Basic principles of radial chromatography

Rachinskii, V. V.

doi:10.1016/s0021-9673(00)98643-6

Cited by 20 publications

(11 citation statements)

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“…The earliest theoretical treatment of RFC was done by Lapidus and Amundson (1950). A similar study was carried out by Rachinskii (1968). Later Inchin and Rachinskii (1973) included bulk fluid phase molecular diffusion in their modeling.…”

Section: Introductionmentioning

confidence: 85%

A theoretical study of multicomponent radial flow chromatography

Tsai

Tsao

1991

Chemical Engineering Science

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Section: Introductionmentioning

confidence: 85%

A theoretical study of multicomponent radial flow chromatography

Tsai

Tsao

1991

Chemical Engineering Science

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“…As described in a previous report, Radial Flow Chromatography (RFC) was first developed by Hopf during the 1940s. The method was adopted to separate by centrifugal forces originally, and the principle was adapted in the work of Rachinskii . The technology was improved in adsorption chromatography by Heftmann et al and the mathematical models were evaluated by Huang et al In a RFC column, the chromatographic media is held between two concentric cylindrical porous frits.…”

Section: Introductionmentioning

confidence: 99%

“…The method was adopted to separate by centrifugal forces originally, and the principle was adapted in the work of Rachinskii. 20 The technology was improved in adsorption chromatography by Heftmann et al 21 and the mathematical models were evaluated by Huang et al 22 In a RFC column, the chromatographic media is held between two concentric cylindrical porous frits. The mobile phase flows from the outer surface to the inner surface, across the radius of the column.…”

Section: Introductionmentioning

confidence: 99%

Efficient purification of antiproliferative polysaccharides from Hypsizigusmarmoreus with radial flow chromatography

Yan

Cao

Zhang

2014

Biotechnology Progress

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The increasing commercial significance of natural polysaccharides for use in medicinal products is stimulating the development of efficient and easy scale‐up techniques for polysaccharide purification. In this research, the crude polysaccharides from submerged cultivation broth of Hypsizigus marmoreus were purified using radial flow chromatography (RFC), and the antiproliferative activity of the purified fractions was evaluated in vitro. DEAE Sepharose CL‐6B was selected to be packed in the RFC column based on its good resolution, physical stability, and low cost. Compared with axial flow chromatography (AFC), an efficient chromatographic process with significantly less time and buffer consumption but yielding higher polysaccharide recovery and resolution was established in RFC, which could clearly purify the crude polysaccharides into different fractions. An acceptable linear scale‐up effect of RFC from 100 to 500 mL was successfully achieved without loss of resolution and enhancement of time consumption. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assays in cell cultures indicated that the purified polysaccharide fractions possess moderate antiproliferative activities in three different human cancer cell lines, but have significantly lower cytotoxicity in normal human cell lines in vitro. Among the polysaccharide fractions, the main purified acidic fraction W‐I could be considered as a novel potential antitumor agent candidate for several tumors, especially for human alveolar epithelial tumors. This research confirmed for the first time that RFC would be a new fast and efficient tool for purification of polysaccharides into different fractions, both at laboratory and commercial scales. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:872–878, 2014

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“…While several workers have developed models for radial flow chromatography [2,3], this work represents the first model to simulate ion exchange performance in a larger scale radial bed.…”

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Comparison of Simulated Performance of Fixed Ion Exchange Beds in Linear and Radial Flow

Tsaur¹,

Shallcross²

1997

Solvent Extraction and Ion Exchange

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Simulations are performed using models developed to predict ion exchange performance in fixed cylindrical resin beds. One model predicts performance in a conventional bed in which the flow is parallel to the bed's axial, while the other model predicts performance in a wedge-shaped radial bed. In this radial bed the injected solution flows radially from the inlet located at the annular bed's axis to the outlet located at the bed's periphery. The simulated breakthrough curves for the exhaustion and regeneration cycles for the Ca-H cation exchange system are compared to study the effects of operating conditions. It is found that because dispersion grows less in radial flow than in linear flow, radial ion exchange processes are more efficient than the conventional ion exchange column processes under identical operating conditions for the geometries simulated.

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Basic principles of radial chromatography

Cited by 20 publications

References 0 publications

A theoretical study of multicomponent radial flow chromatography

A theoretical study of multicomponent radial flow chromatography

Efficient purification of antiproliferative polysaccharides from Hypsizigusmarmoreus with radial flow chromatography

Comparison of Simulated Performance of Fixed Ion Exchange Beds in Linear and Radial Flow

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