Basophils and IgE contribute to mixed connective tissue disease development

Lamri, Yasmine; Vibhushan, Shamila; Pacreau, Emeline; Boedec, Erwan; Saidoune, Fanny; Mailleux, Arnaud; Crestani, Bruno; Blank, Ulrich; Benhamou, Marc; Papo, Thomas; Daugas, Éric; Sacré, Karim; Charles, Nicolas

doi:10.1016/j.jaci.2020.12.622

Cited by 18 publications

(28 citation statements)

References 35 publications

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“…The reduction of circulating basophils may be an intrinsic characteristic of the pSLE phenotype, even though some authors linked this aspect to the disease activity, which would promote their extravasation in the inflamed peripheral tissues [ 18 , 19 , 21 , 22 , 23 ]. Notably, our data showed a mild, but significant, difference between pSLE children with low–moderate and high–very high disease activity, according to SLEDAI, even though we could not demonstrate a similar correlation with the alteration of the inflammatory parameters separately.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Peripheral Blood Basophils in Pediatric Systemic Lupus Erythematosus

et al. 2022

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Basophils are the least abundant circulating leukocytes, and their immunological role has not yet been completely elucidated. There is evidence supporting their immunomodulatory role in several pathological settings; recently, studies in both experimental models and humans suggested that basophil homeostasis may be altered in systemic lupus erythematosus (SLE). Here, we first assessed circulating basophils in children affected with pediatric SLE (pSLE). In this cross-sectional study, circulating basophils were enumerated by fluorescence-based flow cytometry analysis in children affected with pSLE, in addition to children suffering from juvenile idiopathic arthritis (JIA) or non-inflammatory/non-rheumatic conditions. This study included 52 pediatric patients distributed in these three groups. We observed a statistically significant reduction of peripherally circulating basophils in children with pSLE compared to the other two groups of patients. This preliminary study is consistent with the available studies in adult patients with SLE showing a reduced number of circulating basophils. However, further research is needed to draw final conclusions on basophils’ homeostasis in pSLE, in addition to their correlation with the disease activity and concomitant therapies.

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Section: Discussionmentioning

confidence: 99%

Analysis of Peripheral Blood Basophils in Pediatric Systemic Lupus Erythematosus

et al. 2022

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“…Si les atteintes tissulaires des MAPA ont été clairement associées aux sous-groupes de lymphocytes T auxiliaires (T helper [Th] cells) Th1 et Th17, la composante Th2 de ces maladies, qui contribue à la production des Ac, et donc des AAc, a longtemps été ignorée. Nous résumerons dans cette revue les travaux qui ont permis de révéler l'importance des granulocytes basophiles et des immunoglobulines de type E (IgE) dans l'amplification et l'instauration de conditions autoimmunes, notamment dans deux MAPA, le lupus érythémateux disséminé (LED) et la connectivite mixte (mixed connective tissue disease ou MCTD) [9][10][11].…”

Section: Mécanismes Et Cibles Thérapeutiquesunclassified

“…Basophiles celle du LED, est associée à l'accumulation de lymphocytes T CD4 + et de lymphocytes B autoréactifs, à la pathogénicité des AAc anti-U1-snRNP, et aux lésions vasculaires qui leur sont associées [10,31,32]. Comme chez les patients lupiques, les patients atteints de MCTD présentent une basopénie périphérique.…”

Section: Ptgdr Cxcr4unclassified

“…Leurs granulocytes basophiles ont un phénotype activé qui diffère cependant du phénotype retrouvé pour les granulocytes basophiles des patients lupiques. Une différence majeure consiste en la surexpression à leur surface de CCR3 (C-C chemokine receptor type 3), le récepteur des chimiokines CCL (C-C motif chemokine ligand)11 et 5, entre autres [10]. En plus des IgG anti-U1-snRNP, présentes chez tous les patients, des IgE autoréactives anti-U1-snRNP ont été également retrouvées chez plus de 80 % des patients atteints de MCTD, suggérant que, comme dans le LED, les granulocytes basophiles et les IgE autoréactives pourraient être impliqués dans la physiopathologie de la maladie [10].…”

Section: Ptgdr Cxcr4unclassified

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Les granulocytes basophiles et les IgE dans l’autoimmunité

Tchen

Charles

2022

Med Sci (Paris)

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Comprendre les mécanismes physiopathologiques des maladies autoimmunes présentant des auto-anticorps (MAPA) représente un enjeu majeur pour le développement d’approches thérapeutiques innovantes. Sans en connaître précisément les origines, chez certains individus, la perte de tolérance à des antigènes du soi conduit à l’instauration d’une réaction en chaîne autoimmune. Des autoanticorps sont cependant présents dans une large proportion de la population générale sans être associés à une maladie. L’amplification de la production de ces autoanticorps, la formation de complexes immuns circulants et l’activation du système immunitaire inné menant à cette amplification sont des processus centraux dans la pathogénie des maladies auto-immunes. Dans cette revue, nous présentons la contribution de l’immunité de type 2, des granulocytes basophiles et des IgE, dans l’instauration de boucles d’amplification pathogéniques dans les MAPA, en particulier dans le lupus érythémateux disséminé et la connectivite mixte.

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“…Mast cells and basophils are important cells of the immune system ( 1 – 3 ) and play critical roles in several allergic ( 4 – 9 ) and autoimmune disorders ( 10 – 12 ), infections ( 13 , 14 ), cardiovascular diseases ( 15 – 17 ), immunodeficiencies ( 18 ), and cancer ( 19 – 22 ). The secretion of preformed mediators (e.g.…”

Section: Introductionmentioning

confidence: 99%

IgG Autoantibodies Against IgE from Atopic Dermatitis Can Induce the Release of Cytokines and Proinflammatory Mediators from Basophils and Mast Cells

et al. 2022

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IgE-mediated release of proinflammatory mediators and cytokines from basophils and mast cells is a central event in allergic disorders. Several groups of investigators have demonstrated the presence of autoantibodies against IgE and/or FcεRI in patients with chronic spontaneous urticaria. By contrast, the prevalence and functional activity of anti-IgE autoantibodies in atopic dermatitis (AD) are largely unknown. We evaluated the ability of IgG anti-IgE from patients with AD to induce the in vitro IgE-dependent activation of human basophils and skin and lung mast cells. Different preparations of IgG anti-IgE purified from patients with AD and rabbit IgG anti-IgE were compared for their triggering effects on the in vitro release of histamine and type 2 cytokines (IL-4, IL-13) from basophils and of histamine and lipid mediators (prostaglandin D2 and cysteinyl leukotriene C4) from human skin and lung mast cells. One preparation of human IgG anti-IgE out of six patients with AD induced histamine release from basophils, skin and lung mast cells. This preparation of human IgG anti-IgE induced the secretion of cytokines and eicosanoids from basophils and mast cells, respectively. Human monoclonal IgE was a competitive antagonist of both human and rabbit IgG anti-IgE. Human anti-IgE was more potent than rabbit anti-IgE for IL-4 and IL-13 production by basophils and histamine, prostaglandin D2 and leukotriene C4 release from mast cells. Functional anti-IgE autoantibodies rarely occur in patients with AD. When present, they induce the release of proinflammatory mediators and cytokines from basophils and mast cells, thereby possibly contributing to sustained IgE-dependent inflammation in at least a subset of patients with this disorder.

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Basophils and IgE contribute to mixed connective tissue disease development

Cited by 18 publications

References 35 publications

Analysis of Peripheral Blood Basophils in Pediatric Systemic Lupus Erythematosus

Analysis of Peripheral Blood Basophils in Pediatric Systemic Lupus Erythematosus

Les granulocytes basophiles et les IgE dans l’autoimmunité

IgG Autoantibodies Against IgE from Atopic Dermatitis Can Induce the Release of Cytokines and Proinflammatory Mediators from Basophils and Mast Cells

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