Basic fibroblast growth factor promotes the trans-differentiation of mouse bone marrow cells into hepatic lineage cells via multiple liver-enriched transcription factors

Saji, Yukiko; Tamura, Shinji; Yoshida, Yasuhiko; Kiso, Shinichi; Iizuka, Ayuko; Matsumoto, Hitoshi; Kawasaki, Tatsuya; Kamada, Y.; Matsuzawa, Yūji; Shinomura, Yasuhisa

doi:10.1016/j.jhep.2004.06.017

Cited by 26 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To our knowledge, one study examined the differentiation of HNF3b-transfected embryonic stem cells (ESC) in spheroidal aggregates [19], but no one has reported the hepatic differentiation of bone marrow MSC in a cell pellet configuration. Similarly, some studies induced hepatic differentiation of bone marrow stem cells and ESC in 3D collagen type I gels and scaffolds [20,21], but hepatic differentiation of bone marrow MSC on the SIS biomaterial has not been studied. Fig.…”

Section: Discussionmentioning

confidence: 99%

Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture

2006

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture

2006

View full text Add to dashboard Cite

“…hepatocyte growth factors (HGF), fibroblast growth factor (FGF) and oncostatin M] and culture layers specific for hepatocyte growth, like matrigel. To reproduce the pathophysiological conditions of liver injury, some studies also employed cholestatic serum or co-culture with chemically damaged liver tissue [12][13][14] . Although these studies showed some HSC "transdifferentiation" into hepatocytes, the reported percentage of hepatocytes derived from HSCs did not exceed 5%.…”

Section: Bone Marrow-derived Stem Cellsmentioning

confidence: 99%

Stem cells for end stage liver disease: How far have we got?

Lorenzini¹,

Gitto²,

Grandini³

et al. 2008

WJG

View full text Add to dashboard Cite

End stage liver disease (ESLD) is a health problem worldwide. Liver transplantation is currently the only effective therapy, but its many drawbacks include a shortage of donors, operative damage, risk of rejection and in some cases recidivism of the pre-transplant disease. These factors account for the recent growing interest in regenerative medicine. Experiments have sought to identify an optimal source of stem cells, sufficient to generate large amounts of hepatocytes to be used in bioartificial livers or injected in vivo to repair the diseased organ. This update aims to give non-stem cell specialists an overview of the results obtained to date in this fascinating field of biomedical research.

show abstract

“…FGF2 is produced primarily in liver sinusoidal cells, such as Ito cells, and promotes the production of ECM and vascularization, thus assisting in the repair of liver injury (Hioki et al 1996). It induces DNA synthesis in primary cultured fetal and adult rat hepatocytes (Hoffmann and Paul 1990) and has also been shown to promote the transdifferentiation of mouse BMCs into hepatic lineage cells, in association with the induction of transcription factors, including hepatocyte nuclear factors and GATA family proteins (Saji et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its potential role in the differentiation of hepatic epithelial stem-like cells into the hepatocyte lineage

et al. 2010

View full text Add to dashboard Cite

The transplantation of bone marrow cells (BMCs) has been applied in liver regenerative cell therapy. However, details of the interaction between the transplanted BMCs and hepatic stem cells have not been elucidated. The aim of the present study was to investigate the interaction of BMCs with hepatic stem-like cells (HSLCs) and to determine the BMC factor that steers HSLC differentiation into the hepatocyte lineage. Both BMCs and HSLCs were obtained from an adult Sprague-Dawley rat, and a co-culture system was established. Cell proliferation was analyzed by a proliferation assay, and the differentiation of HSLCs into the hepatocyte lineage was evaluated by the detection of cellular mRNA for liver-specific proteins. DNA microarray analysis was applied to BMCs co-cultured with HSLCs to determine the genes upregulated by their interaction. The proliferation of HSLCs co-cultured with BMCs was significantly higher than that of HSLCs cultured alone, and the expression of mRNAs for both albumin and tryptophan-2,3-dioxygenase was detectable in the co-cultured HSLCs. DNA microarray analysis showed the upregulated expression of fibroblast growth factor 2 (FGF2) mRNA in BMCs co-cultured with HSLCs, and the expression of mRNAs for both albumin and tyrosine aminotransferase became detectable in HSLCs cultured with FGF2. Thus, BMCs stimulate both the proliferation of HSLCs and their differentiation into the hepatocyte lineage. FGF2 is one of the factors that is produced by the interacting BMCs and that stimulates this differentiation.

show abstract

Basic fibroblast growth factor promotes the trans-differentiation of mouse bone marrow cells into hepatic lineage cells via multiple liver-enriched transcription factors

Cited by 26 publications

References 35 publications

Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture

Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture

Stem cells for end stage liver disease: How far have we got?

Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its potential role in the differentiation of hepatic epithelial stem-like cells into the hepatocyte lineage

Contact Info

Product

Resources

About