Basic Principles of Morphological Assessment of the State of Biological Tissues Using Laser Polarimetric Methods for Solving Forensic Medicine Problems

Bachynskіy, Viktor; Gurov, O. M.; Sarkisova, Y. V.; Ushenko, O. G.

doi:10.24061/1727-4338.xvi.1.59.2017.5

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“…Recently in forensic practice optical methods of biological tissue research using lasers have positively proved themselves, which by studying the phenomena of light scattering make it possible to obtain reliable objective data of the dynamics of pre-and post-mortem changes of the studied tissues of the human body and to diagnose with greater accuracy prescription of pathological process in biological tissues and liquids [1,2,3,4,5,11,13,14,15,16,17,18,19,20,21].…”

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confidence: 99%

Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

2020

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Differential diagnosis of the cause of death (CD) from ischemic cerebral infarction (ICI), hemorrhages of traumatic (HTG) and non-traumatic (HNG) genesis exclude the violent nature of death. The aim of our work was to develop forensic criteria for hemorrhage differentiation of traumatic and non-traumatic genesis and ICI by azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance (HBS). For the study were used native sections of HBS from 130 corpses in the case of: death from coronary heart disease – 40 of native sections (group 1 – control); HTG – 30 sections (group 2), ICI – 30 native sections (group 3), HNG – 30 native sections (group 4). Measuring the coordinate allocation meanings of parameters of polarization in the points of microscopic images was carried out at the location of the standard stokes-polarimeter. The effectiveness of intergroup differentiation of samples of deaths from traumatic hemorrhage and ischemic cerebral infarction reaches a satisfactory level and is 76-83%. Efficiency between group differentiation of samples of deaths from nontraumatic and traumatic hemorrhages reaches a satisfactory level and is 75-82%. As for differentiation between ischemic cerebral infarction and nontraumatic hemorrhages thise method is ineffective.

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