Basolateral anion transport mechanisms underlying fluid secretion by mouse, rat and guinea‐pig pancreatic ducts

Fernández-Salazar, M. Paz; Pascua, Patricia; Calvo, José F.; López, María Rodríguez; Case, R. M.; Steward, Martin C.; Román, José Ignacio San

doi:10.1113/jphysiol.2004.061762

Cited by 52 publications

(61 citation statements)

References 38 publications

(58 reference statements)

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“…Remarkable differences in the expression of NKCC1 in the glandular epithelia of various species and organs can be observed. NKCC1 can be found in the basolateral membranes of rat and mouse pancreatic ducts, but not in the pancreatic and salivary gland duct cells of pig and guinea pig (17,40,41). Our present work evidenced the expression of NKCC1 in the basolateral membranes of both acinar and duct cells from rabbit LG by immunofluorescence with stronger staining observed in acinar cells.…”

Section: Discussionsupporting

confidence: 55%

“…In a recently published paper by our laboratory, video-microscopic technique was adapted and was used for the first time in lacrimal duct research to investigate fluid secretion of isolated LG duct segments (13). This technique was originally developed by Fernandez-Salazar et al for the measurement of fluid secretion of pancreatic ducts (14).…”

Section: Role Of Lg Ducts In Lacrimal Secretionmentioning

confidence: 99%

“…Videomicroscopic technique was used for measurement of ductal fluid secretion (13,14). Fluid secretion into closed intraluminal space of cultured lacrimal gland interlobular ducts was analyzed using the swelling method.…”

Section: Measurement Of Fluid Secretion Of Lg Ductsmentioning

confidence: 99%

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Role of transmembrane chloride transporters in the fluid secretion of lacrimal gland duct cells

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Section: Discussionsupporting

confidence: 55%

Section: Role Of Lg Ducts In Lacrimal Secretionmentioning

confidence: 99%

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Role of transmembrane chloride transporters in the fluid secretion of lacrimal gland duct cells

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“…CFTR from eutherian mammals also has a relatively low P HCO3 (Gray et al, 1993;Gray et al, 1990;Linsdell et al, 1997;O'Reilly et al, 2000;Poulsen et al, 1994). However, in the pancreatic duct cells it has been suggested that HCO 3 -secretion occurs via CFTR because there is no mechanism on the basolateral membrane to accumulate Cl -, whereas there is a mechanism for HCO 3 -accumulation (Fernández-Salazar et al, 2004). As a result, following stimulation of CFTR, the intracellular Cl -activity ([Cl -]i) rapidly falls and approaches its equilibrium value, thus there is no driving force for Cl -exit across the apical membrane via CFTR (Ishiguro et al, 2002a).…”

Section: Discussionmentioning

confidence: 99%

The distribution and expression of CFTR restricts electrogenic anion secretion to the ileum of the brushtail possum, Trichosurus vulpecula

Gill

Bartolo

Demmers

et al. 2011

Journal of Experimental Biology

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SUMMARYIn eutherian mammals, fluid secretion is essential for intestinal function. This is driven by electrogenic Cl -secretion, which involves a NaK2Cl cotransporter (NKCC1) in the enterocyte basolateral membrane and the cystic fibrosis transmembrane conductance regulator (CFTR) in the apical membrane. However, in the possum ileum, NKCC1 expression is low and secretagogues stimulate electrogenic HCO 3 -secretion driven by a basolateral NaHCO 3 cotransporter (pNBCe1). Here we investigated whether electrogenic anion secretion occurs in possum duodenum and jejunum and determined the role of CFTR in possum intestinal anion secretion. Prostaglandin E 2 (PGE 2 ) and forskolin stimulated a large increase in ileal short-circuit current (I sc ), consistent with electrogenic HCO 3 -secretion, but had little effect on the duodenal and jejunal I sc . Furthermore, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and N-(2-naphthalenyl)-[(3,5-dibromo-2,4-dihydroxyphenyl)methylene]glycine hydrazide (GlyH101) inhibited cloned possum CFTR in cultured cells and the PGE 2 -stimulated ileal I sc , implicating CFTR in ileal HCO 3 -secretion. Consistent with this, CFTR is expressed in the apical membrane of ileal crypt and lower villous cells, which also express pNBCe1 in the basolateral membrane. In contrast, duodenal and jejunal CFTR expression is low relative to the ileum. Jejunal pNBCe1 expression is also low, whereas duodenal and ileal pNBCe1 expression are comparable. All regions have low NKCC1 expression. These results indicate that cAMP-dependent electrogenic Cl -secretion does not occur in the possum small intestine because of the absence of CFTR and NKCC1. Furthermore, CFTR functions as the apical anion conductance associated with HCO 3 -secretion and its distribution limits electrogenic HCO 3 -secretion to the ileum.

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“…Latter studies isolated ducts by microdissection from pancreas of rats, guinea pigs, and mice partially digested with collagenase. Secretin has been shown to stimulate bicarbonate fluid in the isolated duct fragments by microperfusion and by letting the ends of the ducts seal in culture after which the volume increase can be monitored by video microscopy and the ion content sampled by micropuncture (3,34,46). Guinea pig ducts have been especially useful because they secrete a high concentration of bicarbonate similar to humans while rats and mice secrete lower amounts (100).…”

Section: In Vitro Studies Of Secretin Action On Pancreatic Ductsmentioning

confidence: 99%

Secretin

The Pancreapedia: Exocrine Pancreas Knowledge Base

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Secretin is one of the "classical" gastrointestinal hormones and in fact was the first peptide hormone identified. The mature hormone is a 27 amino acid peptide produced and secreted by a specific type of enteroendocrine cell, the S cell which is of the open type with its apical surface exposed to luminal content. Secretin is packaged into granules and released across the basolateral membrane into the blood in response to acid in the duodenum when the pH falls below 4.5; it is also released in response to fatty acids but without a response to glucose or amino acids. Basal and stimulated concentrations are low normally in the 1-10 pM range. The principal action of secretin is to stimulate bicarbonate secretion by pancreatic and biliary ducts and duodenal Brunner's glands into the lumen such that acid in the duodenum will be neutralized. Secretin also acts to potentiate CCK action on acinar cells, to inhibit gastric acid secretion and to inhibit gastric emptying. Secretin also has actions in the brain. At the cellular level the action of secretin is primarily mediated by cAMP which is increased in response to activation of secretin receptors.

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Basolateral anion transport mechanisms underlying fluid secretion by mouse, rat and guinea‐pig pancreatic ducts

Cited by 52 publications

References 38 publications

Role of transmembrane chloride transporters in the fluid secretion of lacrimal gland duct cells

Role of transmembrane chloride transporters in the fluid secretion of lacrimal gland duct cells

The distribution and expression of CFTR restricts electrogenic anion secretion to the ileum of the brushtail possum, Trichosurus vulpecula

Secretin

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