochem 46:1033-1041, 1998)

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Basement Membrane Assembly and Differentiation of Cultured Corneal Cells: Importance of Culture Environment and Endothelial Cell Interaction

Cited by 169 publications

References 0 publications

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Human Corneal Epithelial Basement Membrane and Integrin Alterations in Diabetes and Diabetic Retinopathy¹

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Basement Membrane Assembly and Differentiation of Cultured Corneal Cells: Importance of Culture Environment and Endothelial Cell Interaction

Cited by 169 publications

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The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Human Corneal Epithelial Basement Membrane and Integrin Alterations in Diabetes and Diabetic Retinopathy1

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Human Corneal Epithelial Basement Membrane and Integrin Alterations in Diabetes and Diabetic Retinopathy¹