Basophil histamine release tests in the diagnosis of allergy and asthma

Crockard, A.D.; Ennis, Madeleine

doi:10.1046/j.1365-2222.2001.01043.x

Cited by 40 publications

(38 citation statements)

References 22 publications

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“…Histamine release can be a criterion for the diagnosis of food allergy (Crockard & Ennis 2001) as patients manifest increased the release of histamine from the gastrointestinal mucosa after being sensitised by food allergen (He et al 2004). Intracellular histamine levels can be used as an indicator of the sensitivity to histamine release, which means that lower are intracellular histamine levels, more histamine is released by proliferative mast cells, and more histamine participates in the metabolism of the small intestine (Sun et al 2008b).…”

Section: Discussionmentioning

confidence: 99%

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Cao

Meng

et al. 2016

Italian Journal of Animal Science

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Glycinin, an antigenic glycoprotein found in soybeans, is the major cause of allergic reactions in young animals. The objective of this study was to evaluate the effects of a prior immunisation of sucking piglets with glycinin on their post-weaning growth performance, serum immunoglobulin contents, small intestinal histamine release and mucosal histology. Forty piglets (7 d of age) were randomly divided into four groups of 10 piglets each. Piglets of Group C (Control) received a physiological saline solution, Groups Im (Immunised) and Im þ S (Sensitised) were immunised twice with 500 lg/kg of glycinin at 7 and 21-d-old. All piglets were weaned at 23 d; Groups Im þ S and S were sensitised with 2500 lg/kg of glycinin at weaning. Compared with Group C, in Group S the average daily gain (ADG), average daily feed intake and gain:feed ratio were decreased, and serum levels of IgG and IgE were increased. Furthermore, in this group, the histamine levels in the duodenum and ileum were significantly decreased, and the structure of duodenal and ileal mucosa was severely damaged. On the contrary, in Groups Im and Im þ S the ADG was increased, serum IgE levels were decreased, intestinal histamine levels were increased and the intestinal mucosa was not damaged. These findings suggest that prior immunisation with glycinin can protect the structural integrity of the intestinal mucosal epithelia and alleviate allergic reactions in piglets.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Cao

Meng

et al. 2016

Italian Journal of Animal Science

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“…Furthermore, skin prick testing does not always correlate with immunochemical analyses [13, 14] and may also be false positive or negative. Mediator release can also be measured by the basophil assay [15, 16]. However, this test depends on fresh blood samples because of the absence of suitable long-term human basophil cultures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an in vitro Mast Cell Degranulation Test in the Context of Food Allergy to Wheat

Bodinier

Brossard

Triballeau

et al. 2008

Int Arch Allergy Immunol

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Background: Antigenic profiles obtained by ELISA with IgE from patients with wheat food allergy (WFA) established that major allergens are albumins/globulins (AG) for children suffering from atopic eczema/dermatitis syndrome (AEDS), ω5-gliadins for adults suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA), anaphylaxis or urticaria and low-molecular-weight (LMW) glutenin subunits for patients with anaphylaxis. We aimed to characterize a new mast cell transfectant for its ability to degranulate with wheat proteins and patient sera and compare these results to those obtained by ELISA. Methods: Thirty sera from patients with WFA were tested: 14 with AEDS (group 1) and 16 with WDEIA, anaphylaxis or urticaria (group 2). An IgE Fc receptor (FcΕRI) humanized rat RBL-2H3 line was established by transfection with cDNAs encoding α-, β- and γ-subunits for the human IgE receptor. Results: A humanized RBL clone was selected for its capacity to express mRNA α-, β- and γ-subunits of FcΕRI, to bind allergen-specific human IgE and to degranulate. In group 1, sera induced enhanced degranulation with AG extract, but rarely reacted with gliadins and glutenins. In group 2, half of the sera showed degranulation with LMW glutenins whereas the AG fraction and lipid transfer proteins were rarely positive. ω5-Gliadins did not appear as a major allergen in degranulation assays, although functional allergen-specific IgE was measurable in appreciable amounts. Conclusion: Our data demonstrate that in wheat food allergen evaluation, correlation exists between mast cell degranulation and IgE measurements, depending on the type of allergen. Therefore, the biological activity of some allergen types may also be affected by other parameters.

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“…Consequently, both skin tests (SPT, SAFT) and the determination of asIgE levels in blood serum may play a rather insignificant role in the diagnosis of eHF. So far positive and negative predictive values (PPV and NPV) find application only in the diagnostics of CMPA [2,61], as is the case with cellular activation tests, the histamine release microfiber method (HR-MM), the cellular antigen stimulator test (CAST-ELISA) or the basophil activation test (BAT) with CD63 and CD203c molecules -all used in the diagnosis of the non-IgE-mediated immune mechanism [62][63][64]. When suspecting allergy to eHF a challenge test should therefore be carried out.…”

Section: Discussionmentioning

confidence: 99%

Patient characteristics of children with cow’s milk allergy presenting with an allergy to extensively hydrolyzed whey and/or casein formulae used as part of an elimination diet

Nowacki¹

2012

pdia

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A Ad dd dr re es ss s f fo or r c co or rr re es sp po on nd de en nc ce e: : Zygmunt Nowacki MD, PhD, Allergological-Pediatric Practice, Original paper A b s t r a c t I In nt tr ro od du uc ct ti io on n: : In the recent years the number of children with cow's milk protein allergy (CMPA) presenting with allergies to extensively hydrolyzed whey (eHF-W) and casein formulae (eHF-C) respectively has increased. A Ai im m: : To present the patient characteristics of children with CMPA who presented with allergic reactions to eHF-W and/or eHF-C used as part of an elimination diet. M Ma at te er ri ia al l a an nd d m me et th ho od ds s: : A retrospective study was carried out for a group of 65 selected from of 359 children (3 to 12 months) affected with CMPA and allergy to eHF. R Re es su ul lt ts s: : 18.1% (65/359) and 7.8% (28/359) from the group presented with allergic reactions to eHF-W and eHF-C, respectively. An allergic reaction to eHF-C occurred in 43% of the patients from the group (28/65). IgE-mediated CMPA presented with one of the following clinical manifestations: atopic dermatitis (AD) 94%, gastrointestinal symptoms (GI) 47%, respiratory symptoms (RS) 44%, anaphylactic reactions (AR) 26%. Non-IgE-mediated CMPA, on the other hand, manifested with: AD 97%, GI 22%, RS 10% .The observations showed that all 31 from the non-IgE mediated CMPA group with GI and AD manifestations presented with symptoms of eHF-C allergy (p = 0.001). In the IgE-mediated CMPA (n = 34) the following factors were statistically significant with relation to eHF-C allergy: CMPA manifesting with GI (p = 0.014) and AD. The occurrence of AR in response to cow's milk protein (CMP) decreased the probability of an eHF-C allergy (p = 0.028) in a statistically significant way. C Co on nc cl lu us si io on ns s: : Children with CMPA who presented with allergies to eHF-W and eHF-C had a higher frequency of clinical manifestation with GI and AD. K Ke ey y w wo or rd ds s: : cow's milk allergy, elimination diet.

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Basophil histamine release tests in the diagnosis of allergy and asthma

Cited by 40 publications

References 22 publications

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Evaluation of an in vitro Mast Cell Degranulation Test in the Context of Food Allergy to Wheat

Patient characteristics of children with cow’s milk allergy presenting with an allergy to extensively hydrolyzed whey and/or casein formulae used as part of an elimination diet

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