Basophil Activation Test: Old and New Applications in Allergy

Hemmings, Oliver; Kwok, Matthew; McKendry, Richard; Santos, Alexandra

doi:10.1007/s11882-018-0831-5

Cited by 146 publications

(149 citation statements)

References 125 publications

(137 reference statements)

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“…The basophil activation test has been reported to add significant diagnostic value to IgE-based test methods and clinical histories. [73][74][75] In a small number of patients (11 peanut-allergic and 14 nonallergic),…”

Section: Pbmcs Of Milk-allergic Individuals Show a Significant Downrementioning

confidence: 99%

Recent developments and highlights in food allergy

Eiwegger

Hung

Diego

et al. 2019

Allergy

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The achievement of long-lasting, safe treatments for food allergy is dependent on the understanding of the immunological basis of food allergy. Accurate diagnosis is essential for management. In recent years, data from oral food challenges have revealed that routine allergy testing is poor at predicting clinical allergy for tree nuts, almonds in particular. More advanced antigen-based tests including component-resolved diagnostics and epitope reactivity may lead to more accurate diagnosis and selection of therapeutic intervention. Additional diagnostic accuracy may come from cellular tests such as the basophil activation test or mast cell approaches. In the context of clinical trials, cellular tests have revealed specific T-cell and B-cell populations that are more abundant in food-allergic individuals with distinct mechanistic features.Awareness of clinical markers, such as the ability to eat baked forms of milk and egg, continues to inform the understanding of natural tolerance development.Mouse models have allowed for investigation into multiple mechanisms of food allergy including modification of epithelial metabolism, and the induction of regulatory cell subsets and the microbiome. Increasing numbers of children who underwent food immunotherapy enlarged the body of evidence on mechanisms and predictors of treatment success. Experimental immunological markers in conjunction with clinical determinants such as lower age and lower initial specific IgE appear to be of benefit.More research on the optimal dose, preparation, and route of application integrating a high-level safety and efficacy is demanded. Alternatively, biologics blocking TSLP, IL-33, IL-4 and IL-13, or IgE may help to achieve that. K E Y W O R D Sallergy treatment, biologics, food allergy, immune tolerance, immunotherapy | 2357 EIWEGGER Et al.

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Section: Pbmcs Of Milk-allergic Individuals Show a Significant Downrementioning

confidence: 99%

Recent developments and highlights in food allergy

Eiwegger

Hung

Diego

et al. 2019

Allergy

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“…Fungal species demonstrated as triggers of allergic pulmonary diseases have been reviewed previously (17,18), while cellular assays for ABPM diagnosis were reported as early as 1977, as described in Table 2 (19)(20)(21)(22)25). BAT can demonstrate an immediate-hypersensitivity mechanism (8,9). Only three studies have evaluated the utility of BAT for ABPA diagnosis so far.…”

Section: Discussion and Reviewmentioning

confidence: 99%

Evaluation of Cellular Responses for the Diagnosis of Allergic Bronchopulmonary Mycosis: A Preliminary Study in Cystic Fibrosis Patients

Michel

Gomez²,

Sérémé

et al. 2020

Front. Immunol.

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Results: BAT responses of ABPA patients were higher than those of sensitized or control CF patients. The highest LST result was for a woman who developed ABPA 3 months after the tests, despite the absence of specific IgG and IgE to Af at the time of the initial investigation. Michel et al. Cellular Responses of ABPM Conclusion: We conclude that basophil and lymphocyte activation tests could enhance the diagnosis of allergic mycosis, compared to usual humoral markers. Further studies with larger cohorts and addressing both mold extracts and mold relevant molecules are needed in order to confirm and extend the application of this personalized medicine approach.

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“…Activated basophils are important biomarkers of allergic responses, reflecting specific IgE functional activity. In vitro basophil activation tests (BAT) may be useful in monitoring patients on AIT, by ex vivo measurements of CD63 or CD203c expression, basophil histamine release (BHR) or diamine oxidase (DAO), avoiding the risk of severe allergic reactions with in vivo allergen challenge . However, advances in practical aspects of the test are required for standardization and adaptation for use in routine laboratories.…”

Section: Candidate Biomarkers For Immune Monitoring In Allergen Immunmentioning

confidence: 99%

“…Information that can be obtained from sequence analysis is highlighted vivo allergen challenge. [94][95][96][97] However, advances in practical aspects of the test are required for standardization and adaptation for use in routine laboratories. Due to their sequestration into tissues, mast cells remain difficult to investigate in the context of AIT and immune monitoring.…”

Section: Immunoglobulin Transcriptsmentioning

confidence: 99%

Recent developments and highlights in immune monitoring of allergen immunotherapy

Zelm

McKenzie

Varese

et al. 2019

Allergy

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Allergic diseases are the most common chronic immune-mediated disorders and can manifest with an enormous diversity in clinical severity and symptoms. Underlying mechanisms for the adverse immune response to allergens and its downregulation by treatment are still being revealed. As a result, there have been, and still are, major challenges in diagnosis, prediction of disease progression/evolution and treatment.Currently, the only corrective treatment available is allergen immunotherapy (AIT).AIT modifies the immune response through long-term repeated exposure to defined doses of allergen. However, as the treatment usually needs to be continued for several years to be effective, and can be accompanied by adverse reactions, many patients face difficulties completing their schedule. Long-term therapy also potentially incurs high costs. Therefore, there is a great need for objective markers to predict or to monitor individual patient's beneficial changes in immune response during therapy so that efficacy can be identified as early as possible. In this review, we specifically address recent technical developments that have generated new insights into allergic disease pathogenesis, and how these could potentially be translated into routine laboratory assays for disease monitoring during AIT that are relatively inexpensive, robust and scalable. K E Y W O R D Sallergen immunotherapy, allergy, biomarker, immune monitoring | 2343 ZELM Et aL.

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Basophil Activation Test: Old and New Applications in Allergy

Cited by 146 publications

References 125 publications

Recent developments and highlights in food allergy

Recent developments and highlights in food allergy

Evaluation of Cellular Responses for the Diagnosis of Allergic Bronchopulmonary Mycosis: A Preliminary Study in Cystic Fibrosis Patients

Recent developments and highlights in immune monitoring of allergen immunotherapy

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