Basic responses of mesenchymal stem cells exposed to bovine biomaterial and platelet rich fibrin

Abstract

The scaffolds and their interaction with mesenchymal stem cells are objects of study in bioengineering and tissue repair. Mechanisms such as surface adhesion, proliferation, viability, and cytotoxicity are essential for the development of therapies. The present study analyzed the influence of platelet-rich fibrin (PRF) in viability, cytotoxicity, and proliferation of stem cells from human exfoliated deciduous teeth (SHED) exposed to bovine biomaterial surfaces. The studied groups were divided and analyzed as follows: (S) only SHED as control Group; (SB) SHED + biomaterial; (SBP) SHED + biomaterial + PRF. Analyses of cells seeded in 24-well plates were performed after 24, 48 and 72 hours. Individual groups were subjected to viability, cytotoxicity and cell proliferation tests using neutral red, MTT and crystal violet, respectively; and in the 72-hour group, scanning electron microscopy (SEM) was performed to record cell ultra-morphology. Data were submitted to statistical analysis by two-factor ANOVA with a significance level of 5%. The results demonstrated a better performance in the viability/cytotoxicity and proliferation of stem cells for the group (SBP) in comparison to the group (SB) and the group (S). The applied statistical tests showed that the biomaterial factor, time, and interaction between them gave rise to results with statistical significance. SHED submitted to bovine biomaterial were more viable, proliferative and with lower toxicity when associated with PRF. PRF seemed to activate the metabolism of stem cells in culture, indicating that such an association can bring an effective benefit in clinical outcome.

Use of platelet- and leukocyte-rich fibrin (l-prf) in bisphosphonate-related osteonecrosis of the jaw (bronj) in rats - split-mouth study

“…For this reason, in the present study, L-PRF membrane was used as a form of prevention due to its healing properties, presence of fibrin framework with autologous cells, such as leukocytes, macrophages, neutrophils and platelets, and growth factors. (HEYMOVSKI et al, 2021;TAYSI et al, 2018). There are few reports using L-PRF for BRONJ treatment, but some studies report satisfactory results (BARBOSA et al, 2020;CANAN-DURÁN et al, 2017;PARK et al, 2017).…”

“…Studies on the use of platelet-and leukocyte-rich fibrin membranes (L-PRF) have been increasing, due to their composition, which adds growth factors (epidermal, transforming β, vascular endothelial, pro-coagulant, cytokines and antimicrobials) to the surgical site (HEYMOVSKI et al, 2021;STROPARO et al, 2021). On a daily basis, it is commonly used for maxillary sinus augmentation, bone grafting combined with biomaterials, treatment of oroantral communication and orofacial harmonization.…”

“…Given that the L-PRF membrane accelerate bone and soft tissue healing (HEYMOVSKI et al, 2021), we hypothesized that the L-PRF could aid in bone healing after tooth extractions, preventing BRONJ. Thus, the aim of this research was to clinically evaluate the effect of L-PRF membranes in preventing BRONJ after tooth extractions in rats.…”