Basonuclines et protéines DISCO

Hervé, Françoise; Vanhoutteghem, Amandine; Djian, Philippe

doi:10.1051/medsci/2012281017

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…In particular, we have to recourse to the continuous wave ESR in a magnetic material using a coil oriented along the polarizing field direction. This method is known as LOngitudinal Detection scheme (LOD), and during the last decades it was investigated systematically, both theoretically and experimentally [14]. A magnetized spherical sample of volume V s and magnetization M 0 is placed in the bore of a solenoid, which generates a static magnetic field B 0 (polarizing field).…”

Section: Experimental Schemementioning

confidence: 99%

The QUAX proposal: a search of galactic axion with magnetic materials

Ruoso

Lombardi

Ortolan

et al. 2016

J. Phys.: Conf. Ser.

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Aim of the QUAX (QUaerere AXion) proposal is to exploit the interaction of cosmological axions with the spin of electrons in a magnetized sample. Their effect is equivalent to the application of an oscillating rf field with frequency and amplitude which are fixed by axion mass and coupling constant, respectively. The rf receiver module of the QUAX detector consists of magnetized samples with the Larmor resonance frequency tuned to the axion mass by a polarizing static magnetic field. The interaction of electrons with the axion-equivalent rf field produces oscillations in the total magnetization of the samples. To amplify such a tiny field, a pump field at the same frequency is applied in a direction orthogonal to the polarizing field. The induced oscillatory magnetization along the polarizing field is measured by a SQUID amplifier operated at its quantum noise level.

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Section: Experimental Schemementioning

confidence: 99%

The QUAX proposal: a search of galactic axion with magnetic materials

Ruoso

Lombardi

Ortolan

et al. 2016

J. Phys.: Conf. Ser.

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The importance of basonuclin 2 in adult mice and its relation to basonuclin 1

Vanhoutteghem

Delhomme

Hervé

et al. 2016

Mechanisms of Development

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BNC2 is an extremely conserved zinc finger protein with important functions in the development of craniofacial bones and male germ cells. Because disruption of the Bnc2 gene in mice causes neonatal lethality, the function of the protein in adult animals has not been studied. Until now BNC2 was considered to have a wider tissue distribution than its paralog, BNC1, but the precise cell types expressing Bnc2 are largely unknown. We identify here the cell types containing BNC2 in the mouse and we show the unexpected presence of BNC1 in many BNC2-containing cells. BNC1 and BNC2 are colocalized in male and female germ cells, ovarian epithelial cells, sensory neurons, hair follicle keratinocytes and connective cells of organ capsules. In many cell lineages, the two basonuclins appear and disappear synchronously. Within the male germ cell lineage, BNC1 and BNC2 are found in prospermatogonia and undifferentiated spermatogonia, and disappear abruptly from differentiating spermatogonia. During oogenesis, the two basonuclins accumulate specifically in maturing oocytes. During the development of hair follicles, BNC1 and BNC2 concentrate in the primary hair germs. As follicle morphogenesis proceeds, cells possessing BNC1 and BNC2 invade the dermis and surround the papilla. During anagen, BNC1 and BNC2 are largely restricted to the basal layer of the outer root sheath and the matrix. During catagen, the compartment of cells possessing BNC1 and BNC2 regresses, and in telogen, the two basonuclins are confined to the secondary hair germ. During the next anagen, the BNC1/BNC2-containing cell population regenerates the hair follicle. By examining Bnc2(-/-) mice that have escaped the neonatal lethality usually associated with lack of BNC2, we demonstrate that BNC2 possesses important functions in many of the cell types where it resides. Hair follicles of postnatal Bnc2(-/-) mice do not fully develop during the first cycle and thereafter remain blocked in telogen. It is concluded that the presence of BNC2 in the secondary hair germ is required to regenerate the transient segment of the follicle. Postnatal Bnc2(-/-) mice also show severe dwarfism, defects in oogenesis and alterations of palatal rugae. Although the two basonuclins possess very similar zinc fingers and are largely coexpressed, BNC1 cannot substitute for BNC2. This is shown incontrovertibly in knockin mice expressing Bnc1 instead of Bnc2 as these mice invariably die at birth with craniofacial abnormalities undistinguishable from those of Bnc2(-/-) mice. The function of the basonuclins in the secondary hair germ is of particular interest.

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The zinc-finger protein basonuclin 2 is required for proper mitotic arrest, prevention of premature meiotic initiation and meiotic progression in mouse male germ cells

et al. 2014

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Absence of mitosis and meiosis are distinguishing properties of male germ cells during late fetal and early neonatal periods. Repressors of male germ cell meiosis have been identified, but mitotic repressors are largely unknown, and no protein repressing both meiosis and mitosis is known. We demonstrate here that the zinc-finger protein BNC2 is present in male but not in female germ cells. In testis, BNC2 exists as several spliced isoforms and presumably binds to DNA. Within the male germ cell lineage, BNC2 is restricted to prospermatogonia and undifferentiated spermatogonia. Fetal prospermatogonia that lack BNC2 multiply excessively on embryonic day (E)14.5 and reenter the cell cycle prematurely. Mutant prospermatogonia also engage in abnormal meiosis; on E17.5, Bnc2−/− prospermatogonia start synthesizing the synaptonemal protein SYCP3, and by the time of birth, many Bnc2 −/− prospermatogonia have accumulated large amounts of nonfilamentous SYCP3, thus appearing to be blocked at leptonema. Bnc2 −/− prospermatogonia do not undergo proper male differentiation, as they lack almost all the mRNA for the malespecific methylation protein DNMT3L and have increased levels of mRNAs that encode meiotic proteins, including STRA8. Bnc2 −/− prospermatogonia can produce spermatogonia, but these enter meiosis prematurely and undergo massive apoptotic death during meiotic prophase. This study identifies BNC2 as a major regulator of male germ stem cells, which is required for repression of meiosis and mitosis in prospermatogonia, and for meiosis progression during spermatogenesis. In view of the extreme evolutionary conservation of BNC2, the findings described here are likely to apply to many species.

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Basonuclines et protéines DISCO

Cited by 4 publications

References 31 publications

The QUAX proposal: a search of galactic axion with magnetic materials

The QUAX proposal: a search of galactic axion with magnetic materials

The importance of basonuclin 2 in adult mice and its relation to basonuclin 1

The zinc-finger protein basonuclin 2 is required for proper mitotic arrest, prevention of premature meiotic initiation and meiotic progression in mouse male germ cells

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