Basic Physicochemical Properties of Polyethylene Glycol Coated Gold Nanoparticles that Determine Their Interaction with Cells

Pino, Pablo del; Yang, Fang; Pelaz, Beatriz; Zhang, Qian; Kantner, Karsten; Hartmann, Raimo; Baroja, Natalia Martínez de; Gallego, Marta; Möller, Marco; Manshian, Bella B.; Soenen, Stefaan J.; Riedel, R.; Hampp, Norbert; Parak, Wolfgang J.

doi:10.1002/anie.201511733

Cited by 117 publications

(100 citation statements)

References 18 publications

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“…For a more detailed and comprehensive evaluation of the presented method, the effects of three different types of Au NPs on cells were investigated (details can be found in “Methods”): (i) Au(5)-PMA, i.e., Au NPs having a core diameter of 5 nm which are grafted with poly(isobutylene-alt-maleic anhydride) dodecylamine (in the following referred to as PMA); (ii) Au(13)-PMA, i.e., Au NPs having a core diameter of 13 nm coated with PMA [46]; (iii) Au(13)-PEG, i.e., Au NPs having a core diameter of 13 nm coated with polyethylene glycol (PEG) [47]. Unless otherwise specified a concentration range from 3 to 400 nM (in terms of NP concentration) was tested.…”

Section: Resultsmentioning

confidence: 99%

Real-time, label-free monitoring of cell viability based on cell adhesion measurements with an atomic force microscope

et al. 2017

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BackgroundThe adhesion of cells to an oscillating cantilever sensitively influences the oscillation amplitude at a given frequency. Even early stages of cytotoxicity cause a change in the viscosity of the cell membrane and morphology, both affecting their adhesion to the cantilever. We present a generally applicable method for real-time, label free monitoring and fast-screening technique to assess early stages of cytotoxicity recorded in terms of loss of cell adhesion.ResultsWe present data taken from gold nanoparticles of different sizes and surface coatings as well as some reference substances like ethanol, cadmium chloride, and staurosporine. Measurements were recorded with two different cell lines, HeLa and MCF7 cells. The results obtained from gold nanoparticles confirm earlier findings and attest the easiness and effectiveness of the method.ConclusionsThe reported method allows to easily adapt virtually every AFM to screen and assess toxicity of compounds in terms of cell adhesion with little modifications as long as a flow cell is available. The sensitivity of the method is good enough indicating that even single cell analysis seems possible.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0256-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Real-time, label-free monitoring of cell viability based on cell adhesion measurements with an atomic force microscope

et al. 2017

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“…The protein cages were characterized using DLS and TEM. Zeta‐potentials of the cages in assembly buffer were measured by Zetasizer Nano (Malvern Instruments Ltd.) …”

Section: Methodsmentioning

confidence: 99%

Intranasal Nanovaccine Confers Homo‐ and Hetero‐Subtypic Influenza Protection

Zhang

Sun

et al. 2018

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Cross-protective and non-invasively administered vaccines are attractive and highly desired for the control of influenza. Self-assembling nanotechnology provides an opportunity for the development of vaccines with superior performance. In this study, an intranasal nanovaccine is developed targeting the conserved ectodomain of influenza matrix protein 2(M2e). 3-sequential repeats of M2e (3M2e) is presented on the self-assembling recombinant human heavy chain ferritin (rHF) cage to form the 3M2e-rHF nanoparticle. Intranasal vaccination with 3M2e-rHF nanoparticles in the absence of an adjuvant induces robust immune responses, including high titers of sera M2e-specific IgG antibodies, T-cell immune responses, and mucosal secretory-IgA antibodies in mice. The 3M2e-rHF nanoparticles also confer complete protection against a lethal infection of homo-subtypic H1N1 and hetero-subtypic H9N2 virus. An analysis of the mechanism of protection underlying the intranasal immunization with the 3M2e-rHF nanoparticle indicates that M2e-specific mucosal secretory-IgA and T-cell immune responses may play critical roles in the prevention of infection. The results suggest that the 3M2e-rHF nanoparticle is a promising, needle-free, intranasally administered, cross-protective influenza vaccine. The use of self-assembling nanovaccines could be an ideal strategy for developing vaccines with characteristics such as high immunogenicity, cross-protection, and convenient administration, as well as being economical and suitable for large-scale production.

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“…The advent of nanoscale particles with revolutionary physico‐chemical properties, specific applicability, and predictable outcome thus accounts for the rise of nanomedicine. In the biomedical arena, the most prized nanomaterial competitors include liposomes, gold, magnetic, polymer, carbon, silicon, and mesoporous silica/organosilica nanomaterials (see Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica and Organosilica Nanoparticles: Physical Chemistry, Biosafety, Delivery Strategies, and Biomedical Applications

Croissant

Fatieiev

Almalik

et al. 2017

Adv Healthcare Materials

471

349

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Predetermining the physico-chemical properties, biosafety, and stimuli-responsiveness of nanomaterials in biological environments is essential for safe and effective biomedical applications. At the forefront of biomedical research, mesoporous silica nanoparticles and mesoporous organosilica nanoparticles are increasingly investigated to predict their biological outcome by materials design. In this review, it is first chronicled that how the nanomaterial design of pure silica, partially hybridized organosilica, and fully hybridized organosilica (periodic mesoporous organosilicas) governs not only the physico-chemical properties but also the biosafety of the nanoparticles. The impact of the hybridization on the biocompatibility, protein corona, biodistribution, biodegradability, and clearance of the silica-based particles is described. Then, the influence of the surface engineering, the framework hybridization, as well as the morphology of the particles, on the ability to load and controllably deliver drugs under internal biological stimuli (e.g., pH, redox, enzymes) and external noninvasive stimuli (e.g., light, magnetic, ultrasound) are presented. To conclude, trends in the biomedical applications of silica and organosilica nanovectors are delineated, such as unconventional bioimaging techniques, large cargo delivery, combination therapy, gaseous molecule delivery, antimicrobial protection, and Alzheimer's disease therapy.

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Basic Physicochemical Properties of Polyethylene Glycol Coated Gold Nanoparticles that Determine Their Interaction with Cells

Cited by 117 publications

References 18 publications

Real-time, label-free monitoring of cell viability based on cell adhesion measurements with an atomic force microscope

Real-time, label-free monitoring of cell viability based on cell adhesion measurements with an atomic force microscope

Intranasal Nanovaccine Confers Homo‐ and Hetero‐Subtypic Influenza Protection

Mesoporous Silica and Organosilica Nanoparticles: Physical Chemistry, Biosafety, Delivery Strategies, and Biomedical Applications

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