Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

“…Histology is considered one of the most useful and accurate quality control in peripheral nerve repair/regeneration studies ( Carriel et al, 2014 ; Chato-Astrain et al, 2020a ). In this study, histological analyses confirmed a consistent and highly active nerve tissue regeneration process in the central portion of implanted grafts after 15 weeks.…”

“…Regarding the histological pattern, the nerve regeneration process was more abundant and homogeneous in animals from AUTO and P2 groups whereas an evident microfasciculation (newly-formed nerve fascicles) and signs of fibrotic response characterized the nerve regeneration process in SD and to a lesser degree P1 group, being these findings in accordance with previous studies ( Chato-Astrain et al, 2020b ). Moreover, histomorphometrical analyses, another key quality controls in the field, provided objective and quantitative values of the nerve regeneration process following peripheral nerve repair ( Raimondo et al, 2009 ; Chato-Astrain et al, 2020a ). These analyses confirmed the microfasciculation phenomena typical of regenerating nerves with smaller myelinated fibers and thinner myelin sheaths than healthy nerves ( Geuna et al, 2009 ; Raimondo et al, 2009 ; Ronchi et al, 2015 ; Lovati et al, 2018 ).…”

“…Harvested healthy and operated nerves ( n = 6) were processed for conventional histology, as described above, whereas the distal nerve stump was used for quantitative ultrastructural analysis. For conventional histology, central portion of the implanted grafts were selected and stained with histochemical and immunohistochemical methods as recommended ( Chato-Astrain et al, 2020a ; Garcia-Garcia et al, 2023 ). General morphology was evaluated by HE, and the degree of myelination and collagen organization pattern were assessed by MCOLL.…”